##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727359.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	863635
Sequences flagged as poor quality	0
Sequence length	43
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.11118122818089	33.0	31.0	34.0	30.0	34.0
2	32.26511662913152	33.0	31.0	34.0	30.0	34.0
3	32.332391577460385	34.0	31.0	34.0	30.0	34.0
4	35.9146942863594	37.0	35.0	37.0	35.0	37.0
5	35.80314253127768	37.0	35.0	37.0	35.0	37.0
6	35.81649307867328	37.0	35.0	37.0	35.0	37.0
7	35.761112043861125	37.0	35.0	37.0	35.0	37.0
8	35.73042199540315	37.0	35.0	37.0	35.0	37.0
9	37.47198642945226	39.0	37.0	39.0	35.0	39.0
10	37.296772363324784	39.0	37.0	39.0	34.0	39.0
11	37.41102780688601	39.0	37.0	39.0	35.0	39.0
12	37.30691553723506	39.0	37.0	39.0	34.0	39.0
13	37.36511026070041	39.0	37.0	39.0	34.0	39.0
14	38.4927973044168	40.0	38.0	41.0	34.0	41.0
15	38.45726493252358	40.0	38.0	41.0	34.0	41.0
16	38.36409246961969	40.0	38.0	41.0	34.0	41.0
17	38.3237351427397	40.0	38.0	41.0	34.0	41.0
18	38.26539568220371	40.0	38.0	41.0	33.0	41.0
19	38.263973785221765	40.0	38.0	41.0	34.0	41.0
20	38.130393047989024	40.0	38.0	41.0	33.0	41.0
21	38.04001458949672	40.0	38.0	41.0	33.0	41.0
22	37.951828029202154	40.0	37.0	41.0	33.0	41.0
23	37.82807899170367	40.0	37.0	41.0	33.0	41.0
24	37.68080728548519	40.0	37.0	41.0	32.0	41.0
25	37.58052186398189	40.0	37.0	41.0	32.0	41.0
26	37.178175965541	39.0	37.0	41.0	31.0	41.0
27	36.94403422742246	39.0	36.0	41.0	30.0	41.0
28	36.74116495973415	39.0	36.0	40.0	30.0	41.0
29	36.68240634064159	39.0	36.0	40.0	30.0	41.0
30	36.58248449865973	39.0	36.0	40.0	30.0	41.0
31	36.352897925628305	39.0	35.0	40.0	30.0	41.0
32	36.21438107533854	39.0	35.0	40.0	29.0	41.0
33	36.01239991431565	39.0	35.0	40.0	27.0	41.0
34	35.82836267636212	38.0	35.0	40.0	27.0	41.0
35	35.651910818806556	38.0	35.0	40.0	26.0	41.0
36	35.4637039953221	38.0	35.0	40.0	25.0	41.0
37	35.21285033607948	38.0	34.0	40.0	25.0	41.0
38	35.06974821539192	38.0	34.0	40.0	24.0	41.0
39	34.88393476410752	38.0	34.0	40.0	24.0	41.0
40	34.73361663202626	38.0	33.0	40.0	23.0	41.0
41	34.56442362803731	38.0	33.0	40.0	23.0	41.0
42	34.34392885883504	38.0	33.0	40.0	22.0	41.0
43	30.906510273437274	35.0	27.0	38.0	11.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	3.0
11	5.0
12	7.0
13	6.0
14	9.0
15	26.0
16	42.0
17	82.0
18	158.0
19	316.0
20	655.0
21	1069.0
22	1871.0
23	2897.0
24	4291.0
25	5895.0
26	7755.0
27	10077.0
28	12520.0
29	15841.0
30	19170.0
31	23664.0
32	29394.0
33	36611.0
34	46444.0
35	60014.0
36	83321.0
37	127868.0
38	222900.0
39	150724.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.57377248490392	17.85036502689215	12.384861660307884	27.191000827896044
2	20.178547650338395	21.05634903634058	32.95616782552814	25.808935487792876
3	19.235440898064578	21.353117925975674	30.37023742669067	29.04120374926908
4	15.568845635019423	15.66506683957922	32.26895621414139	36.49713131125997
5	15.277866228209833	36.34336264741471	33.32194735044319	15.056823773932274
6	37.063805890219825	34.233211947176756	13.58583197762944	15.117150184973976
7	30.745974862065573	30.350668974740486	19.315914709339015	19.58744145385493
8	28.935950951501503	33.42511593439358	18.39585009871068	19.243083015394234
9	28.196865573998274	13.06859958199934	17.358258986724717	41.37627585727767
10	16.326457357564248	27.092579619862555	32.30531416628553	24.275648856287667
11	38.2650077868544	20.393569042477434	19.84055764298575	21.50086552768241
12	22.58766724368512	25.144071280112545	28.065560103515953	24.202701372686377
13	33.37717901659845	18.931377260069358	22.156813931811474	25.534629791520725
14	23.177499753946982	20.252074082222236	24.4082280129916	32.16219815083918
15	27.437632796262314	26.955832035524267	20.51700081631708	25.089534351896347
16	25.781493339200008	25.29042940594117	23.029057414301178	25.899019840557642
17	25.905619850978713	24.954870981375237	23.60233200368211	25.537177163963943
18	25.457050721659037	24.522280824653933	24.54265980419969	25.478008649487343
19	27.307601012001598	24.247627759412254	24.11748018549503	24.32729104309112
20	26.139978115754918	24.09837489217088	24.12894336148952	25.63270363058468
21	25.958188355034245	24.487312348387917	24.421543823490246	25.132955473087588
22	26.547673496326574	24.188343455279142	23.33682632130472	25.927156727089567
23	25.84019869505057	24.083438026481094	23.76443752279609	26.311925755672245
24	25.472103377005332	24.210806648642077	23.982816814973916	26.334273159378675
25	26.010293700463738	23.700290053089557	23.846300809948648	26.44311543649806
26	25.61869308214697	24.074869591899358	24.156385510082384	26.15005181587129
27	26.52115766498579	24.29290151510766	23.915774603854636	25.27016621605192
28	25.579903547216126	24.659259988305244	23.750542764014888	26.010293700463738
29	25.64740891696145	24.47399653788927	24.04024848460287	25.838346060546414
30	26.044799018103713	24.638649429446467	23.81827971307323	25.498271839376592
31	25.83383026394252	23.511321333665265	24.245775124908093	26.409073277484126
32	25.310924175143434	24.420501716581658	23.96475362855836	26.303820479716546
33	25.802451267028314	23.503795005992114	23.979343125278618	26.71441060170095
34	25.946956758352773	23.877216648236814	24.70349163709206	25.472334956318356
35	25.97555680351074	23.9733220631401	24.17363816890237	25.877482964446784
36	25.305713640600487	23.401089580667758	25.05896588257771	26.23423089615405
37	25.166650263131995	23.428184360291095	24.96691310565227	26.43825227092464
38	25.312313651021555	22.94522570298795	25.324934723581144	26.41752592240935
39	24.792418093291726	22.8912677230543	26.036346373178482	26.27996781047549
40	25.22836615005182	22.32771946482021	26.579631441523325	25.86428294360465
41	24.580523021878456	22.410624858881356	26.763736995374206	26.245115123865986
42	24.062016940026748	22.52004608428329	26.91576881437181	26.502168161318153
43	24.287690980564705	21.547644548912444	26.571294586254613	27.593369884268238
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	50.0
1	57.0
2	64.0
3	167.5
4	271.0
5	271.0
6	304.0
7	337.0
8	357.0
9	377.0
10	529.5
11	682.0
12	682.0
13	1419.5
14	2157.0
15	3516.0
16	4875.0
17	3649.0
18	2423.0
19	2423.0
20	2925.5
21	3428.0
22	3087.5
23	2747.0
24	3565.5
25	4384.0
26	4384.0
27	5613.0
28	6842.0
29	8008.5
30	9175.0
31	11044.0
32	12913.0
33	12913.0
34	15289.5
35	17666.0
36	20821.5
37	23977.0
38	28100.0
39	32223.0
40	32223.0
41	36503.5
42	40784.0
43	45741.0
44	50698.0
45	56223.5
46	61749.0
47	61749.0
48	68382.5
49	75016.0
50	78574.0
51	82132.0
52	82738.0
53	83344.0
54	83344.0
55	78572.0
56	73800.0
57	70514.5
58	67229.0
59	61999.0
60	56769.0
61	56769.0
62	50477.0
63	44185.0
64	37975.0
65	31765.0
66	26870.5
67	21976.0
68	21976.0
69	18455.5
70	14935.0
71	12924.0
72	10913.0
73	9361.0
74	7809.0
75	7809.0
76	6615.5
77	5422.0
78	4673.0
79	3924.0
80	3140.0
81	2356.0
82	2356.0
83	1987.5
84	1619.0
85	1305.5
86	992.0
87	933.0
88	874.0
89	874.0
90	652.0
91	430.0
92	312.0
93	194.0
94	144.5
95	95.0
96	95.0
97	58.0
98	21.0
99	17.0
100	13.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	863635.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	69.16208986246754
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.68728119647767	61.337977117687146
2	5.949578104401062	8.22970511000711
3	1.8531944477963014	3.84512402793341
4	0.9529085977188432	2.636206002645942
5	0.5596332768744795	1.9352703492609962
6	0.39573945838246904	1.6422100789663534
7	0.2701615279419129	1.3079455111030078
8	0.20989749350683665	1.1613559446261226
9	0.15623787248768517	0.9725163999222624
>10	0.8926460492273707	11.10552509679097
>50	0.049265556144667697	2.2815658067702866
>100	0.022283598126405322	2.5939337373960774
>500	6.701833796087911E-4	0.29212896189427506
>1k	5.026375347065934E-4	0.6585358549960989
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	3256	0.3770111215965078	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1309	0.15156866037156902	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1118	0.1294528359781621	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	1.1578965650998396E-4	0.0	0.0	0.0	0.0
11	1.1578965650998396E-4	0.0	0.0	0.0	0.0
12	1.1578965650998396E-4	0.0	0.0	1.1578965650998396E-4	0.0
13	1.1578965650998396E-4	0.0	0.0	1.1578965650998396E-4	0.0
14	1.1578965650998396E-4	0.0	0.0	1.1578965650998396E-4	0.0
15	1.1578965650998396E-4	0.0	0.0	1.1578965650998396E-4	0.0
16	1.1578965650998396E-4	0.0	0.0	1.1578965650998396E-4	1.1578965650998396E-4
17	1.1578965650998396E-4	0.0	0.0	1.1578965650998396E-4	1.1578965650998396E-4
18	1.1578965650998396E-4	0.0	0.0	3.473689695299519E-4	1.1578965650998396E-4
19	1.1578965650998396E-4	0.0	0.0	5.789482825499198E-4	1.1578965650998396E-4
20	1.1578965650998396E-4	0.0	0.0	8.105275955698877E-4	1.1578965650998396E-4
21	1.1578965650998396E-4	0.0	0.0	0.0015052655346297916	1.1578965650998396E-4
22	1.1578965650998396E-4	0.0	0.0	0.0032421103822795508	1.1578965650998396E-4
23	1.1578965650998396E-4	0.0	0.0	0.0037052690083194868	2.3157931301996792E-4
24	1.1578965650998396E-4	0.0	0.0	0.00497895522992931	2.3157931301996792E-4
25	1.1578965650998396E-4	0.0	0.0	0.005442113855969246	2.3157931301996792E-4
26	1.1578965650998396E-4	0.0	0.0	0.0064842207645591015	2.3157931301996792E-4
27	1.1578965650998396E-4	0.0	0.0	0.011463175994488413	2.3157931301996792E-4
28	1.1578965650998396E-4	0.0	0.0	0.03334742107487538	2.3157931301996792E-4
29	1.1578965650998396E-4	0.0	0.0	0.09402120108610698	2.3157931301996792E-4
30	1.1578965650998396E-4	0.0	0.0	0.16581078812229705	2.3157931301996792E-4
31	1.1578965650998396E-4	0.0	0.0	0.4220532979788916	2.3157931301996792E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGTTTA	35	8.867602E-4	26.428572	26
CGCAATC	65	2.681565E-6	22.769228	22
GCAATCG	70	5.0994986E-6	21.142859	23
TAGGCCG	45	0.0038250487	20.555557	5
CTTATAC	405	0.0	19.641975	37
AATCGGG	90	2.150955E-6	18.5	25
ACGCGCA	85	2.7218464E-5	17.411764	19
AGATCGA	65	0.0015795045	17.076921	6
CGGTCGT	175	7.2759576E-12	16.914286	17
GGGTATA	210	0.0	16.738094	1
TAGACCG	80	3.3813468E-4	16.1875	5
GGTATAG	210	0.0	15.857142	2
AACGACG	105	9.337642E-6	15.857142	15
AATCGGT	205	5.456968E-12	15.341463	14
GGCTTAA	85	5.3622335E-4	15.235294	1
ATAGTGT	85	5.3622335E-4	15.235294	6
TATACTG	85	5.3622335E-4	15.235294	5
TCGTTAC	110	1.4509575E-5	15.136364	23
CTAATCG	220	1.8189894E-12	15.136364	19
ACATTCG	75	0.0041033076	14.8	8
>>END_MODULE