##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727357.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1725258
Sequences flagged as poor quality	0
Sequence length	43
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.16506168932415	33.0	31.0	34.0	30.0	34.0
2	32.31206636920391	34.0	31.0	34.0	30.0	34.0
3	32.39318699000381	34.0	31.0	34.0	30.0	34.0
4	35.96339388079928	37.0	35.0	37.0	35.0	37.0
5	35.850864624305466	37.0	35.0	37.0	35.0	37.0
6	35.862025853524514	37.0	35.0	37.0	35.0	37.0
7	35.81220373996237	37.0	35.0	37.0	35.0	37.0
8	35.78783173299298	37.0	35.0	37.0	35.0	37.0
9	37.515216274899174	39.0	37.0	39.0	35.0	39.0
10	37.355698683906986	39.0	37.0	39.0	34.0	39.0
11	37.46305943806665	39.0	37.0	39.0	35.0	39.0
12	37.36456866161467	39.0	37.0	39.0	34.0	39.0
13	37.4257096619752	39.0	37.0	39.0	35.0	39.0
14	38.6028408504699	40.0	38.0	41.0	34.0	41.0
15	38.57108907769157	40.0	38.0	41.0	34.0	41.0
16	38.51123715989145	40.0	38.0	41.0	34.0	41.0
17	38.473352391352485	40.0	38.0	41.0	34.0	41.0
18	38.42221511217453	40.0	38.0	41.0	34.0	41.0
19	38.42852895045263	40.0	38.0	41.0	34.0	41.0
20	38.31013970084474	40.0	38.0	41.0	34.0	41.0
21	38.22753930136826	40.0	38.0	41.0	34.0	41.0
22	38.16161582789357	40.0	38.0	41.0	34.0	41.0
23	38.05205018611709	40.0	38.0	41.0	33.0	41.0
24	37.947417139929215	40.0	37.0	41.0	33.0	41.0
25	37.86430783106063	40.0	37.0	41.0	33.0	41.0
26	37.51147364626044	40.0	37.0	41.0	32.0	41.0
27	37.29522425051789	39.0	37.0	41.0	31.0	41.0
28	37.16134688261118	39.0	36.0	41.0	31.0	41.0
29	37.11611074981249	39.0	36.0	40.0	31.0	41.0
30	37.03039197615661	39.0	36.0	40.0	31.0	41.0
31	36.81849671179615	39.0	36.0	40.0	30.0	41.0
32	36.71837429532279	39.0	36.0	40.0	30.0	41.0
33	36.53859538689286	39.0	36.0	40.0	30.0	41.0
34	36.4011162388466	39.0	35.0	40.0	30.0	41.0
35	36.24713173334075	39.0	35.0	40.0	29.0	41.0
36	36.10586474602639	39.0	35.0	40.0	29.0	41.0
37	35.88459233343651	38.0	35.0	40.0	27.0	41.0
38	35.781098247334604	38.0	35.0	40.0	27.0	41.0
39	35.62545543912852	38.0	35.0	40.0	26.0	41.0
40	35.48682168116305	38.0	35.0	40.0	26.0	41.0
41	35.359625632803905	38.0	34.0	40.0	25.0	41.0
42	35.16376043467122	38.0	34.0	40.0	24.0	41.0
43	31.745138408284443	35.0	29.0	38.0	15.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	6.0
11	10.0
12	5.0
13	6.0
14	15.0
15	9.0
16	38.0
17	62.0
18	136.0
19	331.0
20	680.0
21	1325.0
22	2273.0
23	3887.0
24	5970.0
25	8665.0
26	11992.0
27	15858.0
28	21016.0
29	26832.0
30	33994.0
31	42703.0
32	53244.0
33	67743.0
34	86954.0
35	116072.0
36	162870.0
37	258252.0
38	464825.0
39	339483.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.21107799529114	18.885349321666673	12.705519985996297	26.198052697045892
2	18.510101097922746	21.178687477467136	34.72408184746861	25.587129577141503
3	18.29001807265928	22.126719597880435	31.006087205507814	28.577175123952475
4	14.574168037476134	15.720373416613631	33.952139332204226	35.75331921370601
5	14.255606987476654	37.24590756860713	34.38905949139201	14.109425952524202
6	36.12056863379274	35.82542437131142	14.07650334036996	13.977503654525874
7	30.14198456114969	31.247384449166443	20.104703180625737	18.50592780905812
8	28.03203926601123	35.31958698351203	18.64747185638322	18.00090189409352
9	27.094672217140857	13.595647723412963	18.206204521294787	41.10347553815139
10	15.426678212765859	27.908753357468854	33.88223674372181	22.782331686043477
11	37.37933688758435	21.038128790012856	21.232650420980516	20.34988390142228
12	21.653804822235283	25.209273047857188	30.131087640225402	23.005834489682123
13	31.824631446427144	19.725165743326507	23.65118724271964	24.799015567526713
14	22.38476795934289	20.01752781323141	25.845409787985336	31.75229443944036
15	25.446686814377905	28.23455970063608	21.615433749618898	24.703319735367117
16	24.94009591608907	26.360347264003416	23.790470758576397	24.909086061331116
17	24.397336514306843	26.429322454960356	25.14209468960584	24.03124634112695
18	24.455878483102236	25.580927606189913	25.64990279714686	24.313291113560986
19	25.353599287758698	25.530268516361033	25.734122084928746	23.38201011095152
20	25.059788159220243	25.325893286685236	25.49728794186145	24.11703061223307
21	24.73780733084559	25.519197708400714	25.62132736089327	24.121667599860427
22	25.31592376328642	25.20185386765342	25.03874782786111	24.443474541199055
23	24.670107311486166	25.274074949949515	25.213678186103177	24.84213955246114
24	24.620665430909465	25.202781265178892	25.286247042471327	24.89030626144032
25	24.92566329209892	24.87546790103277	25.33273284343559	24.866135963432715
26	24.46834038735076	25.18933400105955	25.62022607633177	24.722099535257914
27	24.735546799377254	25.533746257081553	25.25199129637422	24.478715647166975
28	24.622520225960407	25.48366679070608	25.331747483564776	24.56206549976873
29	24.56849932010169	25.76408861747055	25.388724469035935	24.27868759339183
30	24.804579952679543	25.646135244699636	25.132299053243052	24.416985749377773
31	24.684250123749607	24.987451152233465	25.473755229652607	24.85454349436432
32	24.183571384685653	25.420777646010045	25.508764486239162	24.88688648306514
33	24.396003380363982	24.960440699304105	25.599881293116738	25.043674627215175
34	24.636836925259875	24.749052025841934	26.069955913840133	24.54415513505806
35	24.420579414788975	24.808927128580187	26.054016268871088	24.716477187759743
36	24.27138433787874	24.45895048740536	26.333858472182133	24.935806702533768
37	23.969748292719117	24.109727356719983	26.608252215031026	25.312272135529874
38	23.591949725780147	24.041505676252477	27.045809960017575	25.320734637949805
39	23.398007718265905	23.867966414298618	27.596857977183703	25.137167890251778
40	23.547840380974904	23.716336918884014	27.917157897543436	24.818664802597638
41	23.127091716137528	23.568127201844593	28.28255252257923	25.02222855943865
42	22.62502188078537	23.852084731674914	28.335240294495083	25.18765309304463
43	22.728078930803395	22.920745766720106	28.252064328929354	26.099110973547145
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	223.0
1	233.5
2	244.0
3	575.0
4	906.0
5	906.0
6	1035.5
7	1165.0
8	1233.0
9	1301.0
10	1902.0
11	2503.0
12	2503.0
13	4422.0
14	6341.0
15	10011.5
16	13682.0
17	10794.0
18	7906.0
19	7906.0
20	9383.0
21	10860.0
22	10214.5
23	9569.0
24	11815.5
25	14062.0
26	14062.0
27	17160.5
28	20259.0
29	23791.0
30	27323.0
31	32025.5
32	36728.0
33	36728.0
34	42453.0
35	48178.0
36	55126.0
37	62074.0
38	70233.5
39	78393.0
40	78393.0
41	87764.5
42	97136.0
43	106360.5
44	115585.0
45	124061.5
46	132538.0
47	132538.0
48	141103.5
49	149669.0
50	153081.0
51	156493.0
52	155396.5
53	154300.0
54	154300.0
55	147518.5
56	140737.0
57	131190.5
58	121644.0
59	110478.5
60	99313.0
61	99313.0
62	86797.5
63	74282.0
64	62634.5
65	50987.0
66	42333.5
67	33680.0
68	33680.0
69	27326.5
70	20973.0
71	17158.0
72	13343.0
73	10924.5
74	8506.0
75	8506.0
76	6919.5
77	5333.0
78	4521.0
79	3709.0
80	2965.5
81	2222.0
82	2222.0
83	1789.0
84	1356.0
85	1068.0
86	780.0
87	638.5
88	497.0
89	497.0
90	390.0
91	283.0
92	196.0
93	109.0
94	79.0
95	49.0
96	49.0
97	29.0
98	9.0
99	8.5
100	8.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1725258.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	70.98553159856415
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.63051325870778	61.49513036325831
2	7.407514317769517	10.51652683341689
3	2.2859451087359144	4.868070862462693
4	1.1103564425521444	3.152769693538181
5	0.66393514135193	2.356489447791729
6	0.4329065510887635	1.8438060996922108
7	0.27866902760137724	1.3847028345037093
8	0.20997006893549935	1.1923869570538865
9	0.16399155192129505	1.0476924741725997
>10	0.7876620865325588	9.410808568768463
>50	0.023463408422319462	1.1202455229926582
>100	0.004664025581729867	0.5233558844894954
>500	8.180215984224063E-5	0.039253763863630255
>1k	2.4540647952672187E-4	0.5375326806000373
>5k	8.180215984224063E-5	0.5112280133955632
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	8804	0.5103004883907218	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	3565	0.20663576114413035	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2877	0.16675766754885357	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	2815	0.16316400213765128	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	5.796234534197204E-5
8	0.0	0.0	0.0	0.0	5.796234534197204E-5
9	0.0	0.0	0.0	5.796234534197204E-5	1.7388703602591612E-4
10	5.796234534197204E-5	0.0	0.0	1.1592469068394408E-4	1.7388703602591612E-4
11	5.796234534197204E-5	0.0	0.0	1.1592469068394408E-4	1.7388703602591612E-4
12	5.796234534197204E-5	0.0	0.0	2.3184938136788815E-4	1.7388703602591612E-4
13	5.796234534197204E-5	0.0	0.0	2.3184938136788815E-4	2.3184938136788815E-4
14	5.796234534197204E-5	0.0	0.0	2.3184938136788815E-4	2.3184938136788815E-4
15	5.796234534197204E-5	0.0	0.0	2.898117267098602E-4	2.3184938136788815E-4
16	5.796234534197204E-5	0.0	0.0	5.216611080777483E-4	3.4777407205183224E-4
17	5.796234534197204E-5	0.0	0.0	8.114728347876085E-4	4.0573641739380427E-4
18	5.796234534197204E-5	0.0	0.0	9.853598708135247E-4	4.0573641739380427E-4
19	1.1592469068394408E-4	0.0	0.0	0.0011012845614974689	4.0573641739380427E-4
20	1.1592469068394408E-4	0.0	0.0	0.001391096288207329	4.0573641739380427E-4
21	1.1592469068394408E-4	0.0	0.0	0.0023184938136788817	4.0573641739380427E-4
22	1.1592469068394408E-4	0.0	0.0	0.003941439483254099	4.0573641739380427E-4
23	1.1592469068394408E-4	0.0	0.0	0.004926799354067623	5.216611080777483E-4
24	1.1592469068394408E-4	0.0	0.0	0.005854196879539176	5.216611080777483E-4
25	1.1592469068394408E-4	0.0	0.0	0.00654974502364284	5.216611080777483E-4
26	1.1592469068394408E-4	0.0	0.0	0.007187330822404533	5.216611080777483E-4
27	1.1592469068394408E-4	0.0	0.0	0.011766356104420325	5.796234534197204E-4
28	1.1592469068394408E-4	0.0	0.0	0.040573641739380426	5.796234534197204E-4
29	1.1592469068394408E-4	0.0	0.0	0.10091244324037332	5.796234534197204E-4
30	1.1592469068394408E-4	0.0	0.0	0.18124825388434657	5.796234534197204E-4
31	1.1592469068394408E-4	0.0	0.0	0.5615971640183671	5.796234534197204E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTAAGT	60	9.239375E-4	18.5	2
TCTTATA	2000	0.0	17.297499	37
CTTATAC	1075	0.0	16.865116	37
GGTATCA	2060	0.0	16.34466	1
TATAAGA	195	1.8189894E-12	16.128206	2
AACGCAG	3645	0.0	15.886147	5
CGAGTAT	95	7.062953E-5	15.578948	18
GTTATAG	110	1.452628E-5	15.136364	1
ACCGTCC	140	5.9993545E-7	14.535714	8
TATGCCG	90	8.276977E-4	14.388888	10
CTCGTTA	105	1.6563901E-4	14.095238	22
CTCTTAT	3205	0.0	13.968798	37
ATTATAC	200	9.840733E-10	13.875001	3
TCAACGC	4160	0.0	13.875	3
ATCAACG	4205	0.0	13.770512	2
CAACGCA	4195	0.0	13.715137	4
TCTATAG	135	6.5710647E-6	13.703703	3
CTAGAGT	245	7.2759576E-12	13.591836	4
TATCAAC	4285	0.0	13.556594	1
GTATTAG	165	2.5969348E-7	13.454545	1
>>END_MODULE