FastQCFastQC Report
Thu 9 Feb 2017
SRR2727349.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727349.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4268163
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT230520.5400918381045897No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT83460.1955407982309954No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77010.18042891051724125No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT61460.14399637502129137No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC23300.019.69098937
AACGCAG90300.016.24645
TCTTATA42100.015.68764937
TAGATCG1406.005357E-714.5357145
TCGTTAC4100.014.43902523
ACGCAGA105150.014.0575376
CGCAGAG106600.013.8663237
CTCGTTA4150.013.81927722
TTATACT7150.013.7132864
CTCTTAT66700.013.61844137
TTAGTAC2051.4279067E-913.5365863
AGAGTAC109450.013.50525410
GAGTACT69250.013.4108311
CAGAGTA113050.013.3533829
GGACCGT2501.0913936E-1113.326
GCAGAGT116300.012.9006888
AGTACTT72400.012.852900512
ATCAACG115100.012.8262382
TAGACAG8800.012.8238645
CAACGCA115500.012.7818174
Produced by FastQC (version 0.11.5)