##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727350.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4229917
Sequences flagged as poor quality	0
Sequence length	43
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.102262526664234	33.0	31.0	34.0	30.0	34.0
2	32.25087158920612	33.0	31.0	34.0	30.0	34.0
3	32.321546262018856	34.0	31.0	34.0	30.0	34.0
4	35.91502173683313	37.0	35.0	37.0	35.0	37.0
5	35.78432413685658	37.0	35.0	37.0	35.0	37.0
6	35.794952714202196	37.0	35.0	37.0	35.0	37.0
7	35.73815372736628	37.0	35.0	37.0	35.0	37.0
8	35.69992153510341	37.0	35.0	37.0	33.0	37.0
9	37.434768578201414	39.0	37.0	39.0	35.0	39.0
10	37.253819874006986	39.0	37.0	39.0	34.0	39.0
11	37.36855333095188	39.0	37.0	39.0	34.0	39.0
12	37.25938972324989	39.0	37.0	39.0	34.0	39.0
13	37.31270660866395	39.0	37.0	39.0	34.0	39.0
14	38.476107214396876	40.0	38.0	41.0	34.0	41.0
15	38.425282103644115	40.0	38.0	41.0	34.0	41.0
16	38.34521977618	40.0	38.0	41.0	34.0	41.0
17	38.28389327733854	40.0	38.0	41.0	33.0	41.0
18	38.22177976541857	40.0	38.0	41.0	33.0	41.0
19	38.20754520715182	40.0	38.0	41.0	34.0	41.0
20	38.07029239580824	40.0	38.0	41.0	33.0	41.0
21	37.97487350224603	40.0	37.0	41.0	33.0	41.0
22	37.89617337645159	40.0	37.0	41.0	33.0	41.0
23	37.76453485966746	40.0	37.0	41.0	32.0	41.0
24	37.62301246100101	40.0	37.0	41.0	32.0	41.0
25	37.52144616549214	39.0	37.0	41.0	32.0	41.0
26	37.11365920418769	39.0	36.0	41.0	31.0	41.0
27	36.87835553274449	39.0	36.0	40.0	30.0	41.0
28	36.689257496069075	39.0	36.0	40.0	30.0	41.0
29	36.63970333224032	39.0	36.0	40.0	30.0	41.0
30	36.546071944201266	39.0	36.0	40.0	30.0	41.0
31	36.314310659050754	39.0	35.0	40.0	29.0	41.0
32	36.19932518770463	39.0	35.0	40.0	29.0	41.0
33	36.00280856574727	39.0	35.0	40.0	27.0	41.0
34	35.82998484367424	38.0	35.0	40.0	27.0	41.0
35	35.65439652834795	38.0	35.0	40.0	26.0	41.0
36	35.48951858866261	38.0	35.0	40.0	25.0	41.0
37	35.23731434919409	38.0	34.0	40.0	25.0	41.0
38	35.121923432540164	38.0	34.0	40.0	24.0	41.0
39	34.930931505275396	38.0	34.0	40.0	24.0	41.0
40	34.798290604756545	38.0	33.0	40.0	24.0	41.0
41	34.65036547998459	38.0	33.0	40.0	23.0	41.0
42	34.45359022411078	38.0	33.0	40.0	23.0	41.0
43	30.785399571670084	35.0	27.0	38.0	11.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	1.0
10	5.0
11	5.0
12	20.0
13	16.0
14	41.0
15	74.0
16	147.0
17	300.0
18	647.0
19	1326.0
20	2617.0
21	4961.0
22	8634.0
23	13792.0
24	20965.0
25	29012.0
26	39177.0
27	50279.0
28	63744.0
29	78873.0
30	97268.0
31	119561.0
32	146213.0
33	180028.0
34	228138.0
35	296359.0
36	405729.0
37	617101.0
38	1098545.0
39	726338.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.19046922197292	17.133551320274133	11.71410691982845	27.961872537924503
2	20.12916565502349	19.841736847318753	32.762037647547224	27.267059850110538
3	19.071508968142876	20.418249341535542	29.775052323721717	30.73518936659986
4	15.619786392971777	14.810597938446545	31.646412920158955	37.923202748422725
5	15.423092226159522	35.934038422030504	33.3503707046734	15.292498647136574
6	38.64238943695585	33.3861160869114	13.238912252888177	14.73258222324457
7	32.05876143669013	29.953495541401875	18.59301258157075	19.394730440337245
8	29.39821277817035	33.568223679093464	17.69171830085555	19.34184524188063
9	28.165328066720928	12.68989438799863	16.47086692244789	42.67391062283255
10	16.382425470759827	26.450779057839668	32.43474990171202	24.73204556968848
11	39.519475204832624	19.576601621261126	19.63854137090633	21.265381802999915
12	23.229391971520954	23.539398054382627	28.155209664870494	25.076000309225925
13	33.498718769186254	18.202390259667034	21.94728643611683	26.35160453502988
14	23.75346844867169	18.770912053357076	23.441145535479773	34.03447396249146
15	27.429001561969184	26.21370584812894	19.921289235698953	26.43600335420293
16	26.88896732489077	24.614525533243324	22.010053625165696	26.48645351670021
17	26.542601190519814	24.51161571255417	22.815152164924278	26.130630932001743
18	26.478793791934923	23.91415718086194	23.26440447885857	26.34264454834457
19	27.727754468941118	23.847725617311166	23.15095071605424	25.27356919769348
20	27.00849685703053	23.4833449450663	22.996810575715788	26.511347622187387
21	26.72690740740303	23.71909425173118	23.388733159539537	26.16526518132625
22	27.487182372609205	23.360269244053725	22.41661006587127	26.735938317465802
23	26.812653770747747	23.297691184011413	22.90158412091774	26.9880709243231
24	26.62116065161562	23.34270388757037	22.95376008559979	27.08237537521422
25	26.961380093273696	22.777799186130604	22.986030222342425	27.274790498253275
26	26.542577549393997	23.14494587009627	23.284002972162337	27.028473608347397
27	27.115094693347412	23.523534858958225	22.880685365693935	26.480685082000427
28	26.669837729676495	23.621172708589793	22.76656965136668	26.94241991036704
29	26.783977085129568	23.82089293950685	22.994801080021194	26.40032889534239
30	27.1129433508979	23.57883145224835	22.761415885938188	26.54680931091556
31	26.883411660323357	22.822102655914996	23.150005071021486	27.144480612740157
32	26.31479530213004	23.44393518832639	23.026669317624908	27.21460019191866
33	26.43425391089234	22.95489485963909	23.287974681299893	27.322876548168672
34	26.82421428127313	22.89456270654956	23.72278226735891	26.558440744818395
35	26.55955187773188	22.97446971181704	23.58743682204639	26.87854158840469
36	26.29869569544745	22.56413069098046	24.013142574665178	27.12403103890691
37	26.065783323880826	22.30431471823206	24.29423556065048	27.335666397236636
38	25.893344952158635	22.042607455418157	24.62010483893656	27.44394275348665
39	25.503290017274573	22.022701627478742	25.24609820949205	27.227910145754635
40	25.895827270369608	21.554749183021794	25.6120864782926	26.937337068315998
41	25.251157410417274	21.57113248321421	26.066705327787755	27.11100477858076
42	24.720886958302017	21.540328096272336	26.29354193001896	27.445243015406685
43	24.85055853341803	20.445743970862786	26.177203949864737	28.526493545854446
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	48.0
1	59.5
2	71.0
3	197.0
4	323.0
5	323.0
6	393.0
7	463.0
8	467.5
9	472.0
10	680.0
11	888.0
12	888.0
13	1774.5
14	2661.0
15	4668.5
16	6676.0
17	5126.5
18	3577.0
19	3577.0
20	4402.0
21	5227.0
22	5264.0
23	5301.0
24	7136.0
25	8971.0
26	8971.0
27	11829.5
28	14688.0
29	18675.0
30	22662.0
31	29385.5
32	36109.0
33	36109.0
34	45663.0
35	55217.0
36	69269.5
37	83322.0
38	103264.0
39	123206.0
40	123206.0
41	151159.0
42	179112.0
43	208868.5
44	238625.0
45	273069.0
46	307513.0
47	307513.0
48	343260.0
49	379007.0
50	404605.0
51	430203.0
52	437780.5
53	445358.0
54	445358.0
55	434744.5
56	424131.0
57	402682.5
58	381234.0
59	351286.0
60	321338.0
61	321338.0
62	283483.5
63	245629.0
64	211061.0
65	176493.0
66	146393.0
67	116293.0
68	116293.0
69	95279.0
70	74265.0
71	60789.0
72	47313.0
73	39726.5
74	32140.0
75	32140.0
76	26840.5
77	21541.0
78	18154.0
79	14767.0
80	12132.0
81	9497.0
82	9497.0
83	7832.5
84	6168.0
85	5010.0
86	3852.0
87	3427.0
88	3002.0
89	3002.0
90	2248.0
91	1494.0
92	1075.5
93	657.0
94	469.5
95	282.0
96	282.0
97	180.5
98	79.0
99	60.5
100	42.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	4229917.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.511632301594304
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.18261742877789	48.08639092575247
2	9.281763500152366	10.861822660625483
3	3.085678830748699	5.416443154367439
4	1.5194745783302466	3.556277512755178
5	0.8800857286214027	2.5747626273488113
6	0.5727788655059933	2.0108535821166615
7	0.4067195996761917	1.6658479366273478
8	0.310447565355556	1.453183503440757
9	0.23085371024484216	1.21568646683745
>10	1.4012676222217226	15.102529956111157
>50	0.09223530611693874	3.6718389706190417
>100	0.03385047387328899	3.297007305413626
>500	0.0019433807459872943	0.775386303239996
>1k	2.834096287983377E-4	0.31196909474458717
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	4559	0.10777989260782185	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	4.7282251637561685E-5
2	0.0	0.0	0.0	0.0	4.7282251637561685E-5
3	0.0	0.0	0.0	0.0	4.7282251637561685E-5
4	0.0	0.0	0.0	0.0	4.7282251637561685E-5
5	2.3641125818780842E-5	0.0	0.0	0.0	4.7282251637561685E-5
6	9.456450327512337E-5	0.0	0.0	0.0	4.7282251637561685E-5
7	9.456450327512337E-5	0.0	0.0	0.0	7.092337745634252E-5
8	1.182056290939042E-4	0.0	0.0	0.0	7.092337745634252E-5
9	1.182056290939042E-4	0.0	0.0	0.0	1.654878807314659E-4
10	1.4184675491268504E-4	2.3641125818780842E-5	0.0	0.0	1.654878807314659E-4
11	1.4184675491268504E-4	2.3641125818780842E-5	0.0	0.0	1.654878807314659E-4
12	1.4184675491268504E-4	2.3641125818780842E-5	0.0	0.0	1.8912900655024674E-4
13	1.4184675491268504E-4	2.3641125818780842E-5	0.0	0.0	1.8912900655024674E-4
14	1.654878807314659E-4	2.3641125818780842E-5	0.0	0.0	2.1277013236902756E-4
15	1.654878807314659E-4	2.3641125818780842E-5	0.0	0.0	2.6005238400658926E-4
16	1.654878807314659E-4	2.3641125818780842E-5	0.0	9.456450327512337E-5	3.546168872817126E-4
17	1.654878807314659E-4	2.3641125818780842E-5	0.0	2.836935098253701E-4	3.546168872817126E-4
18	1.654878807314659E-4	2.3641125818780842E-5	0.0	3.0733463564415096E-4	3.546168872817126E-4
19	1.654878807314659E-4	2.3641125818780842E-5	0.0	4.49181390556836E-4	3.546168872817126E-4
20	1.8912900655024674E-4	2.3641125818780842E-5	0.0	6.855926487446444E-4	3.546168872817126E-4
21	1.8912900655024674E-4	2.3641125818780842E-5	0.0	0.0010638506618451378	3.546168872817126E-4
22	1.8912900655024674E-4	2.3641125818780842E-5	0.0	0.0017021610589522205	3.546168872817126E-4
23	1.8912900655024674E-4	2.3641125818780842E-5	0.0	0.0021749835753278376	5.201047680131785E-4
24	1.8912900655024674E-4	2.3641125818780842E-5	0.0	0.002955140727347605	5.201047680131785E-4
25	1.8912900655024674E-4	2.3641125818780842E-5	0.0	0.003286116488810537	5.673870196507402E-4
26	1.8912900655024674E-4	2.3641125818780842E-5	0.0	0.004018991389192743	5.673870196507402E-4
27	1.8912900655024674E-4	2.3641125818780842E-5	0.0	0.007115978871453033	5.673870196507402E-4
28	1.8912900655024674E-4	2.3641125818780842E-5	0.0	0.02676175442685991	5.673870196507402E-4
29	1.8912900655024674E-4	2.3641125818780842E-5	0.0	0.07472959871316624	5.673870196507402E-4
30	1.8912900655024674E-4	2.3641125818780842E-5	0.0	0.14135029127049065	5.673870196507402E-4
31	1.8912900655024674E-4	2.3641125818780842E-5	0.0	0.40173365103854286	5.673870196507402E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTATAC	1940	0.0	21.170101	37
TCTTATA	3555	0.0	16.860758	37
GTCTTAG	555	0.0	16.666666	1
GGTATCA	3515	0.0	16.263159	1
GTATATG	270	0.0	15.074075	1
GTACCGT	265	0.0	14.660377	6
CGCTACG	625	0.0	14.504	10
CGAACGT	170	2.4419933E-8	14.147059	4
TTACCGT	325	0.0	13.661538	17
TCGTTAC	465	0.0	13.526881	23
GTATAAT	250	1.0913936E-11	13.320001	1
TGCGTTA	310	0.0	13.129032	13
GTATCAA	4325	0.0	13.089018	2
AACGCAG	6510	0.0	13.043778	5
ACCGCTA	695	0.0	13.043165	8
TAAGACT	470	0.0	12.989362	4
CTCTTAT	5675	0.0	12.97445	37
GTATAGA	360	0.0	12.847222	1
TCAACGC	6665	0.0	12.573894	3
CAACGCA	6720	0.0	12.443452	4
>>END_MODULE