FastQCFastQC Report
Thu 9 Feb 2017
SRR2727355.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727355.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences990441
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT40120.4050720840514478No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT15750.1590200728766277No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12760.12883150031147741No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT12170.12287455789895613No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC5650.020.95575337
GTCTAAA1002.8739305E-718.51
TTATACT1103.850073E-818.54
GCTTAAC1306.9667294E-1018.51
TCGCGGT500.007034211418.517
GGCGTCA1451.546141E-1017.86206812
GGGCGTC1351.1514203E-917.81481611
TCTTATA10550.017.18483537
GCGTCAG1401.8681021E-917.17857213
AGGGCGT1655.4569682E-1116.81818210
GGCTTAA803.3819748E-416.18751
TCTACTA700.00259215415.8571422
ATTGGAC700.00259215415.8571423
CGAGACG700.00259215415.85714223
TTTATAC1557.203198E-915.5161293
GGTATCA11000.015.4727281
GTGCAAC855.36322E-415.23529411
TACTTAC1101.4513858E-515.13636431
GTATATA1252.9575585E-614.8000011
ATTAGGT750.00410387214.83
Produced by FastQC (version 0.11.5)