FastQCFastQC Report
Thu 9 Feb 2017
SRR2727358.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727358.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2040052
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGT107140.5251826914215912No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT102780.503810687178562No Hit
CTTCCGTACTCTGCGTTGATACCACTGCTTCCGTACTCTGCGT82540.40459752986688574No Hit
GAGTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTG71520.35057929895904616No Hit
TCCGTACTCTGCGTTGATACCACTGCTTCCGTACTCTGCGTTG70190.3440598572977552No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT40200.19705380058939675No Hit
TATCAACGCAGAGTACGGAAGCAGTGGTATCAACGCAGAGTAC36800.18038755874850249No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35900.17597590649650108No Hit
GTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCTGTCTC32430.1589665361471178No Hit
CTTCCGTACTCTGCGTTGATACCACTGCTTCCGTACCTGTCTC30380.14891777268422568No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT30190.14798642387546984No Hit
GCGTTGATACCACTGCTTCCGTACTCTGCGTTGATACCACTGC27620.135388705778088No Hit
GGTATCAACGCAGAGTACGGAAGCAGTGGTATCAACGCAGAGT25210.1235752814143953No Hit
GCTTCCGTACTCTGCGTTGATACCACTGCTTCCGTACTCTGCG23920.11725191318652663No Hit
ACGCAGAGTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAG21490.10534045210612278No Hit
ATACCACTGCTTCCGTACTCTGCGTTGATACCACTGCTTCCGT21340.10460517673078923No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC12800.019.6562537
TATAACG609.2401623E-418.52
TCTTATA23450.016.56716537
ACGGACT4450.015.38202230
TCGTGTA750.004105855214.89
GTAAGAC1406.000919E-714.5357143
TTTAGAC1956.730261E-1014.230773
CGCGGTT1304.4477383E-614.23076918
TATACTG3000.014.1833335
GTTCTAG2500.014.0599991
TACGGAC4500.013.977777529
CTAACAC2551.8189894E-1213.7843133
ATAACGC950.001245652313.6315793
CGGACTG5200.013.51923131
ATAACAC1652.597717E-713.4545453
TAGTACT1652.597717E-713.4545454
CGTACCT11100.013.16666732
CTCTTAT36600.013.04098437
GAGTACT32450.012.94144811
GTATAGT1602.697714E-612.718751
Produced by FastQC (version 0.11.5)