##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727362.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	9119580
Sequences flagged as poor quality	0
Sequence length	43
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.93094035032315	33.0	31.0	34.0	30.0	34.0
2	32.09006840227291	33.0	31.0	34.0	30.0	34.0
3	32.17192239116275	34.0	31.0	34.0	30.0	34.0
4	35.79594981347825	37.0	35.0	37.0	35.0	37.0
5	35.64413547553725	37.0	35.0	37.0	33.0	37.0
6	35.65978959557348	37.0	35.0	37.0	33.0	37.0
7	35.60831869450128	37.0	35.0	37.0	33.0	37.0
8	35.57637226714388	37.0	35.0	37.0	33.0	37.0
9	37.27157500674373	39.0	37.0	39.0	34.0	39.0
10	37.08390956601072	39.0	37.0	39.0	33.0	39.0
11	37.19605683595078	39.0	37.0	39.0	34.0	39.0
12	37.095768884093346	39.0	37.0	39.0	33.0	39.0
13	37.16063886714081	39.0	37.0	39.0	33.0	39.0
14	38.28421604942333	40.0	38.0	41.0	34.0	41.0
15	38.23437395143198	40.0	38.0	41.0	33.0	41.0
16	38.16885448671978	40.0	38.0	41.0	33.0	41.0
17	38.11842288789615	40.0	38.0	41.0	33.0	41.0
18	38.071024213834406	40.0	37.0	41.0	33.0	41.0
19	38.073641549281874	40.0	37.0	41.0	33.0	41.0
20	37.95793216354262	40.0	37.0	41.0	33.0	41.0
21	37.87363639553576	40.0	37.0	41.0	33.0	41.0
22	37.79489636584141	40.0	37.0	41.0	33.0	41.0
23	37.680731897740905	39.0	37.0	41.0	32.0	41.0
24	37.571482787584515	39.0	37.0	41.0	32.0	41.0
25	37.48900848503988	39.0	37.0	41.0	32.0	41.0
26	37.1198623182208	39.0	36.0	40.0	31.0	41.0
27	36.898115702696835	39.0	36.0	40.0	31.0	41.0
28	36.752762188609566	39.0	36.0	40.0	30.0	41.0
29	36.706047975893625	39.0	36.0	40.0	30.0	41.0
30	36.62976167762112	39.0	36.0	40.0	30.0	41.0
31	36.42085578502519	39.0	35.0	40.0	30.0	41.0
32	36.31679167242351	39.0	35.0	40.0	30.0	41.0
33	36.13610188188491	38.0	35.0	40.0	29.0	41.0
34	35.99875049070242	38.0	35.0	40.0	29.0	41.0
35	35.84393733044723	38.0	35.0	40.0	27.0	41.0
36	35.69489022520774	38.0	35.0	40.0	27.0	41.0
37	35.449329903350815	38.0	34.0	40.0	26.0	41.0
38	35.34187934093456	38.0	34.0	40.0	26.0	41.0
39	35.1759573357545	38.0	34.0	40.0	25.0	41.0
40	35.03984931323592	38.0	34.0	40.0	25.0	41.0
41	34.90685864919218	38.0	34.0	40.0	24.0	41.0
42	34.70623296248292	38.0	33.0	40.0	24.0	41.0
43	31.351349623557226	35.0	28.0	38.0	14.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	12.0
10	32.0
11	44.0
12	39.0
13	49.0
14	60.0
15	130.0
16	261.0
17	502.0
18	1012.0
19	2235.0
20	4438.0
21	8674.0
22	15438.0
23	24656.0
24	38112.0
25	54304.0
26	73872.0
27	98656.0
28	128630.0
29	163877.0
30	206242.0
31	257271.0
32	319132.0
33	400285.0
34	514213.0
35	675549.0
36	947720.0
37	1483756.0
38	2459970.0
39	1240409.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.93559352514041	19.06035146355424	12.81958160353876	26.184473407766585
2	18.67345864612186	21.312110864754736	34.49287138223471	25.5215591068887
3	18.800196938894114	22.23287695266668	30.649470699308523	28.31745540913068
4	14.976369525789565	15.884514418427164	33.36252327409815	35.776592781685125
5	14.525965011546585	37.056454354257546	34.059605815180085	14.357974819015787
6	36.39471335302723	35.41458049603162	14.001598757837533	14.189107393103631
7	30.21125972906647	30.954517642259844	20.011700100223916	18.822522528449774
8	28.100208562236418	35.35980823678283	18.465006063875748	18.074977137104998
9	26.873573125078128	13.745216336717261	18.046543810131606	41.334666728073
10	15.630807559120047	27.98989646453017	33.68714348687111	22.69215248947868
11	37.50734134686027	21.055410446533724	20.95224780088557	20.485000405720438
12	21.671568208185025	25.13264865267918	29.91283589814443	23.282947240991362
13	31.89183054482772	19.612021606258185	23.534307501003333	24.961840347910762
14	22.494435050736985	20.00213825636707	25.53369782380329	31.969728869092656
15	25.581901798109126	28.156329567809045	21.430285166641447	24.83148346744039
16	25.114785987951198	26.17698402777321	23.667109669524255	25.041120314751335
17	24.511600314926785	26.412981738194084	25.078325975538345	23.997091971340787
18	24.6206952513164	25.597724895225436	25.410676807484556	24.37090304597361
19	25.336572517594014	25.659229920676175	25.502885001282955	23.501312560446863
20	25.152156129997216	25.457575897135616	25.20847451308065	24.181793459786523
21	24.81962985137473	25.614425225723114	25.1957875253027	24.370157397599453
22	25.323457878542655	25.30884097732571	24.81066014005031	24.557041004081327
23	24.879314617559142	25.203430421137817	24.955699714241224	24.961555247061817
24	24.821143078957583	25.212487855800376	25.00346507185638	24.96290399338566
25	24.965031284335463	24.92716769851243	25.141728018176273	24.96607299897583
26	24.64983036499488	25.16926218093377	25.348820888681278	24.832086565390075
27	24.905675480669064	25.470964671618653	25.0696961921492	24.553663655563085
28	24.919513837260048	25.502567004182207	25.103754778180576	24.474164380377168
29	24.719131802122465	25.709374773838267	25.23038341678016	24.341110007259108
30	24.833150210864975	25.591507503635036	25.015757304612713	24.559584980887276
31	24.75614008539867	25.117340930174414	25.151750409558332	24.97476857486858
32	24.404369499472566	25.45589818829376	25.186697194388337	24.95303511784534
33	24.587963480774334	25.093085427179762	25.24245634119115	25.076494750854756
34	24.738737968195903	24.931126214145827	25.575256755245306	24.754879062412964
35	24.489406310378328	24.974889194458516	25.661357211625972	24.87434728353718
36	24.387340206456877	24.62879869467673	25.93937440101408	25.044486697852314
37	24.140837626294193	24.241489191388197	26.283129266917992	25.334543915399614
38	23.74345090453727	24.293037617960476	26.691020858416724	25.27249061908553
39	23.61703060886576	24.123545163264097	27.14400224571746	25.115421982152686
40	23.714951785060276	24.008528901550292	27.44392833880508	24.832590974584356
41	23.37173422460245	23.904927639211454	27.69933483778858	25.024003298397513
42	22.946297965476482	24.08894927178664	27.71686853999855	25.247884222738325
43	23.10102000311418	23.341754773794406	27.67159233210301	25.885632890988404
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	991.0
1	1063.5
2	1136.0
3	2554.0
4	3972.0
5	3972.0
6	4599.0
7	5226.0
8	5685.5
9	6145.0
10	9115.5
11	12086.0
12	12086.0
13	22474.5
14	32863.0
15	51388.5
16	69914.0
17	54521.0
18	39128.0
19	39128.0
20	46788.5
21	54449.0
22	51497.5
23	48546.0
24	59732.5
25	70919.0
26	70919.0
27	87609.0
28	104299.0
29	120923.5
30	137548.0
31	162563.5
32	187579.0
33	187579.0
34	218546.5
35	249514.0
36	287065.5
37	324617.0
38	369407.0
39	414197.0
40	414197.0
41	462653.0
42	511109.0
43	559854.5
44	608600.0
45	654277.0
46	699954.0
47	699954.0
48	741133.5
49	782313.0
50	802341.5
51	822370.0
52	818369.0
53	814368.0
54	814368.0
55	780356.0
56	746344.0
57	694550.0
58	642756.0
59	590105.5
60	537455.0
61	537455.0
62	469515.0
63	401575.0
64	341199.0
65	280823.0
66	233019.0
67	185215.0
68	185215.0
69	152604.5
70	119994.0
71	99329.0
72	78664.0
73	63764.0
74	48864.0
75	48864.0
76	39703.0
77	30542.0
78	24859.0
79	19176.0
80	15403.5
81	11631.0
82	11631.0
83	9234.0
84	6837.0
85	5302.0
86	3767.0
87	3022.5
88	2278.0
89	2278.0
90	1657.0
91	1036.0
92	757.5
93	479.0
94	352.5
95	226.0
96	226.0
97	140.5
98	55.0
99	37.5
100	20.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	9119580.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.935488375120045
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.2057822308874	42.72002546756317
2	10.362947632966952	11.178612831797336
3	3.7768457207320583	6.1111805539549735
4	1.809464051186124	3.9037730919178717
5	1.1359055352621894	3.063280989618416
6	0.7254310250312545	2.3475885970514785
7	0.5031160159193264	1.899506561956748
8	0.37865404611163594	1.6338312721797046
9	0.2767787423418959	1.3435376976055504
>10	1.6641714466063118	16.685826225264947
>50	0.12232887334346983	4.508110634379058
>100	0.037699835329692115	3.2039402255875786
>500	4.6813144992092814E-4	0.16422876826468683
>1k	3.2537031333147644E-4	0.30176605462126765
>5k	0.0	0.0
>10k+	8.134251838344578E-5	0.934791028237246
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	42676	0.4679601472874847	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	16230	0.17796872224378754	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	14394	0.15783621614153284	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	11928	0.13079549716105346	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	3.289625180106979E-5
2	0.0	1.0965417267023262E-5	0.0	1.0965417267023262E-5	3.289625180106979E-5
3	0.0	1.0965417267023262E-5	0.0	1.0965417267023262E-5	3.289625180106979E-5
4	0.0	1.0965417267023262E-5	0.0	1.0965417267023262E-5	3.289625180106979E-5
5	0.0	1.0965417267023262E-5	0.0	1.0965417267023262E-5	3.289625180106979E-5
6	0.0	1.0965417267023262E-5	0.0	1.0965417267023262E-5	7.675792086916284E-5
7	0.0	1.0965417267023262E-5	0.0	1.0965417267023262E-5	7.675792086916284E-5
8	0.0	1.0965417267023262E-5	0.0	1.0965417267023262E-5	7.675792086916284E-5
9	1.0965417267023262E-5	1.0965417267023262E-5	0.0	2.1930834534046524E-5	1.0965417267023262E-4
10	1.0965417267023262E-5	1.0965417267023262E-5	0.0	3.289625180106979E-5	1.0965417267023262E-4
11	1.0965417267023262E-5	1.0965417267023262E-5	0.0	5.482708633511631E-5	1.0965417267023262E-4
12	1.0965417267023262E-5	1.0965417267023262E-5	0.0	7.675792086916284E-5	1.2061958993725588E-4
13	1.0965417267023262E-5	2.1930834534046524E-5	0.0	9.868875540320936E-5	1.2061958993725588E-4
14	2.1930834534046524E-5	2.1930834534046524E-5	0.0	9.868875540320936E-5	1.3158500720427915E-4
15	2.1930834534046524E-5	2.1930834534046524E-5	0.0	1.5351584173832567E-4	1.3158500720427915E-4
16	3.289625180106979E-5	2.1930834534046524E-5	0.0	2.302737626074885E-4	1.425504244713024E-4
17	4.386166906809305E-5	2.1930834534046524E-5	0.0	3.728241870787909E-4	1.425504244713024E-4
18	4.386166906809305E-5	2.1930834534046524E-5	0.0	4.386166906809305E-4	1.6448125900534892E-4
19	4.386166906809305E-5	2.1930834534046524E-5	0.0	5.153746115500933E-4	1.754466762723722E-4
20	5.482708633511631E-5	2.1930834534046524E-5	0.0	7.456483741575818E-4	1.754466762723722E-4
21	6.579250360213957E-5	2.1930834534046524E-5	0.0	0.0010746108921682797	1.8641209353939544E-4
22	6.579250360213957E-5	2.1930834534046524E-5	0.0	0.0017106050936556288	1.8641209353939544E-4
23	6.579250360213957E-5	2.1930834534046524E-5	0.0	0.0021272909498025127	2.302737626074885E-4
24	7.675792086916284E-5	2.1930834534046524E-5	0.0	0.00302645516569842	2.302737626074885E-4
25	7.675792086916284E-5	2.1930834534046524E-5	0.0	0.0034541064391123274	2.52204597141535E-4
26	7.675792086916284E-5	2.1930834534046524E-5	0.0	0.004079135223332654	2.7413543167558157E-4
27	7.675792086916284E-5	2.1930834534046524E-5	0.0	0.008344682540204703	2.7413543167558157E-4
28	7.675792086916284E-5	2.1930834534046524E-5	0.0	0.03536347068615002	2.851008489426048E-4
29	7.675792086916284E-5	2.1930834534046524E-5	0.0	0.09274549924448275	2.851008489426048E-4
30	7.675792086916284E-5	2.1930834534046524E-5	0.0	0.17073154684755218	2.851008489426048E-4
31	7.675792086916284E-5	2.1930834534046524E-5	0.0	0.4796821783459326	2.851008489426048E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTATAC	5480	0.0	20.356752	37
TCTTATA	9465	0.0	15.538827	37
AACGCAG	20190	0.0	14.569094	5
ACGCAGA	23065	0.0	12.712985	6
CGCAGAG	23295	0.0	12.627173	7
CTCTTAT	14855	0.0	12.515987	37
AGAGTAC	23255	0.0	12.505698	10
GAGTACT	13195	0.0	12.42213	11
TATACTG	1225	0.0	12.383674	5
CAGAGTA	24105	0.0	12.356358	9
CGTTACG	375	0.0	12.333333	24
TATTCCG	440	0.0	12.193182	5
AGTACTT	13620	0.0	12.034509	12
GTGCGAT	400	0.0	12.025001	11
CAACGCA	24885	0.0	11.835241	4
GCAGAGT	25055	0.0	11.806625	8
GTCTTAG	1270	0.0	11.7992115	1
TCAACGC	24955	0.0	11.779803	3
GGGTATA	550	0.0	11.772727	1
ATCAACG	25010	0.0	11.687326	2
>>END_MODULE