##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727349.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4268163
Sequences flagged as poor quality	0
Sequence length	43
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.10548472492733	33.0	31.0	34.0	30.0	34.0
2	32.255609731868255	33.0	31.0	34.0	30.0	34.0
3	32.335781927728625	34.0	31.0	34.0	30.0	34.0
4	35.91681878128834	37.0	35.0	37.0	35.0	37.0
5	35.79507413376668	37.0	35.0	37.0	35.0	37.0
6	35.81002459371866	37.0	35.0	37.0	35.0	37.0
7	35.75776089151234	37.0	35.0	37.0	35.0	37.0
8	35.73183849820168	37.0	35.0	37.0	33.0	37.0
9	37.4525314520556	39.0	37.0	39.0	35.0	39.0
10	37.29274022571303	39.0	37.0	39.0	34.0	39.0
11	37.39562242585394	39.0	37.0	39.0	34.0	39.0
12	37.30026922589414	39.0	37.0	39.0	34.0	39.0
13	37.362292630342374	39.0	37.0	39.0	34.0	39.0
14	38.526571501603854	40.0	38.0	41.0	34.0	41.0
15	38.49382837534555	40.0	38.0	41.0	34.0	41.0
16	38.430699108726635	40.0	38.0	41.0	34.0	41.0
17	38.390638080129555	40.0	38.0	41.0	34.0	41.0
18	38.34452339331933	40.0	38.0	41.0	34.0	41.0
19	38.347454162364464	40.0	38.0	41.0	34.0	41.0
20	38.23305693807851	40.0	38.0	41.0	34.0	41.0
21	38.15630518328377	40.0	38.0	41.0	33.0	41.0
22	38.081774993129365	40.0	38.0	41.0	33.0	41.0
23	37.97243169953912	40.0	37.0	41.0	33.0	41.0
24	37.86293400697209	40.0	37.0	41.0	33.0	41.0
25	37.77924343564199	40.0	37.0	41.0	33.0	41.0
26	37.41850978980887	39.0	37.0	41.0	32.0	41.0
27	37.206684936821766	39.0	37.0	41.0	31.0	41.0
28	37.05763111671227	39.0	36.0	40.0	31.0	41.0
29	37.01593284979978	39.0	36.0	40.0	31.0	41.0
30	36.93322747983149	39.0	36.0	40.0	30.0	41.0
31	36.7266210311087	39.0	36.0	40.0	30.0	41.0
32	36.62076143764894	39.0	36.0	40.0	30.0	41.0
33	36.44191798673106	39.0	35.0	40.0	30.0	41.0
34	36.306904164625394	39.0	35.0	40.0	30.0	41.0
35	36.15098743885836	39.0	35.0	40.0	29.0	41.0
36	36.00102081387239	38.0	35.0	40.0	28.0	41.0
37	35.75876905357176	38.0	35.0	40.0	27.0	41.0
38	35.65655903956807	38.0	35.0	40.0	27.0	41.0
39	35.487097610845694	38.0	35.0	40.0	26.0	41.0
40	35.348115336738545	38.0	34.0	40.0	25.0	41.0
41	35.21493227882815	38.0	34.0	40.0	25.0	41.0
42	35.008602998526534	38.0	34.0	40.0	24.0	41.0
43	31.603643534700993	35.0	29.0	38.0	15.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	3.0
10	8.0
11	10.0
12	14.0
13	18.0
14	31.0
15	42.0
16	97.0
17	184.0
18	366.0
19	797.0
20	1793.0
21	3444.0
22	6225.0
23	10384.0
24	16054.0
25	22659.0
26	32018.0
27	42227.0
28	54993.0
29	70022.0
30	88150.0
31	110386.0
32	137243.0
33	171832.0
34	221063.0
35	289645.0
36	404947.0
37	636011.0
38	1143622.0
39	803875.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.14175981563966	18.872568831134142	12.750239388701884	26.235431964524313
2	18.738108174406648	21.303731839669666	34.34341659397732	25.61474339194637
3	18.684384827852167	22.095665043720214	30.744912975441657	28.475037152985955
4	14.910958180369398	15.76444011158899	33.29762710561898	36.026974602422634
5	14.618724730053657	37.032676587093796	33.87356574713758	14.475032935714967
6	36.32499508570783	35.22046838417371	14.068652017273006	14.385884512845456
7	30.21192021017004	30.867096687731937	20.0045312233858	18.916451878712223
8	28.082643516660443	35.007941355566786	18.61313637740639	18.296278750366376
9	26.923948312189577	13.7094576753512	18.238056981422687	41.12853703103654
10	15.732388852065865	27.83077872143121	33.561745416002154	22.87508701050077
11	37.323223129013584	21.108237900005225	20.969841123687168	20.598697847294023
12	21.647298849645622	25.1480086397825	29.92287314237999	23.28181936819189
13	31.871838071788726	19.629592403101757	23.54181412471829	24.956755400391224
14	22.456171425505538	20.136695810352133	25.584753909351633	31.82237885479069
15	25.537051888599382	28.170878197482146	21.48184125114247	24.810228662775998
16	25.009283853498566	26.168681936467745	23.71146556492805	25.110568645105637
17	24.503445627545155	26.326970174288096	25.1005643411463	24.069019857020457
18	24.516847177579677	25.58283270812291	25.465217706071673	24.435102408225738
19	25.376491010301155	25.483398829894732	25.65469031993389	23.485419839870218
20	25.071254307766594	25.30458653992362	25.359106482109517	24.26505267020027
21	24.806479977451655	25.394414412008164	25.4391643430675	24.35994126747268
22	25.283945341356457	25.10014261404731	24.980700127900455	24.63521191669578
23	24.81531281724714	25.150351568110214	25.113099007699564	24.921236606943083
24	24.771031471853348	25.070012555752907	25.2046840760299	24.954271896363846
25	24.976342281210908	24.86451431212913	25.242522368522476	24.916621038137485
26	24.58083255020954	25.176943804629765	25.51926906259203	24.72295458256866
27	24.86692752830667	25.46479597897269	25.16021529636989	24.50806119635075
28	24.724805495947557	25.48311767849541	25.214618092139407	24.577458733417632
29	24.65360390406833	25.728375415840492	25.317332070026378	24.3006886100648
30	24.791438377587735	25.547243626824933	25.150679578076097	24.51063841751123
31	24.724219763865626	24.975264534180162	25.39326637712758	24.90724932482663
32	24.298345681737086	25.372062875761774	25.384902122997644	24.944689319503496
33	24.464225007339223	24.99614002558009	25.41025260750351	25.129382359577175
34	24.67832179792571	24.771710921068387	25.78577715986948	24.764190121136423
35	24.495807681196805	24.865428054176938	25.720901474475085	24.917862790151172
36	24.36329165498131	24.560472503041705	26.063133015304242	25.01310282667274
37	24.065997479477705	24.207861789720777	26.377741431149655	25.348399299651863
38	23.706193976190693	24.265497826582536	26.767370412048464	25.260937785178307
39	23.5175179579599	24.010634083093827	27.336748854249475	25.1350991046968
40	23.701180109569385	23.8790786574927	27.574064064563608	24.845677168374312
41	23.247659473173822	23.837397962542667	27.861096213991825	25.053846350291682
42	22.912831585860243	24.078368141048035	27.80737286743735	25.201427405654375
43	23.08065554197438	23.222098125118464	27.740693127230614	25.956553205676542
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	468.0
1	514.5
2	561.0
3	1283.0
4	2005.0
5	2005.0
6	2348.0
7	2691.0
8	2772.5
9	2854.0
10	4395.5
11	5937.0
12	5937.0
13	11259.5
14	16582.0
15	26380.0
16	36178.0
17	27416.0
18	18654.0
19	18654.0
20	22237.0
21	25820.0
22	23912.5
23	22005.0
24	27119.5
25	32234.0
26	32234.0
27	39800.0
28	47366.0
29	55330.5
30	63295.0
31	74849.0
32	86403.0
33	86403.0
34	100962.0
35	115521.0
36	133212.0
37	150903.0
38	172182.5
39	193462.0
40	193462.0
41	215602.5
42	237743.0
43	260853.0
44	283963.0
45	305850.0
46	327737.0
47	327737.0
48	348677.0
49	369617.0
50	377570.0
51	385523.0
52	382797.0
53	380071.0
54	380071.0
55	364925.5
56	349780.0
57	326556.0
58	303332.0
59	276472.5
60	249613.0
61	249613.0
62	218534.0
63	187455.0
64	159914.5
65	132374.0
66	109852.5
67	87331.0
68	87331.0
69	71616.0
70	55901.0
71	46100.0
72	36299.0
73	29474.0
74	22649.0
75	22649.0
76	18520.5
77	14392.0
78	11719.5
79	9047.0
80	7213.0
81	5379.0
82	5379.0
83	4247.5
84	3116.0
85	2448.0
86	1780.0
87	1469.5
88	1159.0
89	1159.0
90	856.5
91	554.0
92	407.0
93	260.0
94	187.0
95	114.0
96	114.0
97	68.0
98	22.0
99	17.5
100	13.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	4268163.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	61.08296540971446
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.41640462469013	50.34238392882979
2	9.340897488093102	11.411394363217594
3	3.035409978472683	5.562355281580471
4	1.466568843740509	3.583294958126658
5	0.8742314918356975	2.67003259879415
6	0.5735007712206416	2.1018676662549014
7	0.3918304267122753	1.6753915080937698
8	0.29088069193175276	1.421428419489685
9	0.2168728237403593	1.192251167175552
>10	1.3080630263800765	14.43336439383648
>50	0.06928084993000656	2.835399120979981
>100	0.015444932810211491	1.4596033500449386
>500	3.4540345063497664E-4	0.15019619813252366
>1k	1.1513442512864778E-4	0.10038081508248116
>5k	1.151344251286278E-4	0.5202595584131733
>10k+	3.83781417095426E-5	0.5403966719479326
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	23052	0.5400918381045897	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	8346	0.1955407982309954	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7701	0.18042891051724125	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	6146	0.14399637502129137	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.34292832771382E-5
2	0.0	0.0	0.0	0.0	2.34292832771382E-5
3	0.0	0.0	0.0	0.0	2.34292832771382E-5
4	0.0	0.0	0.0	0.0	2.34292832771382E-5
5	0.0	0.0	0.0	0.0	2.34292832771382E-5
6	0.0	0.0	0.0	0.0	4.68585665542764E-5
7	0.0	0.0	0.0	0.0	4.68585665542764E-5
8	0.0	0.0	0.0	2.34292832771382E-5	4.68585665542764E-5
9	0.0	0.0	0.0	2.34292832771382E-5	7.028784983141459E-5
10	2.34292832771382E-5	0.0	0.0	4.68585665542764E-5	7.028784983141459E-5
11	2.34292832771382E-5	0.0	0.0	4.68585665542764E-5	7.028784983141459E-5
12	2.34292832771382E-5	0.0	0.0	7.028784983141459E-5	7.028784983141459E-5
13	2.34292832771382E-5	0.0	0.0	7.028784983141459E-5	7.028784983141459E-5
14	4.68585665542764E-5	0.0	2.34292832771382E-5	7.028784983141459E-5	1.4057569966282918E-4
15	4.68585665542764E-5	0.0	2.34292832771382E-5	1.4057569966282918E-4	1.4057569966282918E-4
16	4.68585665542764E-5	0.0	2.34292832771382E-5	2.577221160485202E-4	2.1086354949424377E-4
17	4.68585665542764E-5	0.0	2.34292832771382E-5	5.154442320970404E-4	2.1086354949424377E-4
18	4.68585665542764E-5	0.0	2.34292832771382E-5	5.623027986513167E-4	2.3429283277138198E-4
19	4.68585665542764E-5	0.0	2.34292832771382E-5	8.434541979769751E-4	2.3429283277138198E-4
20	4.68585665542764E-5	0.0	2.34292832771382E-5	0.0012417520136883244	2.3429283277138198E-4
21	4.68585665542764E-5	0.0	2.34292832771382E-5	0.001991489078556747	2.3429283277138198E-4
22	4.68585665542764E-5	0.0	2.34292832771382E-5	0.0030458068260279655	2.3429283277138198E-4
23	4.68585665542764E-5	0.0	2.34292832771382E-5	0.0035378217748478677	3.5143924915707295E-4
24	4.68585665542764E-5	0.0	2.34292832771382E-5	0.004662427372150502	3.748685324342112E-4
25	4.68585665542764E-5	0.0	2.34292832771382E-5	0.0052013008875246795	3.9829781571134936E-4
26	4.68585665542764E-5	0.0	2.34292832771382E-5	0.006302477201550175	4.2172709898848754E-4
27	4.68585665542764E-5	0.0	2.34292832771382E-5	0.010988333856977815	4.2172709898848754E-4
28	4.68585665542764E-5	0.0	2.34292832771382E-5	0.03685426259493838	4.2172709898848754E-4
29	4.68585665542764E-5	0.0	2.34292832771382E-5	0.09235823467847877	4.2172709898848754E-4
30	7.028784983141459E-5	0.0	2.34292832771382E-5	0.16625419413457265	4.2172709898848754E-4
31	7.028784983141459E-5	0.0	2.34292832771382E-5	0.4786836866352105	4.2172709898848754E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTATAC	2330	0.0	19.690989	37
AACGCAG	9030	0.0	16.2464	5
TCTTATA	4210	0.0	15.687649	37
TAGATCG	140	6.005357E-7	14.535714	5
TCGTTAC	410	0.0	14.439025	23
ACGCAGA	10515	0.0	14.057537	6
CGCAGAG	10660	0.0	13.866323	7
CTCGTTA	415	0.0	13.819277	22
TTATACT	715	0.0	13.713286	4
CTCTTAT	6670	0.0	13.618441	37
TTAGTAC	205	1.4279067E-9	13.536586	3
AGAGTAC	10945	0.0	13.505254	10
GAGTACT	6925	0.0	13.41083	11
CAGAGTA	11305	0.0	13.353382	9
GGACCGT	250	1.0913936E-11	13.32	6
GCAGAGT	11630	0.0	12.900688	8
AGTACTT	7240	0.0	12.8529005	12
ATCAACG	11510	0.0	12.826238	2
TAGACAG	880	0.0	12.823864	5
CAACGCA	11550	0.0	12.781817	4
>>END_MODULE