FastQCFastQC Report
Thu 9 Feb 2017
SRR2727353.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727353.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2526512
Sequences flagged as poor quality0
Sequence length43
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT78000.30872602227893636No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT32700.12942744780155407No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT26060.10314615564857796No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC14800.021.62500237
GGTATCA20800.019.7451921
TATACCG1002.8783143E-718.4999985
TCTTATA27200.016.39154437
GTCTTAG3500.016.3857141
CGTATAC803.3845738E-416.18753
CGGTCGT1751.3278623E-1015.85714217
AACGCAG43700.015.4519465
ATACCGT1201.9376384E-615.4166666
TACTTAC4150.015.15662731
GTATTAT1853.0559022E-1015.0000011
TAGATCG1152.2118063E-514.4782615
TCAACGC47000.014.130853
TAGTACT2750.014.1272724
ATCAACG47250.014.0952372
GTACTTA4600.014.07608730
ACGCAGA48350.014.0423986
TATCAAC47600.014.0304611
GTATCAA28650.014.0122172
CAACGCA48150.013.9086194
Produced by FastQC (version 0.11.5)