##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727347.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3476796
Sequences flagged as poor quality	0
Sequence length	43
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.11574507103667	33.0	31.0	34.0	30.0	34.0
2	32.2643721403269	33.0	31.0	34.0	30.0	34.0
3	32.34415910510712	34.0	31.0	34.0	30.0	34.0
4	35.9252593479744	37.0	35.0	37.0	35.0	37.0
5	35.80592965477411	37.0	35.0	37.0	35.0	37.0
6	35.81989998838011	37.0	35.0	37.0	35.0	37.0
7	35.76664693585704	37.0	35.0	37.0	35.0	37.0
8	35.74358518590104	37.0	35.0	37.0	35.0	37.0
9	37.46481070502842	39.0	37.0	39.0	35.0	39.0
10	37.30579102138866	39.0	37.0	39.0	34.0	39.0
11	37.40727583671863	39.0	37.0	39.0	35.0	39.0
12	37.31499691094905	39.0	37.0	39.0	34.0	39.0
13	37.3748013976086	39.0	37.0	39.0	34.0	39.0
14	38.53693141616592	40.0	38.0	41.0	34.0	41.0
15	38.50120168108799	40.0	38.0	41.0	34.0	41.0
16	38.43051217270153	40.0	38.0	41.0	34.0	41.0
17	38.39724188592025	40.0	38.0	41.0	34.0	41.0
18	38.34939582305088	40.0	38.0	41.0	34.0	41.0
19	38.35952842789741	40.0	38.0	41.0	34.0	41.0
20	38.23999394845139	40.0	38.0	41.0	34.0	41.0
21	38.1615835384072	40.0	38.0	41.0	33.0	41.0
22	38.088232959310815	40.0	38.0	41.0	33.0	41.0
23	37.976335971394356	40.0	37.0	41.0	33.0	41.0
24	37.86536512352177	40.0	37.0	41.0	33.0	41.0
25	37.77809655786535	40.0	37.0	41.0	33.0	41.0
26	37.4141925496923	39.0	37.0	41.0	32.0	41.0
27	37.202120573079355	39.0	37.0	41.0	31.0	41.0
28	37.04545650650772	39.0	36.0	40.0	31.0	41.0
29	37.002474404595496	39.0	36.0	40.0	31.0	41.0
30	36.920215048567705	39.0	36.0	40.0	30.0	41.0
31	36.70631293869413	39.0	36.0	40.0	30.0	41.0
32	36.58739713230227	39.0	36.0	40.0	30.0	41.0
33	36.41100426944808	39.0	35.0	40.0	30.0	41.0
34	36.26879747905831	39.0	35.0	40.0	29.0	41.0
35	36.11722660748574	39.0	35.0	40.0	29.0	41.0
36	35.95727848283305	38.0	35.0	40.0	27.0	41.0
37	35.72606647039401	38.0	35.0	40.0	27.0	41.0
38	35.61579224090226	38.0	35.0	40.0	26.0	41.0
39	35.445589847664344	38.0	34.0	40.0	26.0	41.0
40	35.30753630641545	38.0	34.0	40.0	25.0	41.0
41	35.16951267776424	38.0	34.0	40.0	25.0	41.0
42	34.96368351781353	38.0	34.0	40.0	24.0	41.0
43	31.54908369659882	35.0	29.0	38.0	14.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	3.0
10	3.0
11	13.0
12	11.0
13	12.0
14	28.0
15	46.0
16	82.0
17	178.0
18	360.0
19	729.0
20	1596.0
21	3104.0
22	5248.0
23	8640.0
24	13278.0
25	19029.0
26	26099.0
27	34831.0
28	44717.0
29	57654.0
30	71671.0
31	89628.0
32	111540.0
33	139971.0
34	179306.0
35	236654.0
36	329611.0
37	517523.0
38	928397.0
39	656834.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.14667757325998	18.77530346905599	12.781969376402872	26.296049581281157
2	18.89587424743931	21.332686761029407	34.28121753476477	25.490221456766516
3	18.68970742028005	22.09836297556716	30.988933489339036	28.222996114813753
4	14.927105300397262	15.746969336135914	33.31210689381833	36.01381846964849
5	14.506027963676901	37.16044887304288	33.99489069821755	14.338632465062663
6	36.25720347124191	35.25389467774353	14.036515228388435	14.452386622626118
7	29.991463404812933	31.11522792824198	20.01213185933256	18.881176807612526
8	28.248421822850695	34.94711797873675	18.60885712017616	18.1956030782364
9	27.1896596751722	13.489373549670445	18.12732182158516	41.193644953572196
10	15.78093739178255	27.806607002539117	33.46957946339101	22.942876142287325
11	37.43452304938224	21.044231528108064	20.86970302542916	20.65154239708053
12	21.598017254966926	25.16011868398376	29.715519691117915	23.526344369931394
13	32.04985279550483	19.587689355372014	23.37945625800306	24.9830015911201
14	22.43565627664091	20.253014557080714	25.593362394572473	31.717966771705903
15	25.839911228613932	28.181377337065506	21.36691367569452	24.611797758626043
16	24.91719387620096	26.166993979514473	23.80079245374189	25.115019690542674
17	24.565519518545234	26.14024521427199	24.906523132217135	24.387712134965643
18	24.48860962794481	25.561982929110595	25.433531331720356	24.51587611122424
19	25.668143888798767	25.34862557366035	25.519184904722625	23.464045632818262
20	25.023872553926086	25.123245654907567	25.350897780600302	24.50198401056605
21	24.816814101258743	25.367867427367035	25.620197446154446	24.19512102521977
22	25.47641564244782	24.88331210689382	24.868470856501215	24.77180139415715
23	24.72589706154747	25.084589374815202	25.266538502690405	24.922975060946918
24	24.652409862413556	24.902323863695194	25.35854850270191	25.08671777118934
25	25.01593421069283	24.591146561374323	25.237229909376335	25.15568931855651
26	24.508714345046418	24.982685207875296	25.62931503602742	24.879285411050862
27	25.07504035324477	25.33033286968807	25.11036022821011	24.48426654885705
28	24.706827780519767	25.524994851581745	25.00094914973441	24.76722821816408
29	24.653128915242657	25.66066573937614	25.321100231362436	24.36510511401877
30	25.121634976570384	25.34664098785203	24.95101812128178	24.580705914295805
31	24.829757052182526	24.834474038741416	25.476444404560983	24.859324504515076
32	24.380722941466797	25.276001237921353	25.298493210415568	25.044782610196286
33	24.486538755797007	24.743499474803812	25.363725683071426	25.406236086327755
34	24.718073766766874	24.71571527348743	25.863582447747866	24.70262851199783
35	24.604175798637595	24.74942447011559	25.661844986015858	24.984554745230955
36	24.397635064007204	24.345000396917165	26.15105976882164	25.10630477025399
37	24.086889193383794	24.16123925591263	26.45910200080764	25.292769549895937
38	23.95564767101665	24.011589981120547	26.684251822655114	25.34851052520769
39	23.530198493095366	23.983575682898852	27.344313557654807	25.141912266350975
40	23.94227328839541	23.622668686917496	27.498449722100464	24.936608302586635
41	23.430221387737447	23.70938645810683	27.78664609600333	25.07374605815239
42	23.00523240362679	23.889811193984347	27.80312678684628	25.301829615542587
43	23.068566576813826	23.08421316637502	27.595608140368316	26.25161211644284
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	384.0
1	429.5
2	475.0
3	1039.0
4	1603.0
5	1603.0
6	1855.5
7	2108.0
8	2207.0
9	2306.0
10	3385.0
11	4464.0
12	4464.0
13	8528.0
14	12592.0
15	20313.5
16	28035.0
17	21324.5
18	14614.0
19	14614.0
20	17746.0
21	20878.0
22	19350.0
23	17822.0
24	22124.5
25	26427.0
26	26427.0
27	33011.0
28	39595.0
29	45875.0
30	52155.0
31	61504.5
32	70854.0
33	70854.0
34	82238.5
35	93623.0
36	107567.5
37	121512.0
38	138300.5
39	155089.0
40	155089.0
41	173432.0
42	191775.0
43	210497.5
44	229220.0
45	248793.5
46	268367.0
47	268367.0
48	284759.5
49	301152.0
50	310246.5
51	319341.0
52	314673.5
53	310006.0
54	310006.0
55	295937.0
56	281868.0
57	262125.5
58	242383.0
59	222540.0
60	202697.0
61	202697.0
62	177758.5
63	152820.0
64	130981.0
65	109142.0
66	90467.5
67	71793.0
68	71793.0
69	59308.5
70	46824.0
71	38868.0
72	30912.0
73	25411.0
74	19910.0
75	19910.0
76	16440.0
77	12970.0
78	10680.5
79	8391.0
80	6815.0
81	5239.0
82	5239.0
83	4224.0
84	3209.0
85	2520.0
86	1831.0
87	1571.5
88	1312.0
89	1312.0
90	975.5
91	639.0
92	461.0
93	283.0
94	197.0
95	111.0
96	111.0
97	80.5
98	50.0
99	32.5
100	15.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	3476796.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	60.970828973779135
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.51185502961874	50.30816201320142
2	9.215268157557484	11.237250775639
3	3.0715156708667277	5.618185699760931
4	1.445926437026547	3.5263733400244566
5	0.8709709365568622	2.6551910006970334
6	0.5808173166603835	2.1247747967465735
7	0.4018716510088221	1.7151713392148402
8	0.2825120311748126	1.3779994188635565
9	0.22358866818847994	1.2269147803735374
>10	1.309735235089947	14.351876473098004
>50	0.06718225369813302	2.7470398883342955
>100	0.01795422837930897	1.7903959444232491
>500	5.663888312141158E-4	0.2347106251206177
>1k	9.43981370863104E-5	0.17856540114050973
>5k	9.43981370863104E-5	0.37877160512673475
>10k+	4.71990685431552E-5	0.5286168982352979
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	18369	0.5283312567087629	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	7105	0.2043548140299287	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6057	0.17421211943409967	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	4853	0.13958253518469305	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.8762113163959E-5
2	0.0	0.0	0.0	0.0	2.8762113163959E-5
3	0.0	0.0	0.0	0.0	2.8762113163959E-5
4	0.0	0.0	0.0	0.0	5.7524226327918E-5
5	0.0	0.0	0.0	0.0	5.7524226327918E-5
6	0.0	0.0	0.0	0.0	1.15048452655836E-4
7	0.0	0.0	0.0	0.0	1.15048452655836E-4
8	0.0	0.0	0.0	0.0	1.15048452655836E-4
9	0.0	0.0	0.0	0.0	1.15048452655836E-4
10	5.7524226327918E-5	0.0	0.0	2.8762113163959E-5	1.15048452655836E-4
11	8.6286339491877E-5	0.0	0.0	2.8762113163959E-5	1.15048452655836E-4
12	8.6286339491877E-5	0.0	0.0	2.8762113163959E-5	1.15048452655836E-4
13	8.6286339491877E-5	0.0	0.0	2.8762113163959E-5	1.72572678983754E-4
14	8.6286339491877E-5	0.0	0.0	2.8762113163959E-5	2.30096905311672E-4
15	8.6286339491877E-5	5.7524226327918E-5	0.0	2.8762113163959E-5	2.5885901847563103E-4
16	8.6286339491877E-5	5.7524226327918E-5	0.0	2.30096905311672E-4	4.60193810623344E-4
17	8.6286339491877E-5	5.7524226327918E-5	0.0	3.16383244803549E-4	4.60193810623344E-4
18	8.6286339491877E-5	5.7524226327918E-5	0.0	4.60193810623344E-4	4.60193810623344E-4
19	8.6286339491877E-5	5.7524226327918E-5	0.0	6.04004376443139E-4	4.60193810623344E-4
20	1.15048452655836E-4	5.7524226327918E-5	0.0	8.628633949187701E-4	4.889559237873031E-4
21	1.15048452655836E-4	5.7524226327918E-5	0.0	0.001495629884525868	4.889559237873031E-4
22	1.15048452655836E-4	5.7524226327918E-5	0.0	0.0028762113163959	4.889559237873031E-4
23	1.15048452655836E-4	5.7524226327918E-5	0.0	0.0034802156928390392	6.32766489607098E-4
24	1.15048452655836E-4	8.6286339491877E-5	0.0	0.004486889653577604	6.32766489607098E-4
25	1.15048452655836E-4	8.6286339491877E-5	0.0	0.005119656143184702	6.90290715935016E-4
26	1.15048452655836E-4	8.6286339491877E-5	0.0	0.006155092217087227	7.19052829098975E-4
27	1.15048452655836E-4	8.6286339491877E-5	0.0	0.010325598625861281	7.47814942262934E-4
28	1.15048452655836E-4	8.6286339491877E-5	0.0	0.033594148175504115	7.47814942262934E-4
29	1.15048452655836E-4	8.6286339491877E-5	0.0	0.09353439200919467	7.47814942262934E-4
30	1.15048452655836E-4	8.6286339491877E-5	0.0	0.16952389498837436	7.47814942262934E-4
31	1.15048452655836E-4	8.6286339491877E-5	0.0	0.47282037830232204	7.47814942262934E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTATAC	2080	0.0	18.85577	37
TACTTAC	655	0.0	16.664122	31
AACGCAG	8120	0.0	15.83436	5
TCTTATA	3505	0.0	15.201141	37
GTACTTA	770	0.0	14.655844	30
ACGCAGA	9155	0.0	14.064446	6
CGCAGAG	9215	0.0	13.932718	7
CGTATAC	80	0.0063020037	13.875001	3
GGTATCA	4970	0.0	13.847082	1
TCGTTAC	325	0.0	13.661538	23
AGAGTAC	9495	0.0	13.443917	10
CAACGCA	9600	0.0	13.316146	4
ACGACGG	225	4.0745363E-10	13.155556	16
CAGAGTA	9840	0.0	13.122969	9
TCAACGC	9710	0.0	13.108136	3
CGATCTA	85	0.00940974	13.058824	12
ATCAACG	9760	0.0	13.040984	2
GCAGAGT	10020	0.0	12.924151	8
GAGTACT	5265	0.0	12.825261	11
TTAGACT	390	0.0	12.807693	4
>>END_MODULE