##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727353.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2526512
Sequences flagged as poor quality	0
Sequence length	43
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.16272354930434	33.0	31.0	34.0	30.0	34.0
2	32.313331977049785	34.0	31.0	34.0	30.0	34.0
3	32.3955868802523	34.0	31.0	34.0	30.0	34.0
4	35.96618262648268	37.0	35.0	37.0	35.0	37.0
5	35.850419471587706	37.0	35.0	37.0	35.0	37.0
6	35.85891656164704	37.0	35.0	37.0	35.0	37.0
7	35.8105732329789	37.0	35.0	37.0	35.0	37.0
8	35.777993138366256	37.0	35.0	37.0	35.0	37.0
9	37.51164490807881	39.0	37.0	39.0	35.0	39.0
10	37.3448022411926	39.0	37.0	39.0	34.0	39.0
11	37.45313143179213	39.0	37.0	39.0	35.0	39.0
12	37.360306224549895	39.0	37.0	39.0	34.0	39.0
13	37.420765466382115	39.0	37.0	39.0	35.0	39.0
14	38.60278993331518	40.0	38.0	41.0	34.0	41.0
15	38.55956156155205	40.0	38.0	41.0	34.0	41.0
16	38.48500739359243	40.0	38.0	41.0	34.0	41.0
17	38.44475189510281	40.0	38.0	41.0	34.0	41.0
18	38.384721703281045	40.0	38.0	41.0	34.0	41.0
19	38.392581551166195	40.0	38.0	41.0	34.0	41.0
20	38.26908797583388	40.0	38.0	41.0	34.0	41.0
21	38.18988233580525	40.0	38.0	41.0	34.0	41.0
22	38.11634854692952	40.0	38.0	41.0	33.0	41.0
23	38.00475794296643	40.0	37.0	41.0	33.0	41.0
24	37.88826136586725	40.0	37.0	41.0	33.0	41.0
25	37.80230412521294	40.0	37.0	41.0	33.0	41.0
26	37.442650974940946	39.0	37.0	41.0	32.0	41.0
27	37.22553267112921	39.0	37.0	41.0	31.0	41.0
28	37.05341712210352	39.0	36.0	41.0	31.0	41.0
29	37.01826747705928	39.0	36.0	40.0	31.0	41.0
30	36.93629715592089	39.0	36.0	40.0	30.0	41.0
31	36.72731061637546	39.0	36.0	40.0	30.0	41.0
32	36.62335900245081	39.0	36.0	40.0	30.0	41.0
33	36.44129574686366	39.0	36.0	40.0	30.0	41.0
34	36.29878900238748	39.0	35.0	40.0	29.0	41.0
35	36.14390511503606	39.0	35.0	40.0	29.0	41.0
36	35.98803370021595	39.0	35.0	40.0	27.0	41.0
37	35.76642264117487	38.0	35.0	40.0	27.0	41.0
38	35.65985952174381	38.0	35.0	40.0	26.0	41.0
39	35.50200276111889	38.0	35.0	40.0	26.0	41.0
40	35.373378792580446	38.0	34.0	40.0	25.0	41.0
41	35.238250204234134	38.0	34.0	40.0	25.0	41.0
42	35.04975832293692	38.0	34.0	40.0	24.0	41.0
43	31.57231748750847	35.0	29.0	38.0	14.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	2.0
11	7.0
12	3.0
13	4.0
14	16.0
15	29.0
16	61.0
17	115.0
18	257.0
19	541.0
20	1114.0
21	2061.0
22	3769.0
23	6200.0
24	9506.0
25	13387.0
26	18506.0
27	24233.0
28	31926.0
29	40562.0
30	51041.0
31	64026.0
32	79766.0
33	100992.0
34	129482.0
35	170780.0
36	239361.0
37	372653.0
38	676468.0
39	489642.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.43363973731373	18.491422166211755	12.36665410653106	26.70828398994345
2	18.83588916260837	20.92287707321398	34.60169593494905	25.639537829228598
3	18.292610523915975	21.725643891657747	31.08835422115549	28.893391363270787
4	14.674064480991976	15.536043367298474	33.873221263148565	35.916670888560986
5	14.28526759421685	37.23473310239571	34.321388538823484	14.158610764563953
6	36.35011430778876	35.74152824130659	13.971989842122262	13.936367608782385
7	30.55473316572413	31.429258994218117	19.658525271203935	18.35748256885382
8	28.192979095290266	35.31421976226513	18.454058401464152	18.038742740980453
9	27.747622018023264	13.3707261236044	17.577474399488306	41.30417745888403
10	15.43772600328041	27.63600568689165	33.7547971274231	23.171471182404833
11	37.903916545814944	20.831684155863893	20.985295142077298	20.279104156243864
12	21.917568568841155	24.912685948058034	29.72758490757218	23.442160575528632
13	32.127296446642646	19.362029549038358	23.22660648356311	25.284067520755887
14	22.573373884628296	19.801014204563447	25.24262698930383	32.38298492150443
15	25.929265327059596	27.862602671192537	21.15101768762626	25.057114314121602
16	25.219076735040247	26.26375809812105	23.51475077102345	25.002414395815258
17	24.984286637071186	26.009573673111387	24.475363663422144	24.530776026395284
18	24.821888833300616	25.66993546834529	24.936513264136487	24.571662434217608
19	26.056317959305158	25.23807525948818	25.02865610770897	23.67695067349769
20	25.436134876857896	25.01777153641067	24.71320144135472	24.832892145376707
21	25.221491130855505	25.187650009182622	25.148346811730953	24.44251204823092
22	25.89768028016491	24.76695143343867	24.328718802839646	25.006649483556775
23	25.076231579347336	25.01496133800275	24.690086569943066	25.218720512706845
24	24.938056894247882	24.81417068274364	24.783773043626944	25.463999379381537
25	25.31395061650212	24.261234460790213	24.74427194487895	25.680542977828726
26	24.92333303780073	24.731210459320994	25.01092415155756	25.334532351320714
27	25.513514283723964	25.049198262268295	24.554484601695936	24.8828028523118
28	24.957847023881143	25.347950059212067	24.564933790142298	25.129269126764488
29	25.123371668133775	25.57870297073594	24.668198686568676	24.62972667456161
30	25.621330909965994	25.27607230838405	24.263965498679603	24.838631282970354
31	25.205579866630355	24.629291291709677	24.80791700177953	25.357211839880435
32	24.756502244992305	25.09994015464799	24.688463779313143	25.455093821046564
33	24.847853483379456	24.44389735730525	24.955828430658553	25.752420728656737
34	25.045161075823113	24.43693123167434	25.641754323747524	24.876153368755027
35	24.93643410361795	24.351319130880835	25.400750125073618	25.311496640427595
36	24.651456236898934	24.11443127917065	25.830433419671074	25.403679064259343
37	24.397271811889276	23.735529457212156	26.052676575452637	25.81452215544593
38	24.320842331245608	23.484115650351157	26.333419354430138	25.8616226639731
39	23.837844427416137	23.442595958380565	27.044162070079224	25.675397544124074
40	24.20249735603868	23.048970279974924	27.393655759402684	25.35487660458371
41	23.579424914664962	23.09804980146542	27.820568435851484	25.501956848018136
42	23.0266470137486	23.31890764817266	27.904122363163125	25.750322974915612
43	23.09793106068762	22.21018542559861	27.781106917362752	26.910776596351017
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	129.0
1	170.0
2	211.0
3	485.0
4	759.0
5	759.0
6	859.5
7	960.0
8	1021.5
9	1083.0
10	1613.0
11	2143.0
12	2143.0
13	3824.5
14	5506.0
15	9050.5
16	12595.0
17	10338.5
18	8082.0
19	8082.0
20	9762.5
21	11443.0
22	11572.5
23	11702.0
24	14551.5
25	17401.0
26	17401.0
27	21337.0
28	25273.0
29	30044.5
30	34816.0
31	41521.0
32	48226.0
33	48226.0
34	56056.5
35	63887.0
36	74333.0
37	84779.0
38	97024.5
39	109270.0
40	109270.0
41	123194.0
42	137118.0
43	152447.5
44	167777.0
45	182471.0
46	197165.0
47	197165.0
48	211430.5
49	225696.0
50	233470.0
51	241244.0
52	238279.0
53	235314.0
54	235314.0
55	224950.0
56	214586.0
57	199130.5
58	183675.0
59	166934.0
60	150193.0
61	150193.0
62	131382.0
63	112571.0
64	95794.5
65	79018.0
66	65432.5
67	51847.0
68	51847.0
69	42329.0
70	32811.0
71	26978.5
72	21146.0
73	17457.0
74	13768.0
75	13768.0
76	11433.5
77	9099.0
78	7570.0
79	6041.0
80	4855.0
81	3669.0
82	3669.0
83	2969.5
84	2270.0
85	1817.0
86	1364.0
87	1179.5
88	995.0
89	995.0
90	756.5
91	518.0
92	371.5
93	225.0
94	161.5
95	98.0
96	98.0
97	63.0
98	28.0
99	19.5
100	11.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	2526512.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	65.54897437187302
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.39535945480256	55.32029254007864
2	8.291151956216236	10.869530141826457
3	2.7905802312838417	5.4875901618924
4	1.3070586626753338	3.4270541912896024
5	0.8043792411065086	2.636311713027859
6	0.5072532206681208	1.9949957016974817
7	0.346280449367094	1.588882981073102
8	0.26966241833444565	1.41408759587695
9	0.18887266124685026	1.1142368308455517
>10	1.0419998408543392	11.959126496089521
>50	0.04364896312792539	1.9293017468990634
>100	0.013329958892556408	1.534296883958499
>500	1.8126061037011983E-4	0.09109109446226468
>1k	1.8126061036966647E-4	0.3242842962850529
>5k	6.042020345655549E-5	0.3089176246975345
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	7800	0.30872602227893636	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	3270	0.12942744780155407	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	2606	0.10314615564857796	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	1.1874077779959089E-4
7	0.0	0.0	0.0	0.0	1.1874077779959089E-4
8	0.0	0.0	0.0	0.0	1.583210370661212E-4
9	0.0	0.0	0.0	3.95802592665303E-5	3.166420741322424E-4
10	3.95802592665303E-5	0.0	0.0	7.91605185330606E-5	3.166420741322424E-4
11	3.95802592665303E-5	0.0	0.0	7.91605185330606E-5	3.166420741322424E-4
12	3.95802592665303E-5	0.0	0.0	7.91605185330606E-5	3.562223333987727E-4
13	3.95802592665303E-5	7.91605185330606E-5	0.0	7.91605185330606E-5	3.562223333987727E-4
14	3.95802592665303E-5	7.91605185330606E-5	0.0	7.91605185330606E-5	4.353828519318333E-4
15	3.95802592665303E-5	7.91605185330606E-5	0.0	7.91605185330606E-5	4.353828519318333E-4
16	3.95802592665303E-5	7.91605185330606E-5	0.0	3.166420741322424E-4	4.7496311119836357E-4
17	3.95802592665303E-5	7.91605185330606E-5	0.0	4.7496311119836357E-4	5.145433704648939E-4
18	3.95802592665303E-5	7.91605185330606E-5	0.0	5.541236297314242E-4	5.541236297314242E-4
19	3.95802592665303E-5	7.91605185330606E-5	0.0	7.124446667975454E-4	5.541236297314242E-4
20	3.95802592665303E-5	7.91605185330606E-5	0.0	0.0011082472594628485	5.541236297314242E-4
21	3.95802592665303E-5	7.91605185330606E-5	0.0	0.0017019511484608028	5.541236297314242E-4
22	3.95802592665303E-5	7.91605185330606E-5	0.0	0.003166420741322424	5.541236297314242E-4
23	3.95802592665303E-5	7.91605185330606E-5	0.0	0.003839285148853439	7.91605185330606E-4
24	3.95802592665303E-5	7.91605185330606E-5	0.0	0.004789211371250166	7.91605185330606E-4
25	3.95802592665303E-5	7.91605185330606E-5	0.0	0.00526417448244853	8.311854445971363E-4
26	3.95802592665303E-5	7.91605185330606E-5	0.0	0.0060161994085126055	8.311854445971363E-4
27	3.95802592665303E-5	7.91605185330606E-5	0.0	0.010211706890764817	8.707657038636666E-4
28	3.95802592665303E-5	7.91605185330606E-5	0.0	0.03415776374701565	8.707657038636666E-4
29	3.95802592665303E-5	1.1874077779959089E-4	0.0	0.0896097069794246	8.707657038636666E-4
30	3.95802592665303E-5	1.1874077779959089E-4	0.0	0.16461429828949953	8.707657038636666E-4
31	3.95802592665303E-5	1.1874077779959089E-4	0.0	0.5484240723970438	9.103459631301969E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTATAC	1480	0.0	21.625002	37
GGTATCA	2080	0.0	19.745192	1
TATACCG	100	2.8783143E-7	18.499998	5
TCTTATA	2720	0.0	16.391544	37
GTCTTAG	350	0.0	16.385714	1
CGTATAC	80	3.3845738E-4	16.1875	3
CGGTCGT	175	1.3278623E-10	15.857142	17
AACGCAG	4370	0.0	15.451946	5
ATACCGT	120	1.9376384E-6	15.416666	6
TACTTAC	415	0.0	15.156627	31
GTATTAT	185	3.0559022E-10	15.000001	1
TAGATCG	115	2.2118063E-5	14.478261	5
TCAACGC	4700	0.0	14.13085	3
TAGTACT	275	0.0	14.127272	4
ATCAACG	4725	0.0	14.095237	2
GTACTTA	460	0.0	14.076087	30
ACGCAGA	4835	0.0	14.042398	6
TATCAAC	4760	0.0	14.030461	1
GTATCAA	2865	0.0	14.012217	2
CAACGCA	4815	0.0	13.908619	4
>>END_MODULE