FastQCFastQC Report
Thu 9 Feb 2017
SRR2727356.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727356.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1017342
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT55370.5442614184807076No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT22090.21713445429363973No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18540.18223960084219465No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT14710.14459247725936802No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTTAG358.868692E-426.4285729
TCGTAAT500.007034341318.4999982
ACTATAC852.722617E-517.4117643
GTATAGT650.001579765717.0769211
TATAGAG1005.8764563E-616.652
GTACTAG803.3820877E-416.18751
AACGCAG24650.015.8357015
TTAGAAC1201.93487E-615.4166673
CTAACAC1201.93487E-615.4166673
TAGACAG1954.1836756E-1115.1794875
CTTATAC5400.015.07407537
GAGTACT17050.014.86510311
GGTATCA14850.014.8249161
TATACTG1508.096504E-814.85
CTCTTAT15200.014.72697437
TCTTATA9200.014.67934837
AGTACTT17700.014.52824812
GTGTTAC908.2728633E-414.3888881
CTACTCG908.2728633E-414.38888814
GTATAAG1803.3269316E-914.3888881
Produced by FastQC (version 0.11.5)