##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727354.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1632242
Sequences flagged as poor quality	0
Sequence length	43
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.173732203925645	33.0	31.0	34.0	30.0	34.0
2	32.316149198464444	34.0	31.0	34.0	30.0	34.0
3	32.39417868183762	34.0	31.0	34.0	30.0	34.0
4	35.957688872115774	37.0	35.0	37.0	35.0	37.0
5	35.846295463540336	37.0	35.0	37.0	35.0	37.0
6	35.86480681173502	37.0	35.0	37.0	35.0	37.0
7	35.812932763646565	37.0	35.0	37.0	35.0	37.0
8	35.789884710723044	37.0	35.0	37.0	35.0	37.0
9	37.52716631479891	39.0	37.0	39.0	35.0	39.0
10	37.366300462799025	39.0	37.0	39.0	34.0	39.0
11	37.47442781156226	39.0	37.0	39.0	35.0	39.0
12	37.380809953426024	39.0	37.0	39.0	34.0	39.0
13	37.43861326935589	39.0	37.0	39.0	35.0	39.0
14	38.60288609164572	40.0	38.0	41.0	34.0	41.0
15	38.57186311833662	40.0	38.0	41.0	34.0	41.0
16	38.5003590153911	40.0	38.0	41.0	34.0	41.0
17	38.47272463274441	40.0	38.0	41.0	34.0	41.0
18	38.42514774157264	40.0	38.0	41.0	34.0	41.0
19	38.43995498216564	40.0	38.0	41.0	34.0	41.0
20	38.318771971313076	40.0	38.0	41.0	34.0	41.0
21	38.244978992085734	40.0	38.0	41.0	34.0	41.0
22	38.17054823978307	40.0	38.0	41.0	34.0	41.0
23	38.05881848402382	40.0	38.0	41.0	33.0	41.0
24	37.94609071448964	40.0	37.0	41.0	33.0	41.0
25	37.859289860204555	40.0	37.0	41.0	33.0	41.0
26	37.49426923213592	40.0	37.0	41.0	32.0	41.0
27	37.27823141421431	39.0	37.0	41.0	31.0	41.0
28	37.11861170096101	39.0	36.0	41.0	31.0	41.0
29	37.06863810635923	39.0	36.0	40.0	31.0	41.0
30	36.97446457081732	39.0	36.0	40.0	31.0	41.0
31	36.75376690466242	39.0	36.0	40.0	30.0	41.0
32	36.633434870564535	39.0	36.0	40.0	30.0	41.0
33	36.44829933306458	39.0	35.0	40.0	30.0	41.0
34	36.30035619718154	39.0	35.0	40.0	30.0	41.0
35	36.140452825010016	39.0	35.0	40.0	29.0	41.0
36	35.967013469816365	39.0	35.0	40.0	28.0	41.0
37	35.737005909662905	38.0	35.0	40.0	27.0	41.0
38	35.62105129018859	38.0	35.0	40.0	26.0	41.0
39	35.44932246566379	38.0	35.0	40.0	26.0	41.0
40	35.30299980027471	38.0	34.0	40.0	25.0	41.0
41	35.145472301288656	38.0	34.0	40.0	24.0	41.0
42	34.93224166514524	38.0	34.0	40.0	24.0	41.0
43	31.554400021565428	35.0	29.0	38.0	14.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	1.0
10	3.0
11	5.0
12	8.0
13	11.0
14	19.0
15	22.0
16	34.0
17	87.0
18	155.0
19	321.0
20	712.0
21	1350.0
22	2372.0
23	3992.0
24	5960.0
25	8473.0
26	11712.0
27	15797.0
28	20155.0
29	25831.0
30	32687.0
31	41100.0
32	51365.0
33	64879.0
34	83111.0
35	110273.0
36	155434.0
37	246090.0
38	433806.0
39	316476.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.36816599499339	18.610353121657205	12.908073680250846	26.11340720309856
2	18.776688750810237	21.61805663620958	34.30434947758972	25.30090513539046
3	18.503567485703712	22.148799013871717	31.328810311216102	28.018823189208465
4	14.69990356822089	15.760898200144341	33.51396422834359	36.02523400329118
5	14.284585251451684	37.58241731311901	34.0159731216327	14.117024313796605
6	35.96599033721715	35.5340690902452	14.068134504564886	14.431806067972763
7	29.881169581471372	31.385848421986445	20.059954345005213	18.673027651536966
8	28.434876691078898	34.98850047970828	18.616663460442755	17.959959368770072
9	27.430246250249656	13.433547231354174	18.088923088610635	41.04728342978554
10	15.582125689695522	28.100673797145276	33.43407411401005	22.883126399149145
11	37.30721302355901	21.20157427636343	20.92355177724872	20.567660922828846
12	21.562305099366395	25.51227085199376	29.839018969000918	23.086405079638926
13	32.22971838734697	19.62729791293203	23.534132806287303	24.608850893433694
14	22.320771062134167	20.184752015938813	26.08920736018311	31.40526956174391
15	25.775099525683075	28.302727169133007	21.636987652566226	24.285185652617688
16	24.67091276906243	26.256216909012263	23.98394355738916	25.08892676453614
17	24.393074066223022	26.25058048990285	25.16220021295862	24.194145230915513
18	24.378186567923137	25.55380881021319	25.752308787544987	24.315695834318685
19	25.637681177178383	25.275234922272556	25.99577758690194	23.09130631364712
20	24.877683578783046	25.17727150753381	25.710403236774937	24.234641676908204
21	24.48546232727745	25.53922763903882	26.117757048280833	23.8575529854029
22	25.216910237575064	25.017920136842452	25.267638009559857	24.497531616022624
23	24.52877698282485	25.25520112826407	25.538860046488203	24.677161842422876
24	24.256758495370175	25.105652225589097	25.762049990136266	24.875539288904463
25	24.706262919346518	24.794607662344188	25.59369260195486	24.905436816354438
26	24.20933905634091	25.15019218963855	26.01660783143676	24.623860922583784
27	24.8281198498752	25.496096779766724	25.539656497014533	24.136126873343535
28	24.36299274249774	25.69459675709852	25.41669678883401	24.52571371156973
29	24.47835553796557	25.887889173296607	25.65814382916259	23.975611459575237
30	24.849624014086146	25.61109198268394	25.27719541587583	24.26208858735408
31	24.474189489058606	25.051799916924082	25.855234701717023	24.618775892300285
32	24.108679962897657	25.589526553047893	25.624080252805648	24.6777132312488
33	24.224471616341205	24.882401016515935	25.82006834770824	25.073059019434616
34	24.473638100232687	24.958063816517402	26.30216597783907	24.26613210541084
35	24.263252630431026	24.994026621052516	26.11469377702571	24.628026971490748
36	24.048149722896483	24.61969487367682	26.62215529314893	24.710000110277765
37	23.7734968221624	24.44521094298517	26.770233825621446	25.011058409230984
38	23.628236499244597	24.284879325492177	27.100209405223	24.986674770040228
39	23.207955683042098	24.170558042251088	27.746375843778065	24.875110430928746
40	23.63644606620832	23.7913863262923	27.917490176089082	24.654677431410292
41	23.02281156838263	23.871460236901147	28.306403094639155	24.79932510007707
42	22.58978754375883	24.111681968727677	28.24238072540714	25.056149762106354
43	22.633776118982357	23.130454920287555	28.13920974953469	26.096559211195398
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	257.0
1	280.0
2	303.0
3	738.5
4	1174.0
5	1174.0
6	1278.5
7	1383.0
8	1434.0
9	1485.0
10	2213.5
11	2942.0
12	2942.0
13	5470.5
14	7999.0
15	13073.0
16	18147.0
17	13871.5
18	9596.0
19	9596.0
20	11209.5
21	12823.0
22	11237.0
23	9651.0
24	11674.0
25	13697.0
26	13697.0
27	16539.0
28	19381.0
29	22359.5
30	25338.0
31	29744.5
32	34151.0
33	34151.0
34	39266.5
35	44382.0
36	50907.0
37	57432.0
38	64648.0
39	71864.0
40	71864.0
41	80290.0
42	88716.0
43	97537.5
44	106359.0
45	114786.5
46	123214.0
47	123214.0
48	131695.0
49	140176.0
50	143908.5
51	147641.0
52	146586.5
53	145532.0
54	145532.0
55	138332.5
56	131133.0
57	121974.0
58	112815.0
59	102937.5
60	93060.0
61	93060.0
62	81379.5
63	69699.0
64	59331.5
65	48964.0
66	40992.5
67	33021.0
68	33021.0
69	26875.0
70	20729.0
71	17260.5
72	13792.0
73	11494.5
74	9197.0
75	9197.0
76	7608.5
77	6020.0
78	5072.5
79	4125.0
80	3264.5
81	2404.0
82	2404.0
83	1978.5
84	1553.0
85	1226.5
86	900.0
87	763.5
88	627.0
89	627.0
90	482.5
91	338.0
92	237.5
93	137.0
94	97.5
95	58.0
96	58.0
97	38.0
98	18.0
99	13.5
100	9.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1632242.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	67.79700571572354
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.41069559960579	57.90589417750397
2	8.052374800914695	10.918538008055236
3	2.5039973375882183	5.092905654258749
4	1.1886657282249766	3.2235190868221366
5	0.7205374378464721	2.442514039603503
6	0.46303231289615543	1.8835322618391204
7	0.3159933255125782	1.4996380907134728
8	0.23609431323915633	1.2805190003299927
9	0.17199712387907398	1.0494800991645832
>10	0.8894090582368008	10.248545548802714
>50	0.034507271595842444	1.5577110203544524
>100	0.011970342231705674	1.2733970298230917
>500	3.6267411429096385E-4	0.16074548170942377
>1k	2.7200558571822286E-4	0.7362942179409093
>5k	0.0	0.0
>10k+	9.066852857274096E-5	0.7267662830785917
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	11823	0.7243411209857361	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	4671	0.2861708006533345	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3805	0.23311494251465165	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	3502	0.21455151870862285	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	6.126542510240516E-5
8	6.126542510240516E-5	0.0	0.0	0.0	6.126542510240516E-5
9	6.126542510240516E-5	0.0	0.0	0.0	1.8379627530721546E-4
10	1.8379627530721546E-4	0.0	0.0	0.0	1.8379627530721546E-4
11	1.8379627530721546E-4	0.0	0.0	0.0	1.8379627530721546E-4
12	1.8379627530721546E-4	6.126542510240516E-5	0.0	0.0	1.8379627530721546E-4
13	1.8379627530721546E-4	6.126542510240516E-5	0.0	0.0	1.8379627530721546E-4
14	1.8379627530721546E-4	6.126542510240516E-5	0.0	0.0	3.063271255120258E-4
15	1.8379627530721546E-4	6.126542510240516E-5	0.0	0.0	3.063271255120258E-4
16	1.8379627530721546E-4	6.126542510240516E-5	0.0	3.675925506144309E-4	4.901234008192413E-4
17	1.8379627530721546E-4	6.126542510240516E-5	0.0	6.739196761264567E-4	4.901234008192413E-4
18	1.8379627530721546E-4	6.126542510240516E-5	0.0	9.189813765360774E-4	5.513888259216464E-4
19	1.8379627530721546E-4	6.126542510240516E-5	0.0	0.001164043076945698	5.513888259216464E-4
20	1.8379627530721546E-4	6.126542510240516E-5	0.0	0.0018379627530721548	5.513888259216464E-4
21	1.8379627530721546E-4	6.126542510240516E-5	0.0	0.003124536680222663	5.513888259216464E-4
22	1.8379627530721546E-4	6.126542510240516E-5	0.0	0.005146295708602033	5.513888259216464E-4
23	1.8379627530721546E-4	6.126542510240516E-5	0.0	0.006004011660035706	6.739196761264567E-4
24	1.8379627530721546E-4	6.126542510240516E-5	0.0	0.006984258461674188	6.739196761264567E-4
25	1.8379627530721546E-4	6.126542510240516E-5	0.0	0.007535647287595835	6.739196761264567E-4
26	1.8379627530721546E-4	1.2253085020481032E-4	0.0	0.008270832388824697	6.739196761264567E-4
27	1.8379627530721546E-4	1.2253085020481032E-4	0.0	0.012743208421300273	6.739196761264567E-4
28	1.8379627530721546E-4	1.2253085020481032E-4	0.0	0.03767823643797917	6.739196761264567E-4
29	1.8379627530721546E-4	1.2253085020481032E-4	0.0	0.0974732913379266	6.739196761264567E-4
30	1.8379627530721546E-4	1.2253085020481032E-4	0.0	0.1793851646998423	6.739196761264567E-4
31	1.8379627530721546E-4	1.2253085020481032E-4	0.0	0.5227778723988232	6.739196761264567E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATACCCG	75	1.3746103E-8	24.666666	5
TACCCGT	90	9.484393E-8	20.555557	6
CTTATAC	1225	0.0	19.783674	37
ATTTCGT	65	0.0015803188	17.076923	20
GTCTAAA	190	1.8189894E-12	16.552631	1
AACGCAG	4565	0.0	16.169771	5
CGAATAC	70	0.0025931192	15.857142	4
CGAGTTA	75	0.0041053873	14.8	15
CGCAGAG	5035	0.0	14.770605	7
GGAGTGT	605	0.0	14.677686	6
ACGCAGA	5175	0.0	14.263768	6
AGAGTAC	5115	0.0	14.177908	10
AAGACCG	170	2.4383553E-8	14.147059	5
TGTACGG	105	1.656303E-4	14.095239	5
TACTTAC	370	0.0	14.0	31
ATCAACG	5340	0.0	13.96161	2
TCAACGC	5410	0.0	13.917746	3
CTATTAC	120	3.302028E-5	13.875001	1
TAATACC	160	1.7845377E-7	13.875	4
CGCAAAC	80	0.006300117	13.875	21
>>END_MODULE