FastQCFastQC Report
Thu 9 Feb 2017
SRR2727359.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727359.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences863635
Sequences flagged as poor quality0
Sequence length43
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT32560.3770111215965078No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT13090.15156866037156902No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11180.1294528359781621No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTTTA358.867602E-426.42857226
CGCAATC652.681565E-622.76922822
GCAATCG705.0994986E-621.14285923
TAGGCCG450.003825048720.5555575
CTTATAC4050.019.64197537
AATCGGG902.150955E-618.525
ACGCGCA852.7218464E-517.41176419
AGATCGA650.001579504517.0769216
CGGTCGT1757.2759576E-1216.91428617
GGGTATA2100.016.7380941
TAGACCG803.3813468E-416.18755
GGTATAG2100.015.8571422
AACGACG1059.337642E-615.85714215
AATCGGT2055.456968E-1215.34146314
GGCTTAA855.3622335E-415.2352941
ATAGTGT855.3622335E-415.2352946
TATACTG855.3622335E-415.2352945
TCGTTAC1101.4509575E-515.13636423
CTAATCG2201.8189894E-1215.13636419
ACATTCG750.004103307614.88
Produced by FastQC (version 0.11.5)