##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727352.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2187939
Sequences flagged as poor quality	0
Sequence length	43
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.18383236461346	33.0	31.0	34.0	30.0	34.0
2	32.330058104910606	34.0	31.0	34.0	30.0	34.0
3	32.407743543124376	34.0	31.0	34.0	30.0	34.0
4	35.97999395778402	37.0	35.0	37.0	35.0	37.0
5	35.85608830959181	37.0	35.0	37.0	35.0	37.0
6	35.865486194999036	37.0	35.0	37.0	35.0	37.0
7	35.81390386112227	37.0	35.0	37.0	35.0	37.0
8	35.788530667445485	37.0	35.0	37.0	35.0	37.0
9	37.51723608382135	39.0	37.0	39.0	35.0	39.0
10	37.35679742442545	39.0	37.0	39.0	34.0	39.0
11	37.466348467667515	39.0	37.0	39.0	35.0	39.0
12	37.36927583447253	39.0	37.0	39.0	34.0	39.0
13	37.42991920707113	39.0	37.0	39.0	35.0	39.0
14	38.607531105757516	40.0	38.0	41.0	34.0	41.0
15	38.573482624515584	40.0	38.0	41.0	34.0	41.0
16	38.50219133165961	40.0	38.0	41.0	34.0	41.0
17	38.45851963880163	40.0	38.0	41.0	34.0	41.0
18	38.39450871345134	40.0	38.0	41.0	34.0	41.0
19	38.403354024038144	40.0	38.0	41.0	34.0	41.0
20	38.274597235114875	40.0	38.0	41.0	34.0	41.0
21	38.19350036724059	40.0	38.0	41.0	34.0	41.0
22	38.12507844140079	40.0	38.0	41.0	33.0	41.0
23	38.00797919868881	40.0	38.0	41.0	33.0	41.0
24	37.902674617528184	40.0	37.0	41.0	33.0	41.0
25	37.800772325005404	40.0	37.0	41.0	33.0	41.0
26	37.438510854278846	39.0	37.0	41.0	32.0	41.0
27	37.22640165013741	39.0	37.0	41.0	31.0	41.0
28	37.047950148518765	39.0	36.0	41.0	31.0	41.0
29	37.025345313557644	39.0	36.0	40.0	31.0	41.0
30	36.94886511918294	39.0	36.0	40.0	30.0	41.0
31	36.740153176116884	39.0	36.0	40.0	30.0	41.0
32	36.62511523401704	39.0	36.0	40.0	30.0	41.0
33	36.4488557496347	39.0	36.0	40.0	30.0	41.0
34	36.30451260295648	39.0	35.0	40.0	29.0	41.0
35	36.14473986706211	39.0	35.0	40.0	29.0	41.0
36	35.9824515217289	39.0	35.0	40.0	27.0	41.0
37	35.75550460958921	38.0	35.0	40.0	27.0	41.0
38	35.643598381856165	38.0	35.0	40.0	26.0	41.0
39	35.46891115337311	38.0	35.0	40.0	26.0	41.0
40	35.35048052070921	38.0	34.0	40.0	25.0	41.0
41	35.211047931409425	38.0	34.0	40.0	25.0	41.0
42	35.02812464150052	38.0	34.0	40.0	24.0	41.0
43	31.533410666385123	35.0	28.0	38.0	14.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	3.0
11	1.0
12	4.0
13	7.0
14	15.0
15	24.0
16	57.0
17	126.0
18	224.0
19	462.0
20	969.0
21	1875.0
22	3332.0
23	5419.0
24	8363.0
25	11908.0
26	16110.0
27	21313.0
28	27535.0
29	34842.0
30	43834.0
31	54947.0
32	69242.0
33	86544.0
34	111152.0
35	147530.0
36	206160.0
37	321801.0
38	592129.0
39	422010.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.61023730551903	18.11161097270079	12.352401049572222	26.925750672207954
2	19.341124226955138	20.68010122768505	34.13335563742865	25.845418907931162
3	18.73288057848048	21.54753857397304	30.675900927768097	29.043679919778388
4	15.438318892802771	15.42364755141711	32.81508305304672	36.32295050273339
5	14.72413079158057	36.79078804299389	33.909400582008914	14.57568058341663
6	37.05898564813735	34.404432664713234	14.005143653456518	14.531438033692895
7	30.53051296219867	30.998487617799213	19.676234118044427	18.794765301957685
8	28.196398528478174	34.8996932729843	18.53241795132314	18.371490247214386
9	27.944974699934505	13.322903426466642	17.46735169490557	41.26477017869328
10	15.726306812027211	27.38115642163698	33.5092523146212	23.3832844517146
11	38.11596209949181	20.559759664231954	20.58375484874121	20.740523387535028
12	21.940739664131403	24.447710836545262	29.395609292580826	24.21594020674251
13	32.25195035144947	19.434134132624354	22.84574661359389	25.468168902332287
14	22.761923435708216	19.828477850616494	24.85306948685498	32.55652922682031
15	26.353339832600454	27.63349435244767	20.893681222374116	25.119484592577763
16	25.46327845520373	25.963749446396818	23.287760764811086	25.28521133358837
17	25.168343358749944	25.559259193240763	24.08673185129933	25.18566559670996
18	24.952752339073438	25.594360720294308	24.59661809584271	24.856268844789547
19	26.448589288823865	25.00097123365871	24.63030276438237	23.920136713135058
20	25.638329039337933	24.605439182719447	24.459502755789806	25.296729022152814
21	25.308429531170656	24.979032779250243	25.030222506203327	24.68231518337577
22	26.142684965165845	24.353238367248814	23.96620746739283	25.53786920019251
23	25.322689526536163	24.710378122973264	24.467821086419686	25.49911126407089
24	25.074510761040415	24.329288887852908	24.638986735919055	25.957213615187623
25	25.586408030571235	24.060679936689276	24.428057637804347	25.924854394935142
26	25.217156419808777	24.500500242465627	24.77779316516594	25.504550172559654
27	25.91242260410368	24.86760371290059	24.113515047722995	25.106458635272737
28	25.142885610613458	25.36528669217926	23.829274947793333	25.662552749413948
29	25.36821181943372	25.327351448097957	24.392133418710486	24.912303313757832
30	26.183819567181715	24.73281933362859	23.873426087290365	25.209935011899326
31	25.608117959412947	24.257257629211786	24.662296343728045	25.472328067647226
32	25.216013791975005	24.805079117836463	24.287925760270284	25.69098132991825
33	25.21071199882629	24.05853179636178	24.56631560569102	26.16444059912091
34	25.54074862233362	24.257349039438488	25.030588147110134	25.171314191117762
35	25.43155910653816	24.092810631375006	24.80096565763488	25.67466460445195
36	25.154266183837848	23.788780217364376	25.3741991892827	25.682754409515073
37	24.776604832218815	23.743669270486976	25.718587218382233	25.761138678911983
38	24.94493676469042	23.392973935745008	25.725305870044824	25.936783429519743
39	24.133990938504226	23.64878545517037	26.519386509404512	25.697837096920896
40	24.990321942247935	22.966225292387037	26.51833529179744	25.525117473567587
41	24.1634707366156	23.295987685214257	26.828673011450505	25.711868566719637
42	23.80198899512281	23.408925020304498	26.866608255531805	25.922477729040892
43	23.938144527795334	22.521560244595484	26.5636747642416	26.976620463367578
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	167.0
1	195.0
2	223.0
3	425.5
4	628.0
5	628.0
6	714.5
7	801.0
8	844.5
9	888.0
10	1261.0
11	1634.0
12	1634.0
13	2907.5
14	4181.0
15	6372.5
16	8564.0
17	7125.0
18	5686.0
19	5686.0
20	7176.5
21	8667.0
22	8688.5
23	8710.0
24	10961.0
25	13212.0
26	13212.0
27	16098.0
28	18984.0
29	22763.0
30	26542.0
31	32188.5
32	37835.0
33	37835.0
34	44358.5
35	50882.0
36	59472.0
37	68062.0
38	77855.5
39	87649.0
40	87649.0
41	99459.0
42	111269.0
43	124559.0
44	137849.0
45	152670.5
46	167492.0
47	167492.0
48	181185.5
49	194879.0
50	204370.0
51	213861.0
52	212803.0
53	211745.0
54	211745.0
55	201240.5
56	190736.0
57	178576.0
58	166416.0
59	151566.0
60	136716.0
61	136716.0
62	120037.0
63	103358.0
64	88367.5
65	73377.0
66	60956.0
67	48535.0
68	48535.0
69	40116.0
70	31697.0
71	26092.5
72	20488.0
73	16888.5
74	13289.0
75	13289.0
76	11001.5
77	8714.0
78	7274.0
79	5834.0
80	4680.5
81	3527.0
82	3527.0
83	2822.5
84	2118.0
85	1660.0
86	1202.0
87	995.0
88	788.0
89	788.0
90	609.5
91	431.0
92	316.0
93	201.0
94	135.5
95	70.0
96	70.0
97	46.0
98	22.0
99	16.0
100	10.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	2187939.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	65.87936400183291
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.9967994236995	55.336557242228466
2	8.653002871990333	11.401086518255134
3	2.819457170743542	5.572321357169752
4	1.318597210774444	3.474733824816447
5	0.7956656939249687	2.6208974936926985
6	0.4962116973111379	1.9614066617476666
7	0.3740618347785143	1.7250069040797054
8	0.2586146174672799	1.3629893216257363
9	0.21267553446397597	1.2609836054312966
>10	1.0232629040730774	11.507606898033204
>50	0.037038948828518076	1.609324620226914
>100	0.014125739382187967	1.5520433975766925
>500	2.0843681249929554E-4	0.09722032726552199
>1k	2.0843681249929516E-4	0.28200303026500945
>5k	6.947893749976505E-5	0.23581879758567256
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	5152	0.23547274398417872	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	2553	0.11668515438501713	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	9.141022670193273E-5
7	4.5705113350966366E-5	0.0	0.0	0.0	1.371153400528991E-4
8	4.5705113350966366E-5	0.0	0.0	0.0	1.371153400528991E-4
9	4.5705113350966366E-5	0.0	0.0	4.5705113350966366E-5	1.8282045340386547E-4
10	4.5705113350966366E-5	0.0	0.0	4.5705113350966366E-5	1.8282045340386547E-4
11	1.371153400528991E-4	0.0	0.0	4.5705113350966366E-5	1.8282045340386547E-4
12	1.371153400528991E-4	0.0	0.0	4.5705113350966366E-5	1.8282045340386547E-4
13	1.371153400528991E-4	0.0	0.0	4.5705113350966366E-5	2.2852556675483183E-4
14	1.371153400528991E-4	0.0	0.0	4.5705113350966366E-5	3.1993579345676457E-4
15	1.371153400528991E-4	0.0	0.0	4.5705113350966366E-5	3.6564090680773093E-4
16	1.371153400528991E-4	0.0	0.0	1.8282045340386547E-4	4.5705113350966365E-4
17	1.371153400528991E-4	0.0	0.0	2.2852556675483183E-4	4.5705113350966365E-4
18	1.371153400528991E-4	0.0	0.0	3.1993579345676457E-4	5.027562468606301E-4
19	1.8282045340386547E-4	0.0	0.0	3.6564090680773093E-4	5.027562468606301E-4
20	1.8282045340386547E-4	0.0	0.0	5.027562468606301E-4	5.027562468606301E-4
21	1.8282045340386547E-4	0.0	0.0	5.941664735625628E-4	5.027562468606301E-4
22	1.8282045340386547E-4	0.0	0.0	0.0012340380604760918	5.027562468606301E-4
23	1.8282045340386547E-4	0.0	0.0	0.0014625636272309237	0.0010969227204231928
24	1.8282045340386547E-4	0.0	0.0	0.0018282045340386546	0.0011883329471251256
25	1.8282045340386547E-4	0.0	0.0	0.0020567301007934865	0.0012797431738270583
26	1.8282045340386547E-4	0.0	0.0	0.0024223710076012174	0.0012797431738270583
27	1.8282045340386547E-4	0.0	0.0	0.005621728942168863	0.0012797431738270583
28	1.8282045340386547E-4	0.0	0.0	0.024680761209521836	0.0012797431738270583
29	1.8282045340386547E-4	0.0	0.0	0.06353010755784325	0.0012797431738270583
30	1.8282045340386547E-4	0.0	0.0	0.11979310209288284	0.0012797431738270583
31	1.8282045340386547E-4	0.0	0.0	0.3751932754980829	0.0012797431738270583
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGATCG	105	9.840733E-10	21.142857	5
CTTATAC	1000	0.0	20.904999	37
GGTATCA	1675	0.0	19.107462	1
AACGACG	130	6.9667294E-10	18.500002	15
CTACACT	370	0.0	16.5	4
TCTTATA	1800	0.0	15.827778	37
TACTTAC	500	0.0	15.54	31
GCGACGT	150	8.1112375E-8	14.8	35
ATACCGT	75	0.0041059824	14.8	6
TAGACAG	340	0.0	14.691175	5
GTACTTA	570	0.0	14.605263	30
AACGCAG	3575	0.0	14.593007	5
CGCAATC	140	6.0014827E-7	14.535715	22
CGCGCAA	115	2.211538E-5	14.478261	20
CGCGGTT	90	8.278226E-4	14.388889	18
TAAGAGT	365	0.0	14.191781	4
CATTACG	420	0.0	14.095238	24
TCAACGC	3685	0.0	14.056988	3
CTAGACA	290	0.0	14.034483	4
CAACGCA	3740	0.0	13.899732	4
>>END_MODULE