FastQCFastQC Report
Thu 9 Feb 2017
SRR2727346.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727346.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2267280
Sequences flagged as poor quality0
Sequence length43
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT116780.5150665114145584No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT38100.1680427648989097No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35100.15481105112734203No Hit
GTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGT30440.1342577890688402No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT28340.12499558942874281No Hit
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTG26190.11551286122578597No Hit
GAGTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTG22750.100340496101055No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC11250.018.41777637
TATCGGC650.001580575417.07692137
TCTTATA18200.015.55219837
TAGATCG855.36701E-415.2352945
CTTACAC3750.014.8000013
ATTATAC2700.014.3888893
GAGTACT36650.014.38608411
AGTACTT37550.014.13981412
CTCTTAT30900.014.12944937
CGTCTAT800.006301114313.8751
GTACTTT38850.013.57142813
TATACCG1254.848444E-513.325
GTATACG850.0094084213.0588241
TTAGGAC4550.013.010993
TACTTTT40850.012.95226414
CTCTTAC3350.012.7014921
TTATACT3150.012.3333334
TATACTG2102.8947397E-812.3333335
TATACAG4050.012.3333335
ATACCCG1501.9453952E-512.3333325
Produced by FastQC (version 0.11.5)