FastQCFastQC Report
Thu 9 Feb 2017
SRR2727347.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727347.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3476796
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT183690.5283312567087629No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT71050.2043548140299287No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60570.17421211943409967No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT48530.13958253518469305No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC20800.018.8557737
TACTTAC6550.016.66412231
AACGCAG81200.015.834365
TCTTATA35050.015.20114137
GTACTTA7700.014.65584430
ACGCAGA91550.014.0644466
CGCAGAG92150.013.9327187
CGTATAC800.006302003713.8750013
GGTATCA49700.013.8470821
TCGTTAC3250.013.66153823
AGAGTAC94950.013.44391710
CAACGCA96000.013.3161464
ACGACGG2254.0745363E-1013.15555616
CAGAGTA98400.013.1229699
TCAACGC97100.013.1081363
CGATCTA850.0094097413.05882412
ATCAACG97600.013.0409842
GCAGAGT100200.012.9241518
GAGTACT52650.012.82526111
TTAGACT3900.012.8076934
Produced by FastQC (version 0.11.5)