##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727361.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3224128
Sequences flagged as poor quality	0
Sequence length	43
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.362614015324453	31.0	31.0	34.0	30.0	34.0
2	31.546741630605236	31.0	31.0	34.0	30.0	34.0
3	31.613120508863172	31.0	31.0	34.0	30.0	34.0
4	35.38843091837545	37.0	35.0	37.0	33.0	37.0
5	34.96954029120432	35.0	35.0	37.0	32.0	37.0
6	34.96082382585307	36.0	35.0	37.0	32.0	37.0
7	34.926949550390056	35.0	35.0	37.0	32.0	37.0
8	34.85457494243405	35.0	35.0	37.0	32.0	37.0
9	36.4657311992576	38.0	35.0	39.0	32.0	39.0
10	36.15243253369593	37.0	35.0	39.0	32.0	39.0
11	36.30166079014233	38.0	35.0	39.0	32.0	39.0
12	36.17307346358457	37.0	35.0	39.0	32.0	39.0
13	36.26701297218969	38.0	35.0	39.0	32.0	39.0
14	37.20927177829168	39.0	36.0	40.0	32.0	41.0
15	37.121437486352896	38.0	36.0	40.0	32.0	41.0
16	37.02811116680231	38.0	36.0	40.0	32.0	41.0
17	36.9362007339659	38.0	36.0	40.0	31.0	41.0
18	36.85586831540187	38.0	36.0	40.0	31.0	41.0
19	36.832769666712984	38.0	36.0	40.0	31.0	41.0
20	36.688079381463766	38.0	36.0	40.0	31.0	41.0
21	36.573218867241	38.0	36.0	40.0	30.0	41.0
22	36.47634244049864	38.0	35.0	40.0	30.0	41.0
23	36.332419494511385	38.0	35.0	40.0	30.0	41.0
24	36.182753290191954	38.0	35.0	40.0	30.0	41.0
25	36.07882069198245	38.0	35.0	40.0	29.0	41.0
26	35.63676318061814	38.0	34.0	40.0	27.0	41.0
27	35.35019825515612	38.0	34.0	40.0	27.0	41.0
28	35.16537556821565	38.0	34.0	40.0	26.0	41.0
29	35.097764728943766	38.0	34.0	40.0	26.0	41.0
30	34.99700849345932	38.0	34.0	40.0	26.0	41.0
31	34.75261279949183	38.0	33.0	40.0	25.0	40.0
32	34.630175042678204	38.0	33.0	40.0	25.0	40.0
33	34.428301233697916	38.0	33.0	40.0	24.0	40.0
34	34.257325701709114	37.0	33.0	39.0	24.0	40.0
35	34.068433077098675	37.0	32.0	39.0	24.0	40.0
36	33.890906626535916	37.0	32.0	39.0	23.0	40.0
37	33.5850896738591	37.0	31.0	39.0	22.0	40.0
38	33.445912817357126	37.0	31.0	39.0	21.0	40.0
39	33.24717939238145	37.0	31.0	39.0	20.0	40.0
40	33.09444693262798	37.0	31.0	39.0	19.0	40.0
41	32.930972963852554	36.0	30.0	39.0	19.0	40.0
42	32.690611538995974	36.0	30.0	39.0	18.0	40.0
43	29.460264915040593	33.0	24.0	38.0	9.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	7.0
10	6.0
11	11.0
12	28.0
13	41.0
14	76.0
15	152.0
16	294.0
17	597.0
18	1225.0
19	2216.0
20	4162.0
21	7156.0
22	11628.0
23	17443.0
24	24877.0
25	34048.0
26	44785.0
27	57394.0
28	71922.0
29	89781.0
30	109973.0
31	134228.0
32	163451.0
33	201764.0
34	255513.0
35	334514.0
36	453075.0
37	593448.0
38	521467.0
39	88845.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.47927749766759	19.471528425670446	12.75178901085813	26.29740506580384
2	20.016512992040017	21.493687595529707	33.02772098378228	25.462078428647995
3	19.585977976060505	22.238509141076285	29.870805377453998	28.304707505409215
4	16.12451490759672	16.15425938424281	32.19865960656649	35.52256610159398
5	15.192666047998094	36.54054677729916	33.451029239533916	14.815757935168827
6	37.26855757587788	34.720023522639295	13.521702612303233	14.489716289179585
7	30.75073322150982	30.487902465410805	19.456578647001606	19.304785666077773
8	28.341430613176648	34.678585961847666	18.141308285527124	18.838675139448558
9	27.117750908152534	13.596265408817516	17.445089028723427	41.840894654306524
10	16.092568285130117	27.55185278003851	33.05594566965083	23.29963326518054
11	38.34298762332017	20.53668464775592	20.129256654822637	20.991071074101274
12	22.30792325862993	24.751932925739922	28.899224844671178	24.04091897095897
13	32.71427188994978	19.246196180796794	22.479721648768287	25.55981028048514
14	23.059506322329636	19.911988605911425	24.303160420429958	32.72534465132898
15	26.624098050697736	27.497171328185484	20.468790320979814	25.409940300136967
16	26.028712259562898	25.680276961708714	22.77006371955456	25.52094705917383
17	25.618306717351174	25.898506510907755	23.768845405641464	24.71434136609961
18	25.594920549060085	25.091993866248487	24.04699813406912	25.266087450622308
19	26.365640570101434	25.2996469122814	24.052767135796095	24.281945381821068
20	26.196633632411615	24.92490992913433	23.696701867915916	25.181754570538146
21	25.729654653909524	25.11485276018818	23.75640793417631	25.399084651725985
22	26.335275770689005	24.885364352780037	23.243804216209778	25.53555566032118
23	25.797673045238902	24.637731504456397	23.38337683863668	26.181218611668022
24	25.786538251583064	24.837444419080136	23.441035839768148	25.934981489568653
25	26.077004386922603	24.286473738015363	23.581725043174465	26.05479683188757
26	25.76709113285825	24.589718522341546	23.86595693471227	25.777233410087934
27	26.184072096393194	24.808816523413462	23.491561129086687	25.515550251106657
28	25.861163080373977	25.003349742938248	23.632808622982708	25.502678553705067
29	25.93994407169939	25.048168062806443	23.654519919804674	25.357367945689504
30	25.859364144351588	24.983778559660163	23.644470690989934	25.512386604998312
31	25.991647974273974	24.355019403696133	23.696050529011256	25.95728209301864
32	25.544767453401352	24.715830140738827	23.69608154514957	26.043320860710246
33	25.817430325346884	24.470182325267483	23.63150594517339	26.080881404212242
34	26.071142336780674	24.4075917581436	23.77098551918534	25.750280385890385
35	25.722179764575102	24.591734571332154	23.984190453977014	25.701895210115726
36	25.642127111578695	24.349622595628958	24.091971534628897	25.916278758163447
37	25.32092398316692	24.020479335808005	24.44878118982869	26.20981549119638
38	25.111596065664887	23.974296305853862	24.818989816781468	26.095117811699787
39	24.899383647299363	23.81226799928539	25.096087996506345	26.192260356908907
40	25.133648540008334	23.606724050658038	25.545573872997597	25.714053536336028
41	25.030302767135797	23.369977866883698	25.63238804414713	25.967331321833377
42	24.43994159040832	23.496368630525836	25.814918018143203	26.248771760922647
43	25.079339281815116	22.686940468864762	25.39303650475415	26.840683744565975
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	109.0
1	107.0
2	105.0
3	175.5
4	246.0
5	246.0
6	348.5
7	451.0
8	528.5
9	606.0
10	905.5
11	1205.0
12	1205.0
13	2278.0
14	3351.0
15	5239.0
16	7127.0
17	6087.5
18	5048.0
19	5048.0
20	6632.5
21	8217.0
22	8996.0
23	9775.0
24	12642.5
25	15510.0
26	15510.0
27	20072.5
28	24635.0
29	29967.5
30	35300.0
31	43749.0
32	52198.0
33	52198.0
34	62607.0
35	73016.0
36	86187.0
37	99358.0
38	115612.0
39	131866.0
40	131866.0
41	150307.0
42	168748.0
43	187074.0
44	205400.0
45	225029.5
46	244659.0
47	244659.0
48	263825.0
49	282991.0
50	294937.0
51	306883.0
52	309538.5
53	312194.0
54	312194.0
55	300625.0
56	289056.0
57	269312.5
58	249569.0
59	228041.5
60	206514.0
61	206514.0
62	181872.0
63	157230.0
64	135306.0
65	113382.0
66	94880.0
67	76378.0
68	76378.0
69	63239.0
70	50100.0
71	41618.5
72	33137.0
73	27629.5
74	22122.0
75	22122.0
76	18547.5
77	14973.0
78	12191.0
79	9409.0
80	7513.0
81	5617.0
82	5617.0
83	4517.0
84	3417.0
85	2688.5
86	1960.0
87	1585.0
88	1210.0
89	1210.0
90	932.5
91	655.0
92	462.0
93	269.0
94	177.0
95	85.0
96	85.0
97	60.5
98	36.0
99	23.5
100	11.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	3224128.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	63.536314350993614
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.63853139336817	53.14084022464487
2	8.689060364680538	11.041417414902039
3	2.818099793407029	5.371550230391375
4	1.3859859091377984	3.5224174563610733
5	0.8052232972520883	2.5580460268476126
6	0.5250765919342422	2.001685884208944
7	0.3691307577060051	1.641724550076044
8	0.27238267270108407	1.3844952893199909
9	0.21148323778144915	1.2093178928083275
>10	1.2154671294789319	13.661301913785387
>50	0.05384954836924845	2.2861348316067303
>100	0.01492730947270252	1.5647250942029476
>500	3.421226862767477E-4	0.15936483567729848
>1k	4.3987202441375965E-4	0.4569783551674202
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	3641	0.11292975961252158	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	9.304841495126745E-5
2	0.0	0.0	0.0	0.0	9.304841495126745E-5
3	0.0	0.0	0.0	0.0	9.304841495126745E-5
4	0.0	0.0	0.0	0.0	1.240645532683566E-4
5	0.0	0.0	0.0	0.0	1.240645532683566E-4
6	0.0	0.0	0.0	0.0	1.860968299025349E-4
7	0.0	0.0	0.0	0.0	1.860968299025349E-4
8	6.20322766341783E-5	0.0	0.0	0.0	1.860968299025349E-4
9	9.304841495126745E-5	0.0	0.0	0.0	1.860968299025349E-4
10	1.860968299025349E-4	0.0	0.0	0.0	1.860968299025349E-4
11	1.860968299025349E-4	0.0	0.0	0.0	1.860968299025349E-4
12	1.860968299025349E-4	0.0	0.0	0.0	1.860968299025349E-4
13	1.860968299025349E-4	0.0	0.0	0.0	1.860968299025349E-4
14	1.860968299025349E-4	0.0	0.0	0.0	3.4117752148798064E-4
15	1.860968299025349E-4	0.0	0.0	0.0	3.4117752148798064E-4
16	1.860968299025349E-4	0.0	0.0	0.0	3.4117752148798064E-4
17	1.860968299025349E-4	0.0	0.0	0.0	3.4117752148798064E-4
18	1.860968299025349E-4	0.0	0.0	6.20322766341783E-5	4.342259364392481E-4
19	1.860968299025349E-4	0.0	0.0	1.5508069158544574E-4	4.342259364392481E-4
20	1.860968299025349E-4	0.0	0.0	2.481291065367132E-4	4.342259364392481E-4
21	1.860968299025349E-4	0.0	0.0	3.101613831708915E-4	4.342259364392481E-4
22	2.1711296821962404E-4	0.0	0.0	6.513389046588721E-4	4.342259364392481E-4
23	2.1711296821962404E-4	0.0	0.0	7.443873196101396E-4	5.893066280246938E-4
24	2.1711296821962404E-4	0.0	0.0	0.0011786132560493877	5.893066280246938E-4
25	2.1711296821962404E-4	3.101613831708915E-5	0.0	0.0012096293943664768	6.20322766341783E-4
26	2.1711296821962404E-4	3.101613831708915E-5	0.0	0.0017058876074399032	6.20322766341783E-4
27	2.1711296821962404E-4	3.101613831708915E-5	0.0	0.0040010818429045	6.20322766341783E-4
28	2.1711296821962404E-4	3.101613831708915E-5	0.0	0.016996843797764852	6.20322766341783E-4
29	2.1711296821962404E-4	3.101613831708915E-5	0.0	0.04965683744565973	6.513389046588721E-4
30	2.1711296821962404E-4	3.101613831708915E-5	0.0	0.09314146336621872	6.513389046588721E-4
31	2.1711296821962404E-4	3.101613831708915E-5	0.0	0.25718581892530323	6.513389046588721E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTACCGT	215	0.0	17.209303	6
CTTATAC	1240	0.0	16.858871	37
TCTTATA	2025	0.0	15.439507	37
CGTATAC	75	0.0041065426	14.8	3
GTATTAC	190	4.5656634E-10	14.605264	1
CTCTTAT	3365	0.0	14.569093	37
TAGATCG	100	0.0018337275	12.950001	5
GTAATAC	220	4.092726E-9	12.613636	3
TATACTG	340	0.0	12.514706	5
ACGTTCG	120	5.1258935E-4	12.333334	7
ACCGTAT	120	5.1258935E-4	12.333334	8
GTATAGA	415	0.0	12.036144	1
GTCTTAG	370	0.0	12.0	1
TAAGACA	545	0.0	11.8807335	4
TGTACCG	455	0.0	11.791208	5
TATACAG	505	0.0	11.722772	5
TAAACAG	570	0.0	11.684211	5
CGCGCAA	190	1.435943E-6	11.684211	20
ATAAGAC	270	5.802576E-10	11.648149	3
TAAGAGT	510	0.0	11.607843	4
>>END_MODULE