FastQCFastQC Report
Thu 9 Feb 2017
SRR2727351.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727351.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3355189
Sequences flagged as poor quality0
Sequence length43
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT95210.283769409115254No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT50520.1505727397174943No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33910.10106733182542027No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC16400.018.95121837
TCTTATA30500.015.89180437
GGTATCA25950.015.2562621
AACGCAG47450.013.8798735
GTATTAG3200.013.8750011
GCGCTTA800.006301943713.87500113
TCGCGTA950.001245945813.631577523
CTCTTAT49350.013.45795437
CTAATAC3200.013.2968763
TATACTG5650.012.4424775
TCAACGC52200.012.4396553
CAACGCA52150.012.4161084
TATCAAC52400.012.3921761
ATCAACG52100.012.3570052
GTATAAG4350.012.3333331
ACGCAGA55550.011.9558956
TAGACAG6200.011.9354835
CGCGAAT1401.3998397E-411.89285828
TTATACG1401.3998397E-411.8928584
ATCGCGT1100.003747615511.77272722
Produced by FastQC (version 0.11.5)