##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727358.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2040052
Sequences flagged as poor quality	0
Sequence length	43
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.16876677653315	33.0	31.0	34.0	30.0	34.0
2	32.30802842280491	34.0	31.0	34.0	30.0	34.0
3	32.39946040591122	34.0	31.0	34.0	30.0	34.0
4	35.96189165766363	37.0	35.0	37.0	35.0	37.0
5	35.82736714554335	37.0	35.0	37.0	35.0	37.0
6	35.86654653900979	37.0	35.0	37.0	35.0	37.0
7	35.77342685382529	37.0	35.0	37.0	35.0	37.0
8	35.76681035581446	37.0	35.0	37.0	35.0	37.0
9	37.50684835484586	39.0	37.0	39.0	35.0	39.0
10	37.37732861711368	39.0	37.0	39.0	34.0	39.0
11	37.47980247562317	39.0	37.0	39.0	35.0	39.0
12	37.40210494634451	39.0	37.0	39.0	34.0	39.0
13	37.431769386270545	39.0	37.0	39.0	35.0	39.0
14	38.59329811200891	40.0	38.0	41.0	34.0	41.0
15	38.57080799901179	40.0	38.0	41.0	34.0	41.0
16	38.452501210753454	40.0	38.0	41.0	34.0	41.0
17	38.43895155613681	40.0	38.0	41.0	34.0	41.0
18	38.414821779052694	40.0	38.0	41.0	34.0	41.0
19	38.42591561391573	40.0	38.0	41.0	34.0	41.0
20	38.344176030807056	40.0	38.0	41.0	34.0	41.0
21	38.25853066490462	40.0	38.0	41.0	34.0	41.0
22	38.19662636050454	40.0	38.0	41.0	34.0	41.0
23	38.087226207959404	40.0	38.0	41.0	33.0	41.0
24	37.998473568320804	40.0	37.0	41.0	33.0	41.0
25	37.91513941801483	40.0	37.0	41.0	33.0	41.0
26	37.58258956144255	40.0	37.0	41.0	32.0	41.0
27	37.351196440090746	39.0	37.0	41.0	32.0	41.0
28	37.21461903912253	39.0	37.0	41.0	31.0	41.0
29	37.12770311737152	39.0	36.0	40.0	31.0	41.0
30	37.06679045436097	39.0	36.0	40.0	31.0	41.0
31	36.887467084172364	39.0	36.0	40.0	30.0	41.0
32	36.77549395799715	39.0	36.0	40.0	30.0	41.0
33	36.61794454259009	39.0	36.0	40.0	30.0	41.0
34	36.44774495944221	39.0	35.0	40.0	30.0	41.0
35	36.29121414552178	39.0	35.0	40.0	30.0	41.0
36	36.177040585239986	39.0	35.0	40.0	29.0	41.0
37	35.96650771646997	38.0	35.0	40.0	28.0	41.0
38	35.888374413985524	38.0	35.0	40.0	27.0	41.0
39	35.74879120728295	38.0	35.0	40.0	27.0	41.0
40	35.574311831267046	38.0	35.0	40.0	26.0	41.0
41	35.44940472105613	38.0	35.0	40.0	26.0	41.0
42	35.24042132259374	38.0	34.0	40.0	25.0	41.0
43	31.668077578414668	35.0	29.0	38.0	15.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	4.0
11	10.0
12	11.0
13	8.0
14	15.0
15	14.0
16	42.0
17	69.0
18	150.0
19	330.0
20	676.0
21	1411.0
22	2612.0
23	4285.0
24	6710.0
25	9758.0
26	13629.0
27	18052.0
28	23719.0
29	30643.0
30	39368.0
31	49546.0
32	62195.0
33	79309.0
34	103127.0
35	137413.0
36	195497.0
37	312781.0
38	556470.0
39	392196.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.87449535600073	18.310170525065047	13.123439990745334	25.691894128188892
2	17.56710123075294	21.50788313239074	35.08180183642378	25.84321380043254
3	19.132355449763043	22.770890153780392	30.566524774858678	27.53022962159788
4	15.038146086472306	15.104222833535616	32.783919233431305	37.07371184656078
5	15.157456770709764	36.44260048273279	33.414099248450526	14.98584349810691
6	37.112877514886875	34.46461168636878	13.466078315650778	14.956432483093568
7	29.338565879693263	31.02440526025807	20.426342073633418	19.21068678641525
8	28.049824220166936	35.41404826935784	19.15661953714905	17.37950797332617
9	26.68093754472925	14.50634591667271	17.829055337805112	40.98366120079292
10	15.386568577663706	27.527533611888323	33.72472858534978	23.361169225098184
11	36.71636801414866	21.309652891200813	20.31021758268907	21.66376151196146
12	22.048114459827495	24.90328677896446	29.65728324572119	23.39131551548686
13	32.866466148902084	19.02382880436381	23.977182934552648	24.132522112181455
14	23.274357712450467	19.769349016593697	25.827822035908888	31.128471235046952
15	27.20161054718213	26.785934868326887	22.054928011638918	23.957526572852064
16	25.565328726914803	24.896130098644544	24.994509943864177	24.544031230576476
17	24.531531549195805	27.026026787552475	25.534496179509148	22.90794548374257
18	24.57074623588026	24.407956267781408	27.14362182924749	23.877675667090838
19	24.57442261275693	27.035634385790164	25.07490005156731	23.31504294988559
20	24.348251907304324	25.516996625576212	25.82649854023329	24.30825292688618
21	23.864636783768255	26.87862858397727	24.696576361779012	24.56015827047546
22	24.57628531037444	25.909437602570918	23.989829671008387	25.524447416046257
23	24.822210414244342	24.77142739498797	24.252862181944383	26.153500008823304
24	24.083258662034105	25.41695015617249	24.648097205365353	25.851693976428052
25	25.287002488171872	24.97686333485617	24.368594526021887	25.36753965095007
26	24.99799024730742	24.14149247176052	25.57282853574321	25.287688745188845
27	25.502732283294737	25.881791248458374	25.224258989476738	23.391217478770148
28	25.898702582091044	24.900786842688323	24.937942758321846	24.262567816898787
29	24.09668969222353	25.474595745598645	26.311339122728246	24.11737543944958
30	24.38437843741238	25.64704233029354	25.84983127881054	24.11874795348354
31	24.4144757094427	24.130218249338743	25.298864930893917	26.15644111032464
32	24.1047777213522	25.036567695333257	25.35219690478478	25.506457678529763
33	25.63135645561976	24.95970690943172	24.42908318023266	24.97985345471586
34	24.950883604927718	25.08107636472012	25.31415865870086	24.653881371651313
35	24.229578461725485	25.563662102730717	26.008552723165877	24.19820671237792
36	24.56378562899377	24.63486224860935	25.44709644656117	25.354255675835713
37	23.142988512057535	23.867038683327678	26.56461698035148	26.425355824263303
38	23.776648830520006	23.901008405668094	26.870050371265048	25.452292392546855
39	23.849833239544875	23.18445804322635	27.05298688464804	25.912721832580736
40	23.95536976508442	23.222888436177115	28.657896955567796	24.163844843170665
41	24.09923864685802	22.71157794017015	27.8603192467643	25.328864166207527
42	23.108332532700146	22.943434775192003	29.03327954385476	24.914953148253083
43	23.265044224362907	21.947381733406797	28.932399762359	25.855174279871296
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	549.0
1	432.5
2	316.0
3	667.0
4	1018.0
5	1018.0
6	1227.0
7	1436.0
8	1457.0
9	1478.0
10	2090.5
11	2703.0
12	2703.0
13	5205.0
14	7707.0
15	11836.5
16	15966.0
17	12282.0
18	8598.0
19	8598.0
20	10127.0
21	11656.0
22	10655.0
23	9654.0
24	11885.5
25	14117.0
26	14117.0
27	17495.0
28	20873.0
29	24480.5
30	28088.0
31	33297.5
32	38507.0
33	38507.0
34	44619.0
35	50731.0
36	58819.5
37	66908.0
38	75933.0
39	84958.0
40	84958.0
41	94832.0
42	104706.0
43	115266.0
44	125826.0
45	137909.0
46	149992.0
47	149992.0
48	167318.0
49	184644.0
50	197740.5
51	210837.0
52	226720.0
53	242603.0
54	242603.0
55	207220.5
56	171838.0
57	153011.5
58	134185.0
59	121624.0
60	109063.0
61	109063.0
62	95465.5
63	81868.0
64	69355.5
65	56843.0
66	47271.5
67	37700.0
68	37700.0
69	30867.0
70	24034.0
71	19515.0
72	14996.0
73	12385.0
74	9774.0
75	9774.0
76	7984.5
77	6195.0
78	5083.0
79	3971.0
80	3209.5
81	2448.0
82	2448.0
83	1956.0
84	1464.0
85	1152.0
86	840.0
87	685.5
88	531.0
89	531.0
90	389.0
91	247.0
92	181.0
93	115.0
94	78.5
95	42.0
96	42.0
97	30.5
98	19.0
99	13.5
100	8.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	2040052.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	63.01524596272486
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.73186902936216	53.39399567766646
2	8.333197061709459	10.502369249989554
3	2.63249070451794	4.976611477193547
4	1.2761747021067273	3.216738509786502
5	0.7475535830309223	2.355363645250492
6	0.48967751917178576	1.8514289587816206
7	0.3300115830093819	1.4557032751719077
8	0.24436112289701437	1.23187810104664
9	0.18600166540190735	1.054884662529988
>10	0.9704016608532897	10.347172420713424
>50	0.03592278758440062	1.5294172446992045
>100	0.017436850505463834	2.1337675488125587
>500	0.002334157071189152	1.0128934299139207
>1k	0.002178546599776521	2.8090767304074618
>5k	2.3341570711891296E-4	1.0994789276257082
>10k+	1.5561047141260864E-4	1.0292201404110979
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGT	10714	0.5251826914215912	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	10278	0.503810687178562	No Hit
CTTCCGTACTCTGCGTTGATACCACTGCTTCCGTACTCTGCGT	8254	0.40459752986688574	No Hit
GAGTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTG	7152	0.35057929895904616	No Hit
TCCGTACTCTGCGTTGATACCACTGCTTCCGTACTCTGCGTTG	7019	0.3440598572977552	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	4020	0.19705380058939675	No Hit
TATCAACGCAGAGTACGGAAGCAGTGGTATCAACGCAGAGTAC	3680	0.18038755874850249	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3590	0.17597590649650108	No Hit
GTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCTGTCTC	3243	0.1589665361471178	No Hit
CTTCCGTACTCTGCGTTGATACCACTGCTTCCGTACCTGTCTC	3038	0.14891777268422568	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	3019	0.14798642387546984	No Hit
GCGTTGATACCACTGCTTCCGTACTCTGCGTTGATACCACTGC	2762	0.135388705778088	No Hit
GGTATCAACGCAGAGTACGGAAGCAGTGGTATCAACGCAGAGT	2521	0.1235752814143953	No Hit
GCTTCCGTACTCTGCGTTGATACCACTGCTTCCGTACTCTGCG	2392	0.11725191318652663	No Hit
ACGCAGAGTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAG	2149	0.10534045210612278	No Hit
ATACCACTGCTTCCGTACTCTGCGTTGATACCACTGCTTCCGT	2134	0.10460517673078923	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	4.901835835557133E-5	0.0	0.0	0.0
6	0.0	4.901835835557133E-5	0.0	0.0	1.47055075066714E-4
7	0.0	4.901835835557133E-5	0.0	0.0	1.9607343342228532E-4
8	0.0	4.901835835557133E-5	0.0	0.0	1.9607343342228532E-4
9	0.0	4.901835835557133E-5	0.0	4.901835835557133E-5	1.9607343342228532E-4
10	0.0	4.901835835557133E-5	0.0	4.901835835557133E-5	1.9607343342228532E-4
11	0.0	4.901835835557133E-5	0.0	4.901835835557133E-5	1.9607343342228532E-4
12	0.0	4.901835835557133E-5	0.0	4.901835835557133E-5	2.94110150133428E-4
13	0.0	4.901835835557133E-5	0.0	9.803671671114266E-5	2.94110150133428E-4
14	0.0	4.901835835557133E-5	0.0	9.803671671114266E-5	4.4116522520014194E-4
15	0.0	4.901835835557133E-5	0.0	2.450917917778566E-4	4.4116522520014194E-4
16	0.0	4.901835835557133E-5	0.0	4.4116522520014194E-4	4.901835835557132E-4
17	0.0	4.901835835557133E-5	0.0	7.842937336891413E-4	4.901835835557132E-4
18	0.0	4.901835835557133E-5	0.0	9.803671671114265E-4	4.901835835557132E-4
19	0.0	4.901835835557133E-5	0.0	0.0015195691090227111	5.392019419112846E-4
20	0.0	4.901835835557133E-5	0.0	0.002401899559422995	5.392019419112846E-4
21	4.901835835557133E-5	4.901835835557133E-5	0.0	0.0033332483681788503	5.392019419112846E-4
22	4.901835835557133E-5	4.901835835557133E-5	0.0	0.005441037777468418	5.392019419112846E-4
23	4.901835835557133E-5	4.901835835557133E-5	0.0	0.006960606886491129	6.862570169779986E-4
24	4.901835835557133E-5	4.901835835557133E-5	0.0	0.009509561520980838	6.862570169779986E-4
25	4.901835835557133E-5	4.901835835557133E-5	0.0	0.012744773172448546	7.842937336891413E-4
26	4.901835835557133E-5	4.901835835557133E-5	0.0	0.015097654373515968	8.333120920447126E-4
27	4.901835835557133E-5	4.901835835557133E-5	0.0	0.021666114393162527	8.333120920447126E-4
28	4.901835835557133E-5	4.901835835557133E-5	0.0	0.0500967622393939	8.333120920447126E-4
29	4.901835835557133E-5	4.901835835557133E-5	0.0	0.10563456225625621	8.333120920447126E-4
30	4.901835835557133E-5	4.901835835557133E-5	0.0	0.18533841294241518	8.333120920447126E-4
31	4.901835835557133E-5	4.901835835557133E-5	0.0	0.5473389893983095	9.313488087558553E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTATAC	1280	0.0	19.65625	37
TATAACG	60	9.2401623E-4	18.5	2
TCTTATA	2345	0.0	16.567165	37
ACGGACT	445	0.0	15.382022	30
TCGTGTA	75	0.0041058552	14.8	9
GTAAGAC	140	6.000919E-7	14.535714	3
TTTAGAC	195	6.730261E-10	14.23077	3
CGCGGTT	130	4.4477383E-6	14.230769	18
TATACTG	300	0.0	14.183333	5
GTTCTAG	250	0.0	14.059999	1
TACGGAC	450	0.0	13.9777775	29
CTAACAC	255	1.8189894E-12	13.784313	3
ATAACGC	95	0.0012456523	13.631579	3
CGGACTG	520	0.0	13.519231	31
ATAACAC	165	2.597717E-7	13.454545	3
TAGTACT	165	2.597717E-7	13.454545	4
CGTACCT	1110	0.0	13.166667	32
CTCTTAT	3660	0.0	13.040984	37
GAGTACT	3245	0.0	12.941448	11
GTATAGT	160	2.697714E-6	12.71875	1
>>END_MODULE