FastQCFastQC Report
Thu 9 Feb 2017
SRR2727352.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727352.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2187939
Sequences flagged as poor quality0
Sequence length43
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT51520.23547274398417872No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT25530.11668515438501713No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGATCG1059.840733E-1021.1428575
CTTATAC10000.020.90499937
GGTATCA16750.019.1074621
AACGACG1306.9667294E-1018.50000215
CTACACT3700.016.54
TCTTATA18000.015.82777837
TACTTAC5000.015.5431
GCGACGT1508.1112375E-814.835
ATACCGT750.004105982414.86
TAGACAG3400.014.6911755
GTACTTA5700.014.60526330
AACGCAG35750.014.5930075
CGCAATC1406.0014827E-714.53571522
CGCGCAA1152.211538E-514.47826120
CGCGGTT908.278226E-414.38888918
TAAGAGT3650.014.1917814
CATTACG4200.014.09523824
TCAACGC36850.014.0569883
CTAGACA2900.014.0344834
CAACGCA37400.013.8997324
Produced by FastQC (version 0.11.5)