##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727348.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3120732
Sequences flagged as poor quality	0
Sequence length	43
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.13150344214114	33.0	31.0	34.0	30.0	34.0
2	32.275454925318805	34.0	31.0	34.0	30.0	34.0
3	32.35382339784384	34.0	31.0	34.0	30.0	34.0
4	35.92695175362704	37.0	35.0	37.0	35.0	37.0
5	35.811663096991346	37.0	35.0	37.0	35.0	37.0
6	35.82606260326103	37.0	35.0	37.0	35.0	37.0
7	35.77424623453728	37.0	35.0	37.0	35.0	37.0
8	35.75000288393877	37.0	35.0	37.0	35.0	37.0
9	37.477135813007976	39.0	37.0	39.0	35.0	39.0
10	37.3152154686785	39.0	37.0	39.0	34.0	39.0
11	37.42153795968382	39.0	37.0	39.0	35.0	39.0
12	37.32814192311291	39.0	37.0	39.0	34.0	39.0
13	37.390965324802	39.0	37.0	39.0	34.0	39.0
14	38.55242039367687	40.0	38.0	41.0	34.0	41.0
15	38.51929803648631	40.0	38.0	41.0	34.0	41.0
16	38.458920535310305	40.0	38.0	41.0	34.0	41.0
17	38.42719720885997	40.0	38.0	41.0	34.0	41.0
18	38.379858635730336	40.0	38.0	41.0	34.0	41.0
19	38.397298133899355	40.0	38.0	41.0	34.0	41.0
20	38.283121395877636	40.0	38.0	41.0	34.0	41.0
21	38.20929032034792	40.0	38.0	41.0	34.0	41.0
22	38.1364413861876	40.0	38.0	41.0	33.0	41.0
23	38.024904413451715	40.0	37.0	41.0	33.0	41.0
24	37.92103070689825	40.0	37.0	41.0	33.0	41.0
25	37.83563599822093	40.0	37.0	41.0	33.0	41.0
26	37.4857789774963	39.0	37.0	41.0	32.0	41.0
27	37.2796209350883	39.0	37.0	41.0	31.0	41.0
28	37.13230485668106	39.0	36.0	41.0	31.0	41.0
29	37.08600289931977	39.0	36.0	40.0	31.0	41.0
30	37.00776772885336	39.0	36.0	40.0	31.0	41.0
31	36.79416656092224	39.0	36.0	40.0	30.0	41.0
32	36.68451183888908	39.0	36.0	40.0	30.0	41.0
33	36.50557689670244	39.0	35.0	40.0	30.0	41.0
34	36.369098980623775	39.0	35.0	40.0	30.0	41.0
35	36.21621690039388	39.0	35.0	40.0	29.0	41.0
36	36.07196644889724	39.0	35.0	40.0	29.0	41.0
37	35.842756122602005	38.0	35.0	40.0	27.0	41.0
38	35.73413320977258	38.0	35.0	40.0	27.0	41.0
39	35.57008355731924	38.0	35.0	40.0	26.0	41.0
40	35.430799889256754	38.0	34.0	40.0	26.0	41.0
41	35.29519452487429	38.0	34.0	40.0	25.0	41.0
42	35.1015188103304	38.0	34.0	40.0	24.0	41.0
43	31.76103074535077	35.0	29.0	38.0	15.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	2.0
10	16.0
11	14.0
12	9.0
13	10.0
14	14.0
15	28.0
16	64.0
17	125.0
18	277.0
19	577.0
20	1153.0
21	2309.0
22	4212.0
23	6946.0
24	10907.0
25	15892.0
26	21978.0
27	29619.0
28	38316.0
29	49914.0
30	62965.0
31	79087.0
32	98904.0
33	124430.0
34	160746.0
35	211679.0
36	295817.0
37	467710.0
38	831823.0
39	605187.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.904495483751894	19.162747714318307	13.128682629588187	25.80407417234162
2	18.32066963776447	21.684399685714762	34.96503384462363	25.02989683189713
3	18.444679004797592	22.48177671136131	31.234691091705407	27.838853192135694
4	14.621857948712034	15.913862516871042	33.86279244741298	35.601487087003946
5	14.228232350615178	37.57432551080964	34.14106049478135	14.056381643793827
6	35.65602557348724	35.80137608740513	14.227655562861532	14.314942776246086
7	29.6846380913196	31.40971413117179	20.313695632947653	18.591952144560956
8	27.899223643683595	35.61286262325633	18.729740330153312	17.758173402906756
9	27.03362544428679	13.863990884189992	18.403342549119888	40.69904112240334
10	15.555228709161826	28.327104025593997	33.72929812620885	22.38836913903533
11	37.04967296134368	21.4002355857536	21.242836616537403	20.307254836365317
12	21.374953055885605	25.579094904656984	30.20204875010094	22.843903289356472
13	31.71486689661272	19.89997859476559	23.865330313529004	24.519824195092692
14	22.121220277806618	20.449240755053623	26.233973311389764	31.195565655749995
15	25.32008515950745	28.635685473792687	21.907328152497556	24.136901214202307
16	24.41532948039114	26.59818273405086	24.2774772072706	24.7090105782874
17	24.039456127600833	26.735778657058663	25.51080964337854	23.71395557196196
18	24.020037606561537	26.033155041829932	26.009859225335596	23.936948126272938
19	25.095906985925097	25.891233210669807	26.170238264612276	22.84262153879282
20	24.58099574074288	25.702783834049193	25.86713630007319	23.849084125134745
21	24.294364270946687	26.042896346113668	26.032225772671286	23.630513610268363
22	24.887141862870635	25.564354773175012	25.38744115162725	24.16106221232711
23	24.21053778408399	25.678462617103936	25.722202355088484	24.388797243723587
24	24.117482693163016	25.565059735985017	25.81288620746671	24.504571363385256
25	24.45772338028386	25.292110953455794	25.7917052794024	24.458460386857954
26	23.99078164994623	25.62014296645787	26.064750193223894	24.324325190372004
27	24.404434600600116	26.02059388630616	25.667022993323364	23.90794851977036
28	24.187690580286933	26.182735332607866	25.569609950485976	24.05996413661923
29	24.10806182652019	26.32853445922303	25.853325437749863	23.71007827650692
30	24.4480461635283	25.994414131043612	25.54093078162431	24.016608923803776
31	24.1662212583458	25.522729923620485	25.852428212355306	24.458620605678412
32	23.722735563322964	25.975924879162964	25.862489954279955	24.438849603234114
33	23.94576016139803	25.40016252597147	25.88572168324611	24.768355629384388
34	24.116425248948005	25.267212948756896	26.492374225021564	24.123987577273535
35	23.953290445959475	25.251703766936735	26.31023746992693	24.484768317176865
36	23.719851624554753	24.82007426462766	26.796565677539757	24.663508433277833
37	23.411270176356062	24.433498294630876	27.144400736750224	25.01083079226284
38	23.156137726661566	24.296799597017625	27.576254545407934	24.970808130912875
39	22.870243263439473	24.157697617097526	28.280608523897598	24.6914505955654
40	23.077117804412556	24.029810954609367	28.35267494933881	24.54039629163927
41	22.51654419540031	24.055381878354183	28.763123523583566	24.66495040266194
42	22.071232005824275	24.32893949240114	28.68346272605273	24.91636577572185
43	22.06008077592052	23.588279929196098	28.609858199935147	25.741781094948234
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	752.0
1	734.0
2	716.0
3	1395.0
4	2074.0
5	2074.0
6	2310.0
7	2546.0
8	2801.0
9	3056.0
10	4547.0
11	6038.0
12	6038.0
13	10427.5
14	14817.0
15	23333.5
16	31850.0
17	25311.5
18	18773.0
19	18773.0
20	21664.5
21	24556.0
22	22797.0
23	21038.0
24	25356.0
25	29674.0
26	29674.0
27	35693.0
28	41712.0
29	47777.5
30	53843.0
31	62344.0
32	70845.0
33	70845.0
34	81106.0
35	91367.0
36	103897.5
37	116428.0
38	131632.5
39	146837.0
40	146837.0
41	163171.0
42	179505.0
43	195763.5
44	212022.0
45	228089.0
46	244156.0
47	244156.0
48	258081.5
49	272007.0
50	276521.0
51	281035.0
52	275092.5
53	269150.0
54	269150.0
55	254471.0
56	239792.0
57	223029.5
58	206267.0
59	188123.0
60	169979.0
61	169979.0
62	148000.0
63	126021.0
64	106756.0
65	87491.0
66	72277.0
67	57063.0
68	57063.0
69	46708.0
70	36353.0
71	29983.0
72	23613.0
73	19369.5
74	15126.0
75	15126.0
76	12288.0
77	9450.0
78	7820.5
79	6191.0
80	4932.0
81	3673.0
82	3673.0
83	2938.0
84	2203.0
85	1727.0
86	1251.0
87	1022.0
88	793.0
89	793.0
90	587.5
91	382.0
92	279.5
93	177.0
94	125.0
95	73.0
96	73.0
97	49.0
98	25.0
99	18.5
100	12.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	3120732.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	65.66278623086224
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.20547608934876	55.29166175922888
2	8.63845150304352	11.344495888200344
3	2.744216181918509	5.40578641573764
4	1.3033578624891358	3.423284348277506
5	0.7430475538897497	2.4395286345213862
6	0.5094732771566639	2.007206093296491
7	0.3323094746072322	1.5274256195537381
8	0.2568150654486909	1.349055419473783
9	0.19018541846386497	1.123929402913786
>10	1.0284635523674135	11.81603208064052
>50	0.03733426901145945	1.656991748175584
>100	0.010285019042681441	1.1264041757536254
>500	2.923666062067831E-4	0.14897342228076635
>1k	9.745553539276568E-5	0.08578130264256352
>5k	1.461833030891485E-4	0.6324651284354917
>10k+	4.872776769638284E-5	0.6209785608679124
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	19408	0.621905373482888	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	7602	0.24359669462164646	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6217	0.19921608135527175	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	5948	0.1905963088147268	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	3.204376409124526E-5
2	0.0	0.0	0.0	0.0	3.204376409124526E-5
3	0.0	0.0	0.0	0.0	3.204376409124526E-5
4	0.0	0.0	0.0	0.0	3.204376409124526E-5
5	0.0	0.0	0.0	0.0	3.204376409124526E-5
6	3.204376409124526E-5	0.0	0.0	0.0	6.408752818249051E-5
7	3.204376409124526E-5	0.0	0.0	0.0	6.408752818249051E-5
8	3.204376409124526E-5	0.0	0.0	0.0	6.408752818249051E-5
9	3.204376409124526E-5	0.0	0.0	0.0	1.2817505636498103E-4
10	9.613129227373578E-5	0.0	0.0	0.0	1.2817505636498103E-4
11	9.613129227373578E-5	0.0	0.0	0.0	1.2817505636498103E-4
12	9.613129227373578E-5	0.0	0.0	3.204376409124526E-5	1.602188204562263E-4
13	9.613129227373578E-5	0.0	0.0	6.408752818249051E-5	1.602188204562263E-4
14	9.613129227373578E-5	0.0	0.0	1.602188204562263E-4	3.204376409124526E-4
15	9.613129227373578E-5	0.0	0.0	2.2430634863871682E-4	3.204376409124526E-4
16	9.613129227373578E-5	0.0	0.0	5.127002254599241E-4	4.1656893318618834E-4
17	9.613129227373578E-5	0.0	0.0	9.292691586461126E-4	4.1656893318618834E-4
18	9.613129227373578E-5	0.0	0.0	0.001121531743193584	4.4861269727743363E-4
19	1.2817505636498103E-4	0.0	0.0	0.0012817505636498103	4.4861269727743363E-4
20	1.602188204562263E-4	0.0	0.0	0.002146932194113432	4.4861269727743363E-4
21	1.602188204562263E-4	0.0	0.0	0.0033966389936719976	4.4861269727743363E-4
22	1.9226258454747155E-4	0.0	0.0	0.005383352367329204	4.4861269727743363E-4
23	1.9226258454747155E-4	0.0	0.0	0.006408752818249052	5.127002254599241E-4
24	2.2430634863871682E-4	0.0	0.0	0.008235247371450032	5.127002254599241E-4
25	2.2430634863871682E-4	3.204376409124526E-5	0.0	0.009549041699191086	5.447439895511694E-4
26	2.2430634863871682E-4	3.204376409124526E-5	0.0	0.010862836026932143	5.447439895511694E-4
27	2.2430634863871682E-4	3.204376409124526E-5	0.0	0.01893786457792595	5.447439895511694E-4
28	2.2430634863871682E-4	3.204376409124526E-5	0.0	0.06030636401972358	5.447439895511694E-4
29	2.2430634863871682E-4	3.204376409124526E-5	0.0	0.1442289821746949	5.447439895511694E-4
30	2.5635011272996205E-4	3.204376409124526E-5	0.0	0.25442748688448735	5.447439895511694E-4
31	2.5635011272996205E-4	3.204376409124526E-5	0.0	0.706052297986498	5.447439895511694E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTATAC	2385	0.0	20.012579	37
AACGCAG	8355	0.0	15.256134	5
TCTTATA	4190	0.0	15.232697	37
TCGTTAC	310	0.0	14.322581	23
GGTATCA	5400	0.0	14.149075	1
TAGGTCG	105	1.6570673E-4	14.095239	5
CTCGTTA	355	0.0	14.070423	22
CGTGATA	80	0.0063018138	13.875	34
GTATTAC	215	1.9826984E-10	13.767442	1
GTCTTAG	525	0.0	13.742858	1
ACGCAGA	9460	0.0	13.532769	6
CGCAGAG	9450	0.0	13.370899	7
GTATCGA	125	4.8495884E-5	13.32	9
GTCTTAT	320	0.0	13.296875	1
GTGTATA	390	0.0	13.28205	1
GGACCGT	265	1.8189894E-12	13.264152	6
TATACCG	155	1.8902756E-6	13.129032	5
GAGTACT	5855	0.0	13.112724	11
AGAGTAC	9675	0.0	12.964342	10
TATACAC	785	0.0	12.961783	37
>>END_MODULE