FastQCFastQC Report
Thu 9 Feb 2017
SRR2727348.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727348.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3120732
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT194080.621905373482888No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT76020.24359669462164646No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62170.19921608135527175No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT59480.1905963088147268No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC23850.020.01257937
AACGCAG83550.015.2561345
TCTTATA41900.015.23269737
TCGTTAC3100.014.32258123
GGTATCA54000.014.1490751
TAGGTCG1051.6570673E-414.0952395
CTCGTTA3550.014.07042322
CGTGATA800.006301813813.87534
GTATTAC2151.9826984E-1013.7674421
GTCTTAG5250.013.7428581
ACGCAGA94600.013.5327696
CGCAGAG94500.013.3708997
GTATCGA1254.8495884E-513.329
GTCTTAT3200.013.2968751
GTGTATA3900.013.282051
GGACCGT2651.8189894E-1213.2641526
TATACCG1551.8902756E-613.1290325
GAGTACT58550.013.11272411
AGAGTAC96750.012.96434210
TATACAC7850.012.96178337
Produced by FastQC (version 0.11.5)