##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727355.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	990441
Sequences flagged as poor quality	0
Sequence length	43
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.2008660788477	33.0	31.0	34.0	30.0	34.0
2	32.346531494556466	34.0	31.0	34.0	30.0	34.0
3	32.42621115240585	34.0	31.0	34.0	30.0	34.0
4	35.99419955353221	37.0	35.0	37.0	35.0	37.0
5	35.87897310390018	37.0	35.0	37.0	35.0	37.0
6	35.886361731794224	37.0	35.0	37.0	35.0	37.0
7	35.83521481844956	37.0	35.0	37.0	35.0	37.0
8	35.80549775302113	37.0	35.0	37.0	35.0	37.0
9	37.54523086180802	39.0	37.0	39.0	35.0	39.0
10	37.3816623100215	39.0	37.0	39.0	34.0	39.0
11	37.48818253686994	39.0	37.0	39.0	35.0	39.0
12	37.39610840019749	39.0	37.0	39.0	34.0	39.0
13	37.44785706569094	39.0	37.0	39.0	35.0	39.0
14	38.637551353387025	40.0	38.0	41.0	35.0	41.0
15	38.59937744903533	40.0	38.0	41.0	34.0	41.0
16	38.53669224113299	40.0	38.0	41.0	34.0	41.0
17	38.49564486930569	40.0	38.0	41.0	34.0	41.0
18	38.44177694582514	40.0	38.0	41.0	34.0	41.0
19	38.43920940268022	40.0	38.0	41.0	34.0	41.0
20	38.32300561063203	40.0	38.0	41.0	34.0	41.0
21	38.24949694126152	40.0	38.0	41.0	34.0	41.0
22	38.170790587223266	40.0	38.0	41.0	34.0	41.0
23	38.05806807270701	40.0	38.0	41.0	33.0	41.0
24	37.94993543280216	40.0	37.0	41.0	33.0	41.0
25	37.86530343554033	40.0	37.0	41.0	33.0	41.0
26	37.51060689127368	40.0	37.0	41.0	32.0	41.0
27	37.29816617042307	39.0	37.0	41.0	31.0	41.0
28	37.14915880905576	39.0	36.0	41.0	31.0	41.0
29	37.11340503876556	39.0	36.0	41.0	31.0	41.0
30	37.024647606470246	39.0	36.0	40.0	31.0	41.0
31	36.81517929891836	39.0	36.0	40.0	30.0	41.0
32	36.713587179852205	39.0	36.0	40.0	30.0	41.0
33	36.534197392878525	39.0	36.0	40.0	30.0	41.0
34	36.388254323074264	39.0	35.0	40.0	30.0	41.0
35	36.238232262194316	39.0	35.0	40.0	29.0	41.0
36	36.089529815506424	39.0	35.0	40.0	28.0	41.0
37	35.861449596694804	38.0	35.0	40.0	27.0	41.0
38	35.75812693537525	38.0	35.0	40.0	27.0	41.0
39	35.588329844988245	38.0	35.0	40.0	26.0	41.0
40	35.45550113535284	38.0	35.0	40.0	26.0	41.0
41	35.31900133374931	38.0	34.0	40.0	25.0	41.0
42	35.130318716612095	38.0	34.0	40.0	24.0	41.0
43	31.685970189037004	35.0	29.0	38.0	15.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	0.0
9	0.0
10	2.0
11	3.0
12	2.0
13	2.0
14	2.0
15	9.0
16	28.0
17	48.0
18	103.0
19	202.0
20	402.0
21	744.0
22	1383.0
23	2232.0
24	3490.0
25	4949.0
26	6939.0
27	9341.0
28	11963.0
29	15334.0
30	19492.0
31	24223.0
32	30673.0
33	38558.0
34	49914.0
35	66338.0
36	93263.0
37	146002.0
38	266767.0
39	198032.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.22957248336852	18.814851162260045	12.58399036388841	26.371585990483027
2	18.736906085269087	21.116250236005982	34.57459858790175	25.57224509082318
3	18.37151329559257	21.947193219989884	31.02658310792869	28.654710376488858
4	14.739696761341664	15.629603378696963	33.7993883532689	35.83131150669247
5	14.374404936790782	37.09186110025736	34.267563640842816	14.26617032210904
6	36.229618927326314	35.58929810054309	14.093418992145923	14.087663979984674
7	30.28994155128877	31.08019558964138	20.052481672305568	18.57738118676428
8	28.190068868312196	34.98764691687844	18.57223196535685	18.250052249452516
9	27.17849927456557	13.513374345367366	18.028231868430325	41.27989451163673
10	15.538028009745153	27.709979695913233	33.89358881548724	22.85840347885437
11	37.493904230539734	20.947941371570845	21.093533082737892	20.464621315151533
12	21.763941516960628	25.090237581037133	29.832064706529714	23.31375619547252
13	32.03623436428823	19.579359093575487	23.33051640632809	25.05389013580819
14	22.471505117417394	20.09337254818813	25.484102536142988	31.951019798251483
15	25.648776656055233	28.110003523682884	21.38128369080036	24.859936129461524
16	25.044601344249685	26.18762753157432	23.65693665750913	25.11083446666687
17	24.68587225286514	26.18187251941307	24.824901230865848	24.307353996855944
18	24.724339965732437	25.41948485573598	25.306302949898075	24.549872228633507
19	25.607582884795764	25.40050341211642	25.406359389403306	23.585554313684508
20	25.318822625476933	25.112853769179587	25.157581319836314	24.410742285507165
21	24.939294718211382	25.27056129542295	25.42079740236925	24.36934658399642
22	25.52751754016645	25.0196629582176	24.728580501009144	24.7242390006068
23	24.95383369630296	24.99856124695969	24.96231476685638	25.085290289880973
24	24.852969535792642	24.99603711881879	25.020268748971418	25.130724596417153
25	25.135570922447677	24.65810684331525	25.02047067922269	25.18585155501438
26	24.69405042804165	24.961002220223115	25.30529329864172	25.039654053093518
27	25.13769119008603	25.340429162363026	24.84903189589284	24.6728477516581
28	24.857714896697534	25.32861624266362	24.989979211280634	24.823689649358215
29	24.930712682532327	25.5088389919238	25.05146697279293	24.508981352750947
30	25.116488513702485	25.413628878449096	24.73897990894965	24.730902698898774
31	24.961608010976928	24.751398619402874	25.169697134912628	25.11729623470757
32	24.455368871038257	25.20039053310596	25.118608781340836	25.225631814514948
33	24.624485456478478	24.779567889455304	25.241483339239796	25.354463314826425
34	24.861147710969153	24.628826956880822	25.683508659274	24.82651667287602
35	24.69576683517746	24.647606470249112	25.645242876657974	25.011383817915455
36	24.540886332451908	24.266362155847748	25.910882122206168	25.281869389494176
37	24.228904094236807	23.98335690869017	26.23498017549758	25.552758821575438
38	23.975582594016203	23.782335343548986	26.721934976439787	25.520147085995028
39	23.833019836618234	23.61533902574712	27.241602478088044	25.310038659546606
40	23.86229972305266	23.453592894478316	27.5057272467517	25.178380135717322
41	23.441880939904546	23.373729480100277	27.969762964174542	25.214626615820627
42	22.951796220067628	23.5414325537816	27.949468973921714	25.557302252229057
43	23.038121402486368	22.635876341952727	27.87324030406657	26.452761951494335
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	77.0
1	91.0
2	105.0
3	224.5
4	344.0
5	344.0
6	413.0
7	482.0
8	494.5
9	507.0
10	787.0
11	1067.0
12	1067.0
13	1947.5
14	2828.0
15	4560.5
16	6293.0
17	5032.5
18	3772.0
19	3772.0
20	4551.0
21	5330.0
22	5188.5
23	5047.0
24	6227.5
25	7408.0
26	7408.0
27	9256.5
28	11105.0
29	12959.0
30	14813.0
31	17542.5
32	20272.0
33	20272.0
34	23486.0
35	26700.0
36	30823.0
37	34946.0
38	39696.0
39	44446.0
40	44446.0
41	49534.5
42	54623.0
43	60086.0
44	65549.0
45	70620.0
46	75691.0
47	75691.0
48	80780.5
49	85870.0
50	88290.5
51	90711.0
52	90412.0
53	90113.0
54	90113.0
55	86093.0
56	82073.0
57	76497.5
58	70922.0
59	64921.5
60	58921.0
61	58921.0
62	51754.0
63	44587.0
64	37675.0
65	30763.0
66	25458.5
67	20154.0
68	20154.0
69	16437.0
70	12720.0
71	10424.5
72	8129.0
73	6599.5
74	5070.0
75	5070.0
76	4241.0
77	3412.0
78	2827.0
79	2242.0
80	1818.0
81	1394.0
82	1394.0
83	1096.0
84	798.0
85	648.5
86	499.0
87	422.0
88	345.0
89	345.0
90	266.5
91	188.0
92	133.0
93	78.0
94	56.0
95	34.0
96	34.0
97	22.0
98	10.0
99	6.5
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	990441.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	74.13971974271402
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.38736765999458	64.78874947365509
2	7.1313013810416255	10.574253715825112
3	2.213281563964228	4.922762245920709
4	1.0619961062706327	3.14944374747033
5	0.6259465611947334	2.3203751310446563
6	0.3748906732090493	1.667657366752578
7	0.2613271241769808	1.3562303823355546
8	0.18098414404761398	1.0734490974052067
9	0.14299434970018837	0.9541404910407247
>10	0.6005500165026659	7.229759463781463
>50	0.014863745566001544	0.7403745731752083
>100	0.003951623585532438	0.4065241383342893
>500	0.0	0.0
>1k	5.450507461791892E-4	0.8162801732591013
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	4012	0.4050720840514478	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1575	0.1590200728766277	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1276	0.12883150031147741	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	1217	0.12287455789895613	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.0096512563595408E-4
2	0.0	0.0	0.0	0.0	1.0096512563595408E-4
3	0.0	0.0	0.0	0.0	1.0096512563595408E-4
4	0.0	0.0	0.0	0.0	1.0096512563595408E-4
5	0.0	0.0	0.0	0.0	1.0096512563595408E-4
6	0.0	0.0	0.0	0.0	1.0096512563595408E-4
7	0.0	0.0	0.0	0.0	1.0096512563595408E-4
8	1.0096512563595408E-4	0.0	0.0	0.0	1.0096512563595408E-4
9	1.0096512563595408E-4	0.0	0.0	0.0	2.0193025127190816E-4
10	1.0096512563595408E-4	0.0	0.0	0.0	2.0193025127190816E-4
11	1.0096512563595408E-4	0.0	0.0	1.0096512563595408E-4	2.0193025127190816E-4
12	1.0096512563595408E-4	0.0	0.0	1.0096512563595408E-4	2.0193025127190816E-4
13	1.0096512563595408E-4	0.0	0.0	1.0096512563595408E-4	2.0193025127190816E-4
14	1.0096512563595408E-4	0.0	0.0	1.0096512563595408E-4	3.0289537690786225E-4
15	1.0096512563595408E-4	1.0096512563595408E-4	0.0	1.0096512563595408E-4	3.0289537690786225E-4
16	1.0096512563595408E-4	1.0096512563595408E-4	0.0	2.0193025127190816E-4	5.048256281797704E-4
17	1.0096512563595408E-4	1.0096512563595408E-4	0.0	4.038605025438163E-4	5.048256281797704E-4
18	1.0096512563595408E-4	1.0096512563595408E-4	0.0	4.038605025438163E-4	5.048256281797704E-4
19	1.0096512563595408E-4	1.0096512563595408E-4	0.0	8.077210050876326E-4	5.048256281797704E-4
20	1.0096512563595408E-4	1.0096512563595408E-4	0.0	0.001110616381995495	5.048256281797704E-4
21	1.0096512563595408E-4	1.0096512563595408E-4	0.0	0.0019183373870831277	5.048256281797704E-4
22	1.0096512563595408E-4	1.0096512563595408E-4	0.0	0.0033318491459864846	5.048256281797704E-4
23	1.0096512563595408E-4	1.0096512563595408E-4	0.0	0.004139570151074117	6.057907538157245E-4
24	1.0096512563595408E-4	1.0096512563595408E-4	0.0	0.005351151658705567	6.057907538157245E-4
25	1.0096512563595408E-4	1.0096512563595408E-4	0.0	0.005654047035613429	7.067558794516786E-4
26	1.0096512563595408E-4	1.0096512563595408E-4	0.0	0.006663698291972969	7.067558794516786E-4
27	1.0096512563595408E-4	1.0096512563595408E-4	0.0	0.009995547437959454	8.077210050876326E-4
28	1.0096512563595408E-4	1.0096512563595408E-4	0.0	0.035034898595676066	8.077210050876326E-4
29	1.0096512563595408E-4	1.0096512563595408E-4	0.0	0.0912724735749025	8.077210050876326E-4
30	1.0096512563595408E-4	1.0096512563595408E-4	0.0	0.1680059690582276	8.077210050876326E-4
31	1.0096512563595408E-4	1.0096512563595408E-4	0.0	0.5000802672748805	8.077210050876326E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTATAC	565	0.0	20.955753	37
GTCTAAA	100	2.8739305E-7	18.5	1
TTATACT	110	3.850073E-8	18.5	4
GCTTAAC	130	6.9667294E-10	18.5	1
TCGCGGT	50	0.0070342114	18.5	17
GGCGTCA	145	1.546141E-10	17.862068	12
GGGCGTC	135	1.1514203E-9	17.814816	11
TCTTATA	1055	0.0	17.184835	37
GCGTCAG	140	1.8681021E-9	17.178572	13
AGGGCGT	165	5.4569682E-11	16.818182	10
GGCTTAA	80	3.3819748E-4	16.1875	1
TCTACTA	70	0.002592154	15.857142	2
ATTGGAC	70	0.002592154	15.857142	3
CGAGACG	70	0.002592154	15.857142	23
TTTATAC	155	7.203198E-9	15.516129	3
GGTATCA	1100	0.0	15.472728	1
GTGCAAC	85	5.36322E-4	15.235294	11
TACTTAC	110	1.4513858E-5	15.136364	31
GTATATA	125	2.9575585E-6	14.800001	1
ATTAGGT	75	0.004103872	14.8	3
>>END_MODULE