FastQCFastQC Report
Thu 9 Feb 2017
SRR2727362.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727362.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9119580
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT426760.4679601472874847No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT162300.17796872224378754No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT143940.15783621614153284No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT119280.13079549716105346No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC54800.020.35675237
TCTTATA94650.015.53882737
AACGCAG201900.014.5690945
ACGCAGA230650.012.7129856
CGCAGAG232950.012.6271737
CTCTTAT148550.012.51598737
AGAGTAC232550.012.50569810
GAGTACT131950.012.4221311
TATACTG12250.012.3836745
CAGAGTA241050.012.3563589
CGTTACG3750.012.33333324
TATTCCG4400.012.1931825
AGTACTT136200.012.03450912
GTGCGAT4000.012.02500111
CAACGCA248850.011.8352414
GCAGAGT250550.011.8066258
GTCTTAG12700.011.79921151
TCAACGC249550.011.7798033
GGGTATA5500.011.7727271
ATCAACG250100.011.6873262
Produced by FastQC (version 0.11.5)