##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727360.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1828721
Sequences flagged as poor quality	0
Sequence length	43
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.506707693519132	31.0	31.0	34.0	30.0	34.0
2	31.677996260774606	31.0	31.0	34.0	30.0	34.0
3	31.7408565877463	31.0	31.0	34.0	30.0	34.0
4	35.484659496992705	37.0	35.0	37.0	33.0	37.0
5	35.160161118071045	37.0	35.0	37.0	32.0	37.0
6	35.15551032661625	37.0	35.0	37.0	32.0	37.0
7	35.1049668046684	36.0	35.0	37.0	32.0	37.0
8	35.038083994223285	36.0	35.0	37.0	32.0	37.0
9	36.68687186290309	38.0	35.0	39.0	32.0	39.0
10	36.387618997102344	38.0	35.0	39.0	32.0	39.0
11	36.53597404962266	38.0	35.0	39.0	32.0	39.0
12	36.38192594715104	38.0	35.0	39.0	32.0	39.0
13	36.47709519385406	38.0	35.0	39.0	32.0	39.0
14	37.46722381380211	39.0	36.0	40.0	32.0	41.0
15	37.38369713039879	39.0	36.0	40.0	32.0	41.0
16	37.280006080752614	39.0	36.0	40.0	32.0	41.0
17	37.1729055443668	39.0	36.0	40.0	32.0	41.0
18	37.075927383127336	39.0	36.0	40.0	31.0	41.0
19	37.031291268597016	39.0	36.0	40.0	31.0	41.0
20	36.87252894235917	39.0	36.0	40.0	31.0	41.0
21	36.743773380411774	38.0	36.0	40.0	31.0	41.0
22	36.63190120308128	38.0	36.0	40.0	30.0	41.0
23	36.476404547221804	38.0	35.0	40.0	30.0	41.0
24	36.31450396205873	38.0	35.0	40.0	30.0	41.0
25	36.177026457289	38.0	35.0	40.0	29.0	41.0
26	35.70349769046235	38.0	34.0	40.0	27.0	41.0
27	35.385242472744615	38.0	34.0	40.0	27.0	41.0
28	35.17724026792496	38.0	34.0	40.0	26.0	41.0
29	35.10619389179651	38.0	34.0	40.0	25.0	41.0
30	34.990410784367874	38.0	34.0	40.0	25.0	41.0
31	34.72183673726063	38.0	33.0	40.0	25.0	41.0
32	34.576718373114325	38.0	33.0	40.0	24.0	41.0
33	34.355310077371016	38.0	33.0	40.0	24.0	41.0
34	34.166148909538414	38.0	33.0	40.0	23.0	40.0
35	33.95588610837848	37.0	32.0	40.0	23.0	40.0
36	33.742249364446515	37.0	32.0	40.0	21.0	40.0
37	33.41801291722466	37.0	31.0	39.0	20.0	40.0
38	33.275529181323996	37.0	31.0	39.0	19.0	40.0
39	33.04189157340021	37.0	31.0	39.0	18.0	40.0
40	32.8729789836722	37.0	30.0	39.0	18.0	40.0
41	32.689840604444306	36.0	30.0	39.0	17.0	40.0
42	32.44463370847713	36.0	30.0	39.0	15.0	40.0
43	28.918797892078672	33.0	24.0	37.0	9.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	4.0
11	5.0
12	14.0
13	15.0
14	33.0
15	54.0
16	127.0
17	288.0
18	639.0
19	1233.0
20	2529.0
21	4376.0
22	7212.0
23	10934.0
24	15421.0
25	20611.0
26	27039.0
27	33765.0
28	42001.0
29	50791.0
30	62094.0
31	74346.0
32	89894.0
33	109091.0
34	135689.0
35	174615.0
36	236444.0
37	323581.0
38	336624.0
39	69250.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.78132093413922	17.538596647602343	11.556656264132146	28.123426154126296
2	20.951856516111533	20.075834421981263	31.087574321069205	27.884734740837995
3	19.844798632486857	20.54523352660138	28.536337691752873	31.073630149158895
4	16.796110505648485	15.039746358247102	30.32348838341114	37.840654752693275
5	15.96760796206748	35.74268573500277	32.59474791397922	15.694958388950528
6	39.75964622268788	33.00913589333748	12.593118359771665	14.63809952420298
7	32.83983724143814	29.239342688141058	17.964686794759835	19.956133275660967
8	30.093764986567113	33.23918738834409	16.995047358235617	19.672000266853175
9	27.978789547448734	12.382479339385286	15.664663991937536	43.974067121228444
10	16.589189931104855	26.48200572968758	31.808405984291753	25.120398354915814
11	40.92811314574503	19.100507950638725	18.6991345317301	21.272244371886142
12	23.71318533554326	23.12917060612308	27.309086514564	25.84855754376966
13	34.24852670254238	17.819011210567385	20.960824532555815	26.97163755433442
14	24.315956343258485	17.949156815063645	22.224330556711493	35.51055628496638
15	27.857994740586456	25.75712752245969	18.81435166982826	27.570526067125606
16	27.886265865596776	24.12128476678509	20.743951647080117	27.24849772053802
17	27.36333207744648	24.014488814860222	21.756845358039854	26.86533374965345
18	27.4064223028007	23.49417981201069	21.92603464388499	27.173363241303623
19	28.247720674722938	23.487891263894273	21.90645812018345	26.357929941199345
20	28.148306931456467	22.971902220185584	21.708396196029902	27.171394652328047
21	27.658073593511528	23.11462492091467	21.78992858943491	27.437372896138886
22	28.336471227705047	22.81299334343511	21.353612716209856	27.49692271264999
23	27.87625887163761	22.802002054988158	21.418029322132792	27.90370975124144
24	27.815506028530322	22.70089313788161	21.472767032259156	28.010833801328904
25	27.954236868281164	22.29716834880772	21.68548400767531	28.063110775235806
26	27.679235924998945	22.596011091905215	21.735847075633735	27.988905907462104
27	27.931926193224665	22.877355266330948	21.568079548493184	27.622638991951202
28	27.82026345188796	23.013844101970722	21.454830999370596	27.711061446770724
29	27.879649219317766	23.276431998101405	21.450511040229756	27.393407742351073
30	28.05157265651786	23.0082117501795	21.36361971016902	27.57659588313362
31	27.90928742000557	22.544116899188012	21.56518134805692	27.981414332749498
32	27.495938418162204	22.97671432657032	21.617184906828324	27.910162348439155
33	27.44841886761294	22.647850601595323	21.683515418699738	28.220215112092
34	27.62843539282373	22.550132032168932	22.060828305684684	27.76060426932266
35	27.50523453276908	22.49725354496394	22.219846548489354	27.777665373777626
36	27.359832363712123	22.415994566694426	22.288200332363438	27.93597273723001
37	27.13896761725818	22.025503070178555	22.664255509725102	28.171273802838158
38	26.855108023585885	22.022112722498402	22.894416370785923	28.228362883129794
39	26.527775423369665	21.896997956495277	23.441246641778598	28.13397997835646
40	26.836843892534727	21.501147523323677	23.79280382299979	27.869204761141802
41	26.448266301967333	21.406163105252247	24.128448243335097	28.017122349445323
42	26.025457136435794	21.26212801187278	24.47322472919598	28.23919012249545
43	26.38122491074363	20.17109225518819	24.191005626336658	29.256677207731524
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	18.0
1	15.0
2	12.0
3	27.5
4	43.0
5	43.0
6	54.5
7	66.0
8	78.0
9	90.0
10	118.5
11	147.0
12	147.0
13	266.5
14	386.0
15	649.0
16	912.0
17	803.0
18	694.0
19	694.0
20	951.0
21	1208.0
22	1510.5
23	1813.0
24	2369.0
25	2925.0
26	2925.0
27	3892.0
28	4859.0
29	6212.0
30	7565.0
31	9834.0
32	12103.0
33	12103.0
34	15205.5
35	18308.0
36	23063.5
37	27819.0
38	34507.5
39	41196.0
40	41196.0
41	50009.0
42	58822.0
43	70121.0
44	81420.0
45	95354.5
46	109289.0
47	109289.0
48	125297.5
49	141306.0
50	155870.0
51	170434.0
52	180033.0
53	189632.0
54	189632.0
55	192610.0
56	195588.0
57	190270.5
58	184953.0
59	174282.0
60	163611.0
61	163611.0
62	147141.0
63	130671.0
64	113613.5
65	96556.0
66	81316.0
67	66076.0
68	66076.0
69	54774.0
70	43472.0
71	36082.5
72	28693.0
73	23585.0
74	18477.0
75	18477.0
76	15169.5
77	11862.0
78	9691.5
79	7521.0
80	6061.5
81	4602.0
82	4602.0
83	3608.5
84	2615.0
85	2039.5
86	1464.0
87	1158.0
88	852.0
89	852.0
90	632.0
91	412.0
92	284.0
93	156.0
94	105.0
95	54.0
96	54.0
97	33.5
98	13.0
99	9.5
100	6.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1828721.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	70.35851720721415
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.77866988763151	59.649015040936504
2	8.483088804418804	11.937150992320523
3	2.7162614702300214	5.733363881774155
4	1.3066865179207647	3.6774610342225156
5	0.7266901362367505	2.5564420227363107
6	0.45511328425237557	1.921265750478151
7	0.30627068748339986	1.508412600475627
8	0.23000651497422442	1.2946333873268263
9	0.1716760618410575	1.0870985835999727
>10	0.8025296172930246	9.304637115488436
>50	0.018721900399272927	0.8784887754196988
>100	0.004207241829487884	0.42348416257735394
>500	7.787548941986646E-5	0.028546652643936753
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	5.468302709926774E-5
2	0.0	0.0	0.0	0.0	5.468302709926774E-5
3	0.0	0.0	0.0	0.0	5.468302709926774E-5
4	0.0	0.0	0.0	0.0	5.468302709926774E-5
5	0.0	0.0	0.0	0.0	5.468302709926774E-5
6	0.0	0.0	0.0	0.0	5.468302709926774E-5
7	0.0	0.0	0.0	0.0	1.0936605419853548E-4
8	0.0	0.0	0.0	0.0	1.0936605419853548E-4
9	0.0	0.0	0.0	0.0	2.734151354963387E-4
10	0.0	0.0	0.0	0.0	2.734151354963387E-4
11	0.0	0.0	0.0	0.0	2.734151354963387E-4
12	0.0	0.0	0.0	0.0	3.2809816259560644E-4
13	0.0	0.0	0.0	0.0	3.2809816259560644E-4
14	5.468302709926774E-5	0.0	0.0	0.0	4.374642167941419E-4
15	5.468302709926774E-5	0.0	0.0	5.468302709926774E-5	4.921472438934097E-4
16	5.468302709926774E-5	0.0	0.0	1.0936605419853548E-4	6.561963251912129E-4
17	5.468302709926774E-5	0.0	0.0	1.6404908129780322E-4	6.561963251912129E-4
18	5.468302709926774E-5	0.0	0.0	1.6404908129780322E-4	7.655623793897484E-4
19	5.468302709926774E-5	0.0	0.0	1.6404908129780322E-4	8.202454064890161E-4
20	5.468302709926774E-5	0.0	0.0	1.6404908129780322E-4	8.202454064890161E-4
21	5.468302709926774E-5	0.0	0.0	1.6404908129780322E-4	8.202454064890161E-4
22	5.468302709926774E-5	0.0	0.0	2.734151354963387E-4	8.202454064890161E-4
23	5.468302709926774E-5	0.0	0.0	2.734151354963387E-4	9.842944877868193E-4
24	5.468302709926774E-5	0.0	0.0	6.561963251912129E-4	9.842944877868193E-4
25	5.468302709926774E-5	0.0	0.0	7.655623793897484E-4	9.842944877868193E-4
26	5.468302709926774E-5	0.0	0.0	0.001038977514886087	9.842944877868193E-4
27	5.468302709926774E-5	0.0	0.0	0.003171615571757529	0.0010936605419853548
28	5.468302709926774E-5	0.0	0.0	0.018100081969857622	0.0010936605419853548
29	5.468302709926774E-5	0.0	0.0	0.05129267941911314	0.0010936605419853548
30	5.468302709926774E-5	0.0	0.0	0.09514846715272586	0.0010936605419853548
31	5.468302709926774E-5	5.468302709926774E-5	0.0	0.2327309633344835	0.0011483435690846225
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	775	0.0	20.529032	1
ATTATAG	100	2.877241E-7	18.5	3
ATATTAC	50	0.0070364336	18.5	1
TCGTTAC	160	3.45608E-11	17.34375	23
CGTTACG	150	4.6748028E-9	16.033333	24
CGAACCG	70	0.00259328	15.857142	18
TAAGTAG	70	0.00259328	15.857142	5
TACTACG	70	0.00259328	15.857142	2
TATACAT	105	9.3490635E-6	15.857142	5
GTATCAA	1010	0.0	15.569307	2
TTACGGA	250	0.0	15.54	26
TAAGACT	240	0.0	15.416667	4
CTCGTTA	170	1.4879333E-9	15.235294	22
TAACGCG	85	5.3663034E-4	15.235294	5
TAGACAG	270	0.0	15.074075	5
ATAAGAC	135	3.9755832E-7	15.074075	3
GTTACGG	210	9.094947E-12	14.97619	25
TAGGAGT	235	0.0	14.957447	4
ATTACGG	150	8.108691E-8	14.8	25
TTTTACG	75	0.00410564	14.8	2
>>END_MODULE