FastQCFastQC Report
Thu 9 Feb 2017
SRR2727361.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727361.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3224128
Sequences flagged as poor quality0
Sequence length43
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT36410.11292975961252158No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCGT2150.017.2093036
CTTATAC12400.016.85887137
TCTTATA20250.015.43950737
CGTATAC750.004106542614.83
GTATTAC1904.5656634E-1014.6052641
CTCTTAT33650.014.56909337
TAGATCG1000.001833727512.9500015
GTAATAC2204.092726E-912.6136363
TATACTG3400.012.5147065
ACGTTCG1205.1258935E-412.3333347
ACCGTAT1205.1258935E-412.3333348
GTATAGA4150.012.0361441
GTCTTAG3700.012.01
TAAGACA5450.011.88073354
TGTACCG4550.011.7912085
TATACAG5050.011.7227725
TAAACAG5700.011.6842115
CGCGCAA1901.435943E-611.68421120
ATAAGAC2705.802576E-1011.6481493
TAAGAGT5100.011.6078434
Produced by FastQC (version 0.11.5)