##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727356.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1017342
Sequences flagged as poor quality	0
Sequence length	43
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.17076460030157	33.0	31.0	34.0	30.0	34.0
2	32.316426531097704	34.0	31.0	34.0	30.0	34.0
3	32.391930147384066	34.0	31.0	34.0	30.0	34.0
4	35.96313727340462	37.0	35.0	37.0	35.0	37.0
5	35.85173619097609	37.0	35.0	37.0	35.0	37.0
6	35.867075182190455	37.0	35.0	37.0	35.0	37.0
7	35.81127388823031	37.0	35.0	37.0	35.0	37.0
8	35.78575936115878	37.0	35.0	37.0	35.0	37.0
9	37.52533169769851	39.0	37.0	39.0	35.0	39.0
10	37.36028100678041	39.0	37.0	39.0	34.0	39.0
11	37.46905858600156	39.0	37.0	39.0	35.0	39.0
12	37.37245390439007	39.0	37.0	39.0	34.0	39.0
13	37.4343023290103	39.0	37.0	39.0	35.0	39.0
14	38.60944107291353	40.0	38.0	41.0	34.0	41.0
15	38.57233555677442	40.0	38.0	41.0	34.0	41.0
16	38.51383703808552	40.0	38.0	41.0	34.0	41.0
17	38.46706122424907	40.0	38.0	41.0	34.0	41.0
18	38.41926018978869	40.0	38.0	41.0	34.0	41.0
19	38.437250206911735	40.0	38.0	41.0	34.0	41.0
20	38.31413428325971	40.0	38.0	41.0	34.0	41.0
21	38.24503559275052	40.0	38.0	41.0	34.0	41.0
22	38.16779509742053	40.0	38.0	41.0	34.0	41.0
23	38.05520758997466	40.0	38.0	41.0	33.0	41.0
24	37.937508723713364	40.0	37.0	41.0	33.0	41.0
25	37.86078821084748	40.0	37.0	41.0	33.0	41.0
26	37.50675780612616	40.0	37.0	41.0	32.0	41.0
27	37.28699001908896	39.0	37.0	41.0	31.0	41.0
28	37.13912037446601	39.0	36.0	41.0	31.0	41.0
29	37.09583109711385	39.0	36.0	40.0	31.0	41.0
30	37.014495617009814	39.0	36.0	40.0	31.0	41.0
31	36.815768935127025	39.0	36.0	40.0	30.0	41.0
32	36.71073346033094	39.0	36.0	40.0	30.0	41.0
33	36.517863216106285	39.0	36.0	40.0	30.0	41.0
34	36.38282111620281	39.0	35.0	40.0	30.0	41.0
35	36.23359892740101	39.0	35.0	40.0	29.0	41.0
36	36.085875742867195	39.0	35.0	40.0	29.0	41.0
37	35.859108343113725	38.0	35.0	40.0	27.0	41.0
38	35.7421309648083	38.0	35.0	40.0	27.0	41.0
39	35.57508979281304	38.0	35.0	40.0	26.0	41.0
40	35.43410180647216	38.0	34.0	40.0	26.0	41.0
41	35.290876617695915	38.0	34.0	40.0	25.0	41.0
42	35.088289876953866	38.0	34.0	40.0	24.0	41.0
43	31.68867794704239	35.0	29.0	38.0	15.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	3.0
11	3.0
12	0.0
13	5.0
14	8.0
15	20.0
16	21.0
17	47.0
18	103.0
19	212.0
20	403.0
21	822.0
22	1355.0
23	2362.0
24	3546.0
25	5133.0
26	7012.0
27	9450.0
28	12346.0
29	15777.0
30	19978.0
31	25095.0
32	31604.0
33	40135.0
34	51826.0
35	69031.0
36	96703.0
37	152005.0
38	273355.0
39	198982.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.245478904832396	18.918121929498636	12.67892213238026	26.157477033288707
2	18.81599304855201	21.29274128070993	34.20275580876441	25.688509861973653
3	18.79387659213912	22.021306502631365	30.763204507432114	28.421612397797396
4	14.743911093811127	15.840395855081182	33.30463108767749	36.11106196343019
5	14.560491948626911	36.95777821027737	34.19410581692292	14.287624024172796
6	36.39837930607406	35.250092889116935	14.07854978954963	14.27297801525937
7	30.00230011146694	30.79190675308795	20.16843893204055	19.037354203404558
8	28.345138606289723	34.66071389955393	18.599743252514887	18.394404241641453
9	26.787943484098758	13.588744001525544	18.18051353428837	41.442798980087325
10	15.6722124909814	27.98734348921012	33.68916254317624	22.651281476632242
11	37.51177086957975	20.93189900741344	20.96069954843111	20.595630574575708
12	21.60610689424009	25.360006762720893	29.940079147425347	23.093807195613667
13	31.976759044647718	19.672440536220858	23.514609639629544	24.83619077950188
14	22.486243564111184	20.238228638943443	25.771667738086112	31.503860058859264
15	25.61626277102489	28.245270518665304	21.497490519412352	24.640976190897458
16	25.10758427352847	26.065177688525587	23.751206575566524	25.076031462379415
17	24.415879812295177	26.33873368051255	25.159385929215546	24.086000577976726
18	24.45460818485819	25.442968048109684	25.579795191784083	24.52262857524805
19	25.52042479323571	25.20519156783068	25.69932235177551	23.575061287158103
20	25.019413333962426	25.395196502257843	25.44080555014931	24.14458461363042
21	24.817219774667713	25.311448853974376	25.522390700472407	24.348940670885504
22	25.20951656375142	25.126162096915294	25.175997845365668	24.488323493967616
23	24.874034493808374	25.029734346955106	25.308499993119327	24.787731166117197
24	24.76020846480338	25.11967460303418	25.254142658024538	24.8659742741379
25	24.927113989199306	24.79824876983355	25.38910218982407	24.885535051143076
26	24.609030198301063	25.076424643826755	25.588052002178223	24.726493155693955
27	24.804637968352825	25.330321563446706	25.342608483676088	24.522431984524378
28	24.845332248152538	25.32963349591386	25.412496485940817	24.412537769992785
29	24.61826996231356	25.61272413799883	25.295525005357096	24.47348089433052
30	24.681178993887993	25.552862262641273	25.275276160819075	24.490682582651655
31	24.570006939652544	24.764435165362286	25.60987357250561	25.05568432247956
32	24.31483218032874	25.32717611186798	25.479239036626815	24.878752671176457
33	24.54386037340442	24.94018727232337	25.45889189672696	25.057060457545248
34	24.557425133337656	24.81210841585229	25.831234727358154	24.799231723451896
35	24.604410316294814	24.73612610115379	25.925008502548796	24.734455080002597
36	24.289177090889787	24.427970141800888	26.22461276542205	25.05824000188727
37	24.13003690007883	24.16453857208294	26.427985869058784	25.277438658779445
38	23.78128495628805	24.079119902648273	26.953767759514502	25.185827381549174
39	23.567984021105982	23.84576671365185	27.368279300372933	25.217969964869237
40	23.700682759583305	23.68574186458438	27.628270532426658	24.985304843405657
41	23.25913999422023	23.62008056287856	28.051530360488407	25.0692490824128
42	22.919332928356443	23.87161839381447	27.858085088397015	25.35096358943207
43	23.103145254987997	23.098230486896245	27.82604080043879	25.97258345767697
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	115.0
1	132.5
2	150.0
3	300.0
4	450.0
5	450.0
6	543.5
7	637.0
8	673.5
9	710.0
10	1088.5
11	1467.0
12	1467.0
13	2689.0
14	3911.0
15	6322.0
16	8733.0
17	6813.0
18	4893.0
19	4893.0
20	5852.5
21	6812.0
22	6483.0
23	6154.0
24	7371.0
25	8588.0
26	8588.0
27	10435.0
28	12282.0
29	14287.0
30	16292.0
31	19033.5
32	21775.0
33	21775.0
34	25221.0
35	28667.0
36	32581.5
37	36496.0
38	40930.5
39	45365.0
40	45365.0
41	50346.5
42	55328.0
43	60234.5
44	65141.0
45	70486.5
46	75832.0
47	75832.0
48	80225.5
49	84619.0
50	87194.0
51	89769.0
52	89244.5
53	88720.0
54	88720.0
55	85472.0
56	82224.0
57	77528.0
58	72832.0
59	66689.0
60	60546.0
61	60546.0
62	53550.0
63	46554.0
64	39726.5
65	32899.0
66	27324.5
67	21750.0
68	21750.0
69	17574.0
70	13398.0
71	10987.5
72	8577.0
73	7092.5
74	5608.0
75	5608.0
76	4613.0
77	3618.0
78	3060.0
79	2502.0
80	2033.0
81	1564.0
82	1564.0
83	1258.0
84	952.0
85	784.0
86	616.0
87	510.5
88	405.0
89	405.0
90	319.5
91	234.0
92	165.5
93	97.0
94	71.0
95	45.0
96	45.0
97	29.0
98	13.0
99	7.5
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1017342.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	78.53201839431884
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.15080137198775	70.01192373213208
2	6.741747197894825	10.588860299098478
3	1.8676610206854998	4.40013568892478
4	0.7765974284019322	2.4395105412896503
5	0.42603989491783467	1.6728886432200527
6	0.2571315335157088	1.2115834991889032
7	0.17231637771117256	0.9472647060839338
8	0.12155341281279912	0.7636667880725577
9	0.08261729307972206	0.5839292501843008
>10	0.38496197498733276	5.0220129051699685
>50	0.013682075296788502	0.7317069603477
>100	0.00438883848024591	0.5362972798294214
>500	0.0	0.0
>1k	3.7618517128504335E-4	0.5449621403251449
>5k	1.2539505709501447E-4	0.5452575661330551
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	5537	0.5442614184807076	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	2209	0.21713445429363973	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1854	0.18223960084219465	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	1471	0.14459247725936802	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	9.829536183505644E-5	0.0
6	0.0	0.0	0.0	9.829536183505644E-5	0.0
7	0.0	0.0	0.0	9.829536183505644E-5	0.0
8	0.0	0.0	0.0	9.829536183505644E-5	0.0
9	0.0	0.0	0.0	9.829536183505644E-5	0.0
10	9.829536183505644E-5	0.0	0.0	9.829536183505644E-5	0.0
11	9.829536183505644E-5	0.0	0.0	1.965907236701129E-4	0.0
12	9.829536183505644E-5	0.0	0.0	1.965907236701129E-4	9.829536183505644E-5
13	9.829536183505644E-5	0.0	0.0	1.965907236701129E-4	1.965907236701129E-4
14	9.829536183505644E-5	0.0	0.0	4.914768091752822E-4	1.965907236701129E-4
15	9.829536183505644E-5	0.0	0.0	4.914768091752822E-4	2.9488608550516936E-4
16	9.829536183505644E-5	0.0	0.0	5.897721710103387E-4	2.9488608550516936E-4
17	9.829536183505644E-5	0.0	0.0	6.880675328453952E-4	2.9488608550516936E-4
18	9.829536183505644E-5	0.0	0.0	6.880675328453952E-4	3.931814473402258E-4
19	9.829536183505644E-5	0.0	0.0	8.84658256515508E-4	3.931814473402258E-4
20	9.829536183505644E-5	0.0	0.0	0.001081248980185621	3.931814473402258E-4
21	9.829536183505644E-5	0.0	0.0	0.0015727257893609031	3.931814473402258E-4
22	9.829536183505644E-5	0.0	0.0	0.0027522701313815808	3.931814473402258E-4
23	9.829536183505644E-5	0.0	0.0	0.0031454515787218062	3.931814473402258E-4
24	1.965907236701129E-4	0.0	0.0	0.003931814473402258	3.931814473402258E-4
25	1.965907236701129E-4	0.0	0.0	0.00471817736808271	3.931814473402258E-4
26	1.965907236701129E-4	0.0	0.0	0.005799426348268331	3.931814473402258E-4
27	1.965907236701129E-4	0.0	0.0	0.010222717630845871	3.931814473402258E-4
28	1.965907236701129E-4	0.0	0.0	0.034698262727774924	3.931814473402258E-4
29	1.965907236701129E-4	0.0	0.0	0.09043173288825193	3.931814473402258E-4
30	1.965907236701129E-4	0.0	0.0	0.16307200528435864	3.931814473402258E-4
31	1.965907236701129E-4	0.0	0.0	0.45540241138181653	3.931814473402258E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCTTAG	35	8.868692E-4	26.42857	29
TCGTAAT	50	0.0070343413	18.499998	2
ACTATAC	85	2.722617E-5	17.411764	3
GTATAGT	65	0.0015797657	17.076921	1
TATAGAG	100	5.8764563E-6	16.65	2
GTACTAG	80	3.3820877E-4	16.1875	1
AACGCAG	2465	0.0	15.835701	5
TTAGAAC	120	1.93487E-6	15.416667	3
CTAACAC	120	1.93487E-6	15.416667	3
TAGACAG	195	4.1836756E-11	15.179487	5
CTTATAC	540	0.0	15.074075	37
GAGTACT	1705	0.0	14.865103	11
GGTATCA	1485	0.0	14.824916	1
TATACTG	150	8.096504E-8	14.8	5
CTCTTAT	1520	0.0	14.726974	37
TCTTATA	920	0.0	14.679348	37
AGTACTT	1770	0.0	14.528248	12
GTGTTAC	90	8.2728633E-4	14.388888	1
CTACTCG	90	8.2728633E-4	14.388888	14
GTATAAG	180	3.3269316E-9	14.388888	1
>>END_MODULE