FastQCFastQC Report
Thu 9 Feb 2017
SRR2727357.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727357.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1725258
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT88040.5103004883907218No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT35650.20663576114413035No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28770.16675766754885357No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT28150.16316400213765128No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTAAGT609.239375E-418.52
TCTTATA20000.017.29749937
CTTATAC10750.016.86511637
GGTATCA20600.016.344661
TATAAGA1951.8189894E-1216.1282062
AACGCAG36450.015.8861475
CGAGTAT957.062953E-515.57894818
GTTATAG1101.452628E-515.1363641
ACCGTCC1405.9993545E-714.5357148
TATGCCG908.276977E-414.38888810
CTCGTTA1051.6563901E-414.09523822
CTCTTAT32050.013.96879837
ATTATAC2009.840733E-1013.8750013
TCAACGC41600.013.8753
ATCAACG42050.013.7705122
CAACGCA41950.013.7151374
TCTATAG1356.5710647E-613.7037033
CTAGAGT2457.2759576E-1213.5918364
TATCAAC42850.013.5565941
GTATTAG1652.5969348E-713.4545451
Produced by FastQC (version 0.11.5)