##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727363.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2751646
Sequences flagged as poor quality	0
Sequence length	43
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.11436718240646	33.0	31.0	34.0	30.0	34.0
2	32.256939301058345	33.0	31.0	34.0	30.0	34.0
3	32.332755012817785	34.0	31.0	34.0	30.0	34.0
4	35.910600418803874	37.0	35.0	37.0	35.0	37.0
5	35.79132272101862	37.0	35.0	37.0	35.0	37.0
6	35.81394808779908	37.0	35.0	37.0	35.0	37.0
7	35.75842350360475	37.0	35.0	37.0	35.0	37.0
8	35.7334787977814	37.0	35.0	37.0	33.0	37.0
9	37.45960454215404	39.0	37.0	39.0	35.0	39.0
10	37.30225545001065	39.0	37.0	39.0	34.0	39.0
11	37.4099073790742	39.0	37.0	39.0	35.0	39.0
12	37.32079344508705	39.0	37.0	39.0	34.0	39.0
13	37.382830131492206	39.0	37.0	39.0	34.0	39.0
14	38.52919089156091	40.0	38.0	41.0	34.0	41.0
15	38.499738338434526	40.0	38.0	41.0	34.0	41.0
16	38.42994229635644	40.0	38.0	41.0	34.0	41.0
17	38.40085025472027	40.0	38.0	41.0	34.0	41.0
18	38.36663582452103	40.0	38.0	41.0	34.0	41.0
19	38.3806561599857	40.0	38.0	41.0	34.0	41.0
20	38.27134158972484	40.0	38.0	41.0	34.0	41.0
21	38.197783799224176	40.0	38.0	41.0	34.0	41.0
22	38.12823633563329	40.0	38.0	41.0	33.0	41.0
23	38.01343850190032	40.0	37.0	41.0	33.0	41.0
24	37.91638931752122	40.0	37.0	41.0	33.0	41.0
25	37.83617878171829	40.0	37.0	41.0	33.0	41.0
26	37.48258678623631	39.0	37.0	41.0	32.0	41.0
27	37.26902116042543	39.0	37.0	41.0	31.0	41.0
28	37.12486744297777	39.0	36.0	40.0	31.0	41.0
29	37.06674368723302	39.0	36.0	40.0	31.0	41.0
30	36.989759220481126	39.0	36.0	40.0	31.0	41.0
31	36.77764872370937	39.0	36.0	40.0	30.0	41.0
32	36.672006500836225	39.0	36.0	40.0	30.0	41.0
33	36.486698870421556	39.0	35.0	40.0	30.0	41.0
34	36.35384130080686	39.0	35.0	40.0	30.0	41.0
35	36.199512219231686	39.0	35.0	40.0	29.0	41.0
36	36.05041055426461	38.0	35.0	40.0	29.0	41.0
37	35.810494518553625	38.0	35.0	40.0	27.0	41.0
38	35.704757806781835	38.0	35.0	40.0	27.0	41.0
39	35.545195857315946	38.0	35.0	40.0	26.0	41.0
40	35.39519291362333	38.0	34.0	40.0	26.0	41.0
41	35.24874784038354	38.0	34.0	40.0	25.0	41.0
42	35.031273281519496	38.0	34.0	40.0	24.0	41.0
43	31.714363330166744	35.0	29.0	38.0	15.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	13.0
11	15.0
12	10.0
13	8.0
14	24.0
15	23.0
16	41.0
17	93.0
18	260.0
19	478.0
20	1099.0
21	2030.0
22	3674.0
23	6077.0
24	9518.0
25	13776.0
26	19067.0
27	25998.0
28	33938.0
29	43987.0
30	55487.0
31	70217.0
32	87624.0
33	110776.0
34	142661.0
35	189368.0
36	265852.0
37	420809.0
38	735725.0
39	512996.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.198487741519074	19.02025187833028	13.340814915872173	25.440445464278472
2	18.3958983095936	21.859098154341076	34.66583274156632	25.079170794499
3	18.854714596281642	22.46964180712199	31.002970585605855	27.67267301099051
4	14.936005576298694	15.787750313812168	33.19881990633969	36.07742420354944
5	14.669982984729868	37.21045512395126	33.68543773435972	14.434124156959143
6	36.049586320333354	35.246830442578734	14.032546337719316	14.671036899368598
7	29.73583811289679	31.031644332156098	20.321364012667328	18.911153542279784
8	28.22132643515917	35.25002852837901	18.661157721596457	17.867487314865357
9	26.732108708751053	14.04977239077992	18.48021148069192	40.73790741977711
10	15.892960068264594	28.214603186601767	33.336810040245	22.55562670488864
11	36.79146954223036	21.51170608428555	20.896801405413342	20.800022968070746
12	21.42444922057561	25.644577827235043	29.91896486684697	23.012008085342373
13	32.01127615979672	19.650674541710668	23.79190491800181	24.5461443804908
14	22.292584147815525	20.528367384467334	26.16190454731459	31.01714392040255
15	25.71896966397567	28.340782208176492	21.826899245033697	24.113348882814144
16	24.602038198227532	26.20613262025711	24.254936863244765	24.936892318270594
17	24.13787965457766	26.74130320542686	25.587266676018643	23.533550463976834
18	24.22230185132826	25.681028736981425	26.08427828288959	24.012391128800726
19	25.009903163415643	25.940073686804187	26.21921569853099	22.83080745124918
20	24.658586169877957	25.660713623772825	25.85070899381679	23.829991212532427
21	24.12279777267861	26.232189751152585	25.881308860224028	23.763703615944785
22	24.8573399339886	25.668636154505343	25.277633823536895	24.196390087969164
23	24.268928488620993	25.578762675140627	25.512947523046204	24.639361313192175
24	24.262350607599963	25.64683102404888	25.6362191938934	24.454599174457762
25	24.564678741378795	25.279305550205223	25.553868484536164	24.60214722387982
26	24.111168369768496	25.610852558795717	26.088821018401347	24.189158053034436
27	24.631547808111947	25.96500421929274	25.62851471446545	23.77493325812986
28	24.379480500035253	26.024386857902503	25.564989101068957	24.031143540993284
29	24.184978736363615	26.253013650738506	25.956282167110157	23.60572544578772
30	24.315482442145537	25.97274504060479	25.754657394156077	23.957115123093594
31	24.12334290094002	25.455672713713902	25.91924978721827	24.50173459812781
32	23.759378931737587	25.82632358958965	25.82279842683252	24.591499051840245
33	24.03921143926217	25.501427145788373	25.717661356148287	24.741700058801168
34	24.267184078184474	25.356204976948344	26.176404959068133	24.200205985799045
35	23.94421375424019	25.37077807247008	26.29702367237646	24.38798450091327
36	23.821123792813463	24.956335226260936	26.632204869376363	24.59033611154923
37	23.330690066963555	24.51140153929684	27.099089054333298	25.058819339406302
38	23.19099186450583	24.477131142596104	27.530212825341632	24.801664167556435
39	22.869075455200267	24.282484011388092	28.072433736025637	24.776006797386003
40	23.034939814205753	24.246396520482648	28.39954703475665	24.319116630554948
41	22.724798175346685	24.079878007563472	28.623013280051286	24.572310537038557
42	22.077912638471663	24.50093507667774	28.65608439457692	24.765067890273677
43	22.250427562266367	23.67510210252336	28.49025637745553	25.58421395775474
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1262.0
1	1026.5
2	791.0
3	1536.0
4	2281.0
5	2281.0
6	2601.0
7	2921.0
8	3059.5
9	3198.0
10	4977.5
11	6757.0
12	6757.0
13	11769.5
14	16782.0
15	25152.5
16	33523.0
17	25595.5
18	17668.0
19	17668.0
20	19845.0
21	22022.0
22	19384.5
23	16747.0
24	20085.5
25	23424.0
26	23424.0
27	28640.0
28	33856.0
29	38260.0
30	42664.0
31	49854.0
32	57044.0
33	57044.0
34	65608.0
35	74172.0
36	85512.0
37	96852.0
38	109961.0
39	123070.0
40	123070.0
41	137315.5
42	151561.0
43	166701.0
44	181841.0
45	196151.5
46	210462.0
47	210462.0
48	225192.5
49	239923.0
50	246212.0
51	252501.0
52	252337.0
53	252173.0
54	252173.0
55	237724.5
56	223276.0
57	204056.5
58	184837.0
59	167986.0
60	151135.0
61	151135.0
62	131784.0
63	112433.0
64	94908.0
65	77383.0
66	64079.0
67	50775.0
68	50775.0
69	41542.5
70	32310.0
71	26614.0
72	20918.0
73	17155.5
74	13393.0
75	13393.0
76	11040.0
77	8687.0
78	7048.0
79	5409.0
80	4351.0
81	3293.0
82	3293.0
83	2575.0
84	1857.0
85	1482.5
86	1108.0
87	924.0
88	740.0
89	740.0
90	540.0
91	340.0
92	255.0
93	170.0
94	113.5
95	57.0
96	57.0
97	38.0
98	19.0
99	15.0
100	11.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	2751646.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	62.31068166199748
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.71236603544371	51.53863909545146
2	9.281771170111346	11.567069772806281
3	2.9985586098420947	5.605266929481374
4	1.4339986517234118	3.5741373396508442
5	0.8651397230970088	2.695372293952319
6	0.5702910676323247	2.132113510195108
7	0.3938205667279796	1.7177459575734202
8	0.2713817580678428	1.3527985868670858
9	0.22460687532657786	1.2595866756813314
>10	1.1862109957239595	12.86377648266908
>50	0.04609319324452277	1.9111536227937118
>100	0.014243757645016387	1.5599050005911532
>500	8.755358159371339E-4	0.39141561534250136
>1k	4.085833807054876E-4	0.35195399639816277
>5k	1.751071631594947E-4	0.7445334566773576
>10k+	5.836905438649823E-5	0.7345316638686882
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	20196	0.7339606911644885	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	7421	0.2696931218623326	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7332	0.2664586941779575	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	5718	0.2078028932500765	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	3.634188409410222E-5	0.0	0.0	0.0	0.0
3	3.634188409410222E-5	0.0	0.0	0.0	0.0
4	3.634188409410222E-5	0.0	0.0	0.0	0.0
5	3.634188409410222E-5	0.0	0.0	0.0	0.0
6	3.634188409410222E-5	0.0	0.0	0.0	3.634188409410222E-5
7	3.634188409410222E-5	0.0	0.0	0.0	3.634188409410222E-5
8	3.634188409410222E-5	0.0	0.0	0.0	3.634188409410222E-5
9	3.634188409410222E-5	0.0	0.0	3.634188409410222E-5	1.4536753637640887E-4
10	3.634188409410222E-5	0.0	0.0	7.268376818820444E-5	1.4536753637640887E-4
11	3.634188409410222E-5	0.0	0.0	7.268376818820444E-5	1.4536753637640887E-4
12	3.634188409410222E-5	0.0	0.0	1.4536753637640887E-4	1.4536753637640887E-4
13	3.634188409410222E-5	0.0	0.0	1.4536753637640887E-4	1.8170942047051112E-4
14	3.634188409410222E-5	0.0	0.0	1.8170942047051112E-4	2.9073507275281774E-4
15	3.634188409410222E-5	0.0	0.0	3.997607250351244E-4	2.9073507275281774E-4
16	3.634188409410222E-5	0.0	0.0	8.722052182584533E-4	3.6341884094102223E-4
17	3.634188409410222E-5	0.0	0.0	0.0011992821751053732	3.997607250351244E-4
18	3.634188409410222E-5	3.634188409410222E-5	0.0	0.0013809915955758844	3.997607250351244E-4
19	1.0902565228230667E-4	3.634188409410222E-5	0.0	0.0019261198569874177	3.997607250351244E-4
20	1.0902565228230667E-4	3.634188409410222E-5	0.0	0.002907350727528178	3.997607250351244E-4
21	1.0902565228230667E-4	3.634188409410222E-5	0.0	0.004215658554915858	4.7244449322332886E-4
22	1.0902565228230667E-4	3.634188409410222E-5	0.0	0.005851043339150458	4.7244449322332886E-4
23	1.0902565228230667E-4	3.634188409410222E-5	0.0	0.00719569305063224	6.5415391369384E-4
24	1.0902565228230667E-4	3.634188409410222E-5	0.0	0.009376206096278372	6.904957977879422E-4
25	1.0902565228230667E-4	3.634188409410222E-5	0.0	0.010611830155477849	6.904957977879422E-4
26	1.0902565228230667E-4	3.634188409410222E-5	0.0	0.012828685085218084	6.904957977879422E-4
27	1.0902565228230667E-4	3.634188409410222E-5	0.0	0.02256831002243748	6.904957977879422E-4
28	1.0902565228230667E-4	3.634188409410222E-5	0.0	0.06846810963328859	6.904957977879422E-4
29	1.0902565228230667E-4	3.634188409410222E-5	0.0	0.1705161201695276	6.904957977879422E-4
30	1.0902565228230667E-4	3.634188409410222E-5	0.0	0.29756734696250897	6.904957977879422E-4
31	1.0902565228230667E-4	3.634188409410222E-5	0.0	0.7272011007229855	6.904957977879422E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTATAC	2130	0.0	17.71831	37
GTACTAA	90	8.2791847E-4	14.388888	1
TCTTATA	3645	0.0	14.363511	37
GTTCTAG	325	0.0	14.230769	1
TATACAC	810	0.0	14.160494	37
TATACTG	410	0.0	13.987804	5
GTACTAG	210	2.044544E-9	13.214285	1
TATACTA	210	2.044544E-9	13.214285	5
TACTTAC	450	0.0	13.155556	31
AGTGTAC	665	0.0	13.075188	27
CGATAGA	85	0.009409084	13.058825	25
GTACTTA	475	0.0	12.852632	30
GTCTAGA	250	1.6007107E-10	12.58	1
CTCTTAT	5505	0.0	12.568574	37
TCGTTAC	265	3.274181E-11	12.566038	23
GTCTAAG	415	0.0	12.481928	1
TTTATAC	430	0.0	12.476744	3
AACGCAG	10465	0.0	12.445294	5
GTATTAG	240	1.1295924E-9	12.333334	1
GGACCGT	165	3.8083926E-6	12.333334	6
>>END_MODULE