FastQCFastQC Report
Thu 9 Feb 2017
SRR2727350.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727350.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4229917
Sequences flagged as poor quality0
Sequence length43
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT45590.10777989260782185No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC19400.021.17010137
TCTTATA35550.016.86075837
GTCTTAG5550.016.6666661
GGTATCA35150.016.2631591
GTATATG2700.015.0740751
GTACCGT2650.014.6603776
CGCTACG6250.014.50410
CGAACGT1702.4419933E-814.1470594
TTACCGT3250.013.66153817
TCGTTAC4650.013.52688123
GTATAAT2501.0913936E-1113.3200011
TGCGTTA3100.013.12903213
GTATCAA43250.013.0890182
AACGCAG65100.013.0437785
ACCGCTA6950.013.0431658
TAAGACT4700.012.9893624
CTCTTAT56750.012.9744537
GTATAGA3600.012.8472221
TCAACGC66650.012.5738943
CAACGCA67200.012.4434524
Produced by FastQC (version 0.11.5)