##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727351.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3355189
Sequences flagged as poor quality	0
Sequence length	43
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.07058976409377	33.0	31.0	34.0	30.0	34.0
2	32.22756035502024	33.0	31.0	34.0	30.0	34.0
3	32.309335480057904	34.0	31.0	34.0	30.0	34.0
4	35.9010037884602	37.0	35.0	37.0	35.0	37.0
5	35.76942193122355	37.0	35.0	37.0	35.0	37.0
6	35.78431796241583	37.0	35.0	37.0	35.0	37.0
7	35.73487723046302	37.0	35.0	37.0	33.0	37.0
8	35.705531640691476	37.0	35.0	37.0	33.0	37.0
9	37.42302534968969	39.0	37.0	39.0	35.0	39.0
10	37.25729936525185	39.0	37.0	39.0	34.0	39.0
11	37.36150959007078	39.0	37.0	39.0	34.0	39.0
12	37.260844620079524	39.0	37.0	39.0	34.0	39.0
13	37.32142034323551	39.0	37.0	39.0	34.0	39.0
14	38.49766823865958	40.0	38.0	41.0	34.0	41.0
15	38.455946296915016	40.0	38.0	41.0	34.0	41.0
16	38.392442571789545	40.0	38.0	41.0	34.0	41.0
17	38.345500357803985	40.0	38.0	41.0	34.0	41.0
18	38.29342043026488	40.0	38.0	41.0	33.0	41.0
19	38.29483137909668	40.0	38.0	41.0	34.0	41.0
20	38.17849098813807	40.0	38.0	41.0	34.0	41.0
21	38.10589776015598	40.0	38.0	41.0	33.0	41.0
22	38.03002573029418	40.0	37.0	41.0	33.0	41.0
23	37.91596598582077	40.0	37.0	41.0	33.0	41.0
24	37.81172476423832	40.0	37.0	41.0	33.0	41.0
25	37.731233024428725	40.0	37.0	41.0	32.0	41.0
26	37.37890533141352	39.0	37.0	41.0	32.0	41.0
27	37.1712234392757	39.0	36.0	41.0	31.0	41.0
28	37.02989459014082	39.0	36.0	40.0	31.0	41.0
29	36.99523007496746	39.0	36.0	40.0	31.0	41.0
30	36.92561164214594	39.0	36.0	40.0	30.0	41.0
31	36.728418279864414	39.0	36.0	40.0	30.0	41.0
32	36.62589976302378	39.0	36.0	40.0	30.0	41.0
33	36.44963845553857	39.0	36.0	40.0	30.0	41.0
34	36.32780388824594	39.0	35.0	40.0	30.0	41.0
35	36.18180734378898	39.0	35.0	40.0	29.0	41.0
36	36.03762410999798	38.0	35.0	40.0	28.0	41.0
37	35.80511381028013	38.0	35.0	40.0	27.0	41.0
38	35.71079691784874	38.0	35.0	40.0	27.0	41.0
39	35.55399919348806	38.0	35.0	40.0	26.0	41.0
40	35.42813147038811	38.0	34.0	40.0	26.0	41.0
41	35.31038013059771	38.0	34.0	40.0	25.0	41.0
42	35.124957789263135	38.0	34.0	40.0	25.0	41.0
43	31.722984308782603	35.0	29.0	38.0	15.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	0.0
9	3.0
10	5.0
11	7.0
12	6.0
13	9.0
14	16.0
15	38.0
16	62.0
17	133.0
18	292.0
19	667.0
20	1401.0
21	2617.0
22	4980.0
23	8101.0
24	12385.0
25	18096.0
26	24127.0
27	32705.0
28	42789.0
29	54669.0
30	68985.0
31	86708.0
32	108472.0
33	136300.0
34	175171.0
35	230407.0
36	322099.0
37	506302.0
38	912141.0
39	605495.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.16164871785166	18.987365540361512	12.44409778405926	26.406887957727566
2	18.95368636461314	20.925497788649164	34.28292713167574	25.83788871506195
3	18.96677057536848	21.99664460034889	30.340734903458493	28.695849920824134
4	15.209307135902032	15.834011139163845	33.1024869239855	35.85419480094862
5	14.862858694398437	36.5118328654511	33.92261956032879	14.702688879821673
6	36.687858716751876	34.75968119828719	14.148442904408665	14.404017180552273
7	30.323239614817528	30.613595836180913	20.00924538081163	19.05391916818993
8	27.79059540312036	34.83502717730656	18.669440082212954	18.704937337360132
9	26.732592411336594	13.746915598495345	18.380335653222517	41.14015633694555
10	15.819824158937095	27.61981515795384	33.890609441077686	22.66975124203137
11	37.300670692470675	20.933545025332403	21.081882421526778	20.683901860670144
12	21.645039966451964	24.80852792495445	30.018100321621226	23.52833178697236
13	31.704532889205346	19.8057099018863	23.357939001349852	25.131818207558503
14	22.613033125704693	20.11311434318603	25.186241371201447	32.08761115990783
15	25.540439003585192	27.921765361057155	21.499981074091504	25.037814561266146
16	25.340360855975625	26.212740921599348	23.574469277289595	24.87242894513543
17	24.531285718926714	26.360869685731565	24.89514003533035	24.21270456001137
18	24.628597673633287	25.724035218284275	25.16898451920294	24.478382588879494
19	25.261766177702654	25.61813954444891	25.32176279786325	23.798331479985183
20	25.29627988169966	25.21857934083594	25.07852761796727	24.406613159497127
21	24.994478701497886	25.178760421544062	25.085412476018487	24.741348400939557
22	25.483661278097898	25.01802431994144	24.743404916980833	24.754909484979834
23	25.117780250233295	25.03429762078977	24.72179659625732	25.126125532719616
24	25.001482777870336	25.046904958260175	24.806858868457187	25.1447533954123
25	25.237386031010473	24.723406043593968	24.954749195946935	25.08445872944862
26	24.912158450686384	25.0746828271075	25.022107547443678	24.991051174762436
27	25.029171232976744	25.293210009927908	24.906435971267193	24.77118278582816
28	24.978980319737577	25.282003487731984	24.90154802009663	24.837468172433802
29	24.932187128653556	25.467000517705564	25.055607895710196	24.545204457930687
30	25.13077504724771	25.240455902782227	24.897256160532237	24.731512889437823
31	25.117273572368052	24.818214413554646	25.02204793828306	25.04246407579424
32	24.748412086472623	25.009589623714195	25.085233648536644	25.156764641276542
33	24.88014833143528	24.762062584253822	24.999575284730607	25.35821379958029
34	24.990514692316886	24.53167317847072	25.34322209568522	25.13459003352717
35	24.85582779390371	24.54287970066664	25.41829983348181	25.18299267194784
36	24.697476058725755	24.348881687439963	25.726687825931716	25.22695442790257
37	24.43951741615748	23.900054512577384	26.10678563860337	25.553642432661768
38	24.037036363674297	23.87913169720096	26.589947689981102	25.49388424914364
39	23.88163528194686	23.72384983379476	27.006705136431957	25.387809747826427
40	23.975519709917982	23.64197665168788	27.267554823290137	25.11494881510401
41	23.686564303829083	23.551787991674985	27.480001871727644	25.28164583276829
42	23.233713510624888	23.744325580466555	27.525751902500872	25.49620900640769
43	23.574946150574526	23.123943241349444	27.30042927537018	26.00068133270585
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	620.0
1	559.5
2	499.0
3	832.5
4	1166.0
5	1166.0
6	1313.0
7	1460.0
8	1491.5
9	1523.0
10	2374.5
11	3226.0
12	3226.0
13	5695.0
14	8164.0
15	12379.0
16	16594.0
17	13950.0
18	11306.0
19	11306.0
20	14167.5
21	17029.0
22	17129.0
23	17229.0
24	21223.0
25	25217.0
26	25217.0
27	30742.0
28	36267.0
29	42719.5
30	49172.0
31	57917.5
32	66663.0
33	66663.0
34	78360.5
35	90058.0
36	103550.0
37	117042.0
38	132706.5
39	148371.0
40	148371.0
41	166859.0
42	185347.0
43	204417.5
44	223488.0
45	240901.0
46	258314.0
47	258314.0
48	274624.0
49	290934.0
50	297783.0
51	304632.0
52	304854.0
53	305076.0
54	305076.0
55	294235.5
56	283395.0
57	263963.0
58	244531.0
59	222564.5
60	200598.0
61	200598.0
62	175860.0
63	151122.0
64	128923.5
65	106725.0
66	88665.0
67	70605.0
68	70605.0
69	57840.5
70	45076.0
71	36894.0
72	28712.0
73	23239.5
74	17767.0
75	17767.0
76	14589.0
77	11411.0
78	9206.5
79	7002.0
80	5561.0
81	4120.0
82	4120.0
83	3194.5
84	2269.0
85	1765.0
86	1261.0
87	957.5
88	654.0
89	654.0
90	496.5
91	339.0
92	228.5
93	118.0
94	87.0
95	56.0
96	56.0
97	38.0
98	20.0
99	15.5
100	11.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	3355189.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	68.66673045966321
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.3955693241047	57.26501080308455
2	9.437419392400633	12.960734673055457
3	2.9993877019675796	6.178744406251093
4	1.399162045488096	3.8430353218768842
5	0.7525849733630882	2.5838774756958003
6	0.47903873177898104	1.973641408488371
7	0.31329154409972537	1.5058894209791274
8	0.23239709619928828	1.276635901145996
9	0.16964629633301911	1.0484150853401617
>10	0.7950186654688557	9.022668685083197
>50	0.01979005240549167	0.9060332152730225
>100	0.006389827906910432	0.7466445271579759
>500	1.3043506657266354E-4	0.057471192657279103
>1k	8.695670847704817E-5	0.19611857437383706
>5k	8.695670847704817E-5	0.43507930953720925
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	9521	0.283769409115254	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	5052	0.1505727397174943	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3391	0.10106733182542027	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	5.9609160616585236E-5
2	0.0	2.9804580308292618E-5	0.0	0.0	5.9609160616585236E-5
3	0.0	2.9804580308292618E-5	0.0	0.0	5.9609160616585236E-5
4	0.0	2.9804580308292618E-5	0.0	0.0	5.9609160616585236E-5
5	0.0	2.9804580308292618E-5	0.0	0.0	5.9609160616585236E-5
6	0.0	2.9804580308292618E-5	0.0	0.0	8.941374092487785E-5
7	0.0	2.9804580308292618E-5	0.0	0.0	8.941374092487785E-5
8	0.0	2.9804580308292618E-5	0.0	0.0	1.1921832123317047E-4
9	0.0	2.9804580308292618E-5	0.0	2.9804580308292618E-5	1.4902290154146308E-4
10	0.0	2.9804580308292618E-5	0.0	2.9804580308292618E-5	1.788274818497557E-4
11	5.9609160616585236E-5	2.9804580308292618E-5	0.0	2.9804580308292618E-5	1.788274818497557E-4
12	5.9609160616585236E-5	2.9804580308292618E-5	0.0	2.9804580308292618E-5	2.086320621580483E-4
13	5.9609160616585236E-5	2.9804580308292618E-5	0.0	2.9804580308292618E-5	2.086320621580483E-4
14	5.9609160616585236E-5	2.9804580308292618E-5	0.0	2.9804580308292618E-5	2.3843664246634094E-4
15	5.9609160616585236E-5	2.9804580308292618E-5	0.0	2.9804580308292618E-5	2.6824122277463355E-4
16	1.1921832123317047E-4	2.9804580308292618E-5	0.0	5.9609160616585236E-5	3.278503833912188E-4
17	1.1921832123317047E-4	8.941374092487785E-5	0.0	1.1921832123317047E-4	3.576549636995114E-4
18	1.1921832123317047E-4	8.941374092487785E-5	0.0	1.1921832123317047E-4	3.576549636995114E-4
19	1.1921832123317047E-4	8.941374092487785E-5	0.0	1.788274818497557E-4	3.576549636995114E-4
20	1.1921832123317047E-4	8.941374092487785E-5	0.0	2.3843664246634094E-4	3.576549636995114E-4
21	1.1921832123317047E-4	8.941374092487785E-5	0.0	3.576549636995114E-4	3.87459544007804E-4
22	1.1921832123317047E-4	8.941374092487785E-5	0.0	5.364824455492671E-4	3.87459544007804E-4
23	1.1921832123317047E-4	8.941374092487785E-5	0.0	6.25896186474145E-4	5.662870258575598E-4
24	1.1921832123317047E-4	8.941374092487785E-5	0.0	0.0011921832123317046	5.662870258575598E-4
25	1.1921832123317047E-4	8.941374092487785E-5	0.0	0.0013412061138731678	5.960916061658523E-4
26	1.1921832123317047E-4	8.941374092487785E-5	0.0	0.0020863206215804833	6.25896186474145E-4
27	1.1921832123317047E-4	8.941374092487785E-5	0.0	0.006914662631523887	6.557007667824376E-4
28	1.1921832123317047E-4	8.941374092487785E-5	0.0	0.030341062753841883	6.557007667824376E-4
29	1.1921832123317047E-4	8.941374092487785E-5	0.0	0.07215688892637642	6.557007667824376E-4
30	1.1921832123317047E-4	8.941374092487785E-5	0.0	0.13274960069313532	6.557007667824376E-4
31	1.1921832123317047E-4	8.941374092487785E-5	0.0	0.39672876848368305	6.557007667824376E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTATAC	1640	0.0	18.951218	37
TCTTATA	3050	0.0	15.891804	37
GGTATCA	2595	0.0	15.256262	1
AACGCAG	4745	0.0	13.879873	5
GTATTAG	320	0.0	13.875001	1
GCGCTTA	80	0.0063019437	13.875001	13
TCGCGTA	95	0.0012459458	13.6315775	23
CTCTTAT	4935	0.0	13.457954	37
CTAATAC	320	0.0	13.296876	3
TATACTG	565	0.0	12.442477	5
TCAACGC	5220	0.0	12.439655	3
CAACGCA	5215	0.0	12.416108	4
TATCAAC	5240	0.0	12.392176	1
ATCAACG	5210	0.0	12.357005	2
GTATAAG	435	0.0	12.333333	1
ACGCAGA	5555	0.0	11.955895	6
TAGACAG	620	0.0	11.935483	5
CGCGAAT	140	1.3998397E-4	11.892858	28
TTATACG	140	1.3998397E-4	11.892858	4
ATCGCGT	110	0.0037476155	11.772727	22
>>END_MODULE