##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727346.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2267280
Sequences flagged as poor quality	0
Sequence length	43
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.664546505063335	31.0	31.0	34.0	30.0	34.0
2	31.838402402879222	31.0	31.0	34.0	30.0	34.0
3	31.904724162873574	33.0	31.0	34.0	30.0	34.0
4	35.59456176563989	37.0	35.0	37.0	33.0	37.0
5	35.353353798383964	37.0	35.0	37.0	33.0	37.0
6	35.3621656786987	37.0	35.0	37.0	33.0	37.0
7	35.30877439045905	37.0	35.0	37.0	33.0	37.0
8	35.26394931371511	37.0	35.0	37.0	32.0	37.0
9	36.91753687237571	39.0	37.0	39.0	33.0	39.0
10	36.68098029356762	38.0	35.0	39.0	32.0	39.0
11	36.80707102783953	38.0	37.0	39.0	33.0	39.0
12	36.70159574468085	38.0	37.0	39.0	32.0	39.0
13	36.77539209978477	38.0	37.0	39.0	33.0	39.0
14	37.781148777389646	39.0	37.0	40.0	33.0	41.0
15	37.720997406584104	39.0	37.0	40.0	33.0	41.0
16	37.625397392470276	39.0	37.0	40.0	32.0	41.0
17	37.56171227197347	39.0	37.0	40.0	32.0	41.0
18	37.514096185737976	39.0	37.0	40.0	32.0	41.0
19	37.514127500793904	39.0	37.0	40.0	32.0	41.0
20	37.38305017465862	39.0	36.0	40.0	32.0	41.0
21	37.292066264422566	39.0	36.0	40.0	32.0	41.0
22	37.205365900991495	39.0	36.0	40.0	31.0	41.0
23	37.072432165414064	39.0	36.0	40.0	31.0	41.0
24	36.94426052362302	39.0	36.0	40.0	31.0	41.0
25	36.84789262905331	39.0	36.0	40.0	31.0	41.0
26	36.43228979217388	38.0	35.0	40.0	30.0	41.0
27	36.1646161920892	38.0	35.0	40.0	29.0	41.0
28	35.99264404925726	38.0	35.0	40.0	29.0	41.0
29	35.91468499700081	38.0	35.0	40.0	28.0	41.0
30	35.82692741963939	38.0	35.0	40.0	28.0	41.0
31	35.59597006104231	38.0	34.0	40.0	27.0	41.0
32	35.47414655446173	38.0	34.0	40.0	27.0	41.0
33	35.27535593310046	38.0	34.0	40.0	26.0	41.0
34	35.09718737870929	38.0	34.0	40.0	25.0	41.0
35	34.920077802476975	38.0	34.0	40.0	25.0	41.0
36	34.74492078614022	38.0	33.0	40.0	24.0	41.0
37	34.46420821424791	38.0	33.0	40.0	24.0	41.0
38	34.34507030450584	38.0	33.0	40.0	24.0	41.0
39	34.1592194170989	38.0	33.0	40.0	23.0	41.0
40	33.99273490702516	37.0	33.0	40.0	23.0	40.0
41	33.830438234360116	37.0	32.0	40.0	22.0	40.0
42	33.58673829434388	37.0	32.0	40.0	21.0	40.0
43	30.280194770826718	34.0	26.0	38.0	11.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	8.0
11	10.0
12	7.0
13	22.0
14	32.0
15	40.0
16	117.0
17	235.0
18	486.0
19	970.0
20	1972.0
21	3392.0
22	5816.0
23	9190.0
24	13385.0
25	18531.0
26	24921.0
27	32236.0
28	40959.0
29	51847.0
30	63818.0
31	78060.0
32	95612.0
33	118232.0
34	149951.0
35	196467.0
36	275917.0
37	412971.0
38	524658.0
39	147418.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.759024028792204	19.222989661620975	13.237182879926609	25.78080342966021
2	19.28090046222787	21.84207508556508	33.63329628453477	25.243728167672273
3	19.534155463815672	22.42912211989697	30.36700363431072	27.669718781976645
4	15.644252143537631	15.856047775307857	32.42431459722663	36.07538548392788
5	15.176511061712713	36.652861578631665	33.338758335979676	14.831869023675948
6	36.84066370276278	34.72813238770686	13.541115345259518	14.890088564270846
7	30.175143784622982	30.612231396210436	19.825606012490738	19.38701880667584
8	28.586279594933135	34.603886595391835	18.43861366924244	18.37122014043259
9	27.02612822412759	13.714671324229915	17.794537948555096	41.4646625030874
10	16.092101548992627	27.69653505522035	33.03764863625137	23.173714759535656
11	37.59615045340672	20.877747785893227	20.182553544335065	21.343548216364983
12	22.001825976500477	25.156310645354786	29.22550368723757	23.616359690907167
13	32.92747256624678	19.155904872799127	23.067375886524825	24.849246674429274
14	22.781703186196676	20.086579513778624	25.3992008044882	31.7325164955365
15	26.766654317067147	27.545825835362198	21.10409830281218	24.583421544758476
16	25.504084188984155	25.393511167566423	23.81055714336121	25.29184750008821
17	25.04454676969761	26.135369253025655	24.830193006598215	23.989890970678523
18	25.034975830069513	24.877650753325568	25.31513531632617	24.77223810027875
19	25.78014184397163	25.501923009068133	25.191021841148864	23.52691330581137
20	25.405419709960835	24.95505627888924	24.943059525069685	24.696464486080238
21	24.94574997353657	25.603366147983486	24.776295825835362	24.674588052644577
22	25.59154581701422	25.14285840302036	24.11431318584383	25.151282594121593
23	25.072818531456193	24.758168377968314	24.352351716594335	25.81666137398116
24	25.013805088035003	25.055573197840587	24.498121096644436	25.432500617479974
25	25.411594509720896	24.484404220034577	24.468437952083555	25.63556331816097
26	25.05076567517025	24.64856568222716	25.10845594721428	25.192212695388307
27	25.74115239405808	25.04525246109876	24.67952789245263	24.53406725239053
28	25.31182738788328	25.20645884054903	24.656592921915248	24.825120849652446
29	25.11277830704633	25.218543805793725	25.074450442821355	24.59422744433859
30	24.988179669030732	25.226218199781236	24.957658515930984	24.82794361525705
31	25.16985109911436	24.42331780812251	24.910597720616774	25.49623337214636
32	24.70030168307399	24.863536925302565	24.80372957905508	25.632431812568363
33	25.2661338696588	24.633481528527575	24.61164920080449	25.488735401009137
34	25.430295331851383	24.615751032073675	24.96687660985851	24.987077026216436
35	24.991046540347906	24.819739952718674	25.135977911859143	25.053235595074273
36	24.849511308704702	24.452339366994813	25.31958999329593	25.378559331004553
37	24.28425249638333	24.04511132281853	25.810530679933663	25.86010550086447
38	24.309966126812746	23.986671253660774	26.134443033061643	25.56891958646484
39	24.127721322465685	23.71065770438587	26.48565682227162	25.675964150876823
40	24.387151123813556	23.5623301930066	26.994151582512966	25.056367100666876
41	24.304629335591546	23.250149959422746	27.069395928160617	25.375824776825095
42	23.535425708337744	23.547819413570444	27.31982816414382	25.596926713947994
43	24.00453406725239	22.56271832327723	27.163208778801028	26.269538830669347
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	123.0
1	151.5
2	180.0
3	449.0
4	718.0
5	718.0
6	902.5
7	1087.0
8	1204.0
9	1321.0
10	1824.5
11	2328.0
12	2328.0
13	4800.5
14	7273.0
15	12580.0
16	17887.0
17	13218.5
18	8550.0
19	8550.0
20	10005.5
21	11461.0
22	10316.0
23	9171.0
24	11575.5
25	13980.0
26	13980.0
27	17628.0
28	21276.0
29	24925.0
30	28574.0
31	34055.0
32	39536.0
33	39536.0
34	46400.5
35	53265.0
36	62302.5
37	71340.0
38	82825.0
39	94310.0
40	94310.0
41	106901.5
42	119493.0
43	132397.0
44	145301.0
45	157899.0
46	170497.0
47	170497.0
48	185175.5
49	199854.0
50	208089.0
51	216324.0
52	220309.0
53	224294.0
54	224294.0
55	210846.0
56	197398.0
57	179982.0
58	162566.0
59	148909.0
60	135252.0
61	135252.0
62	118999.5
63	102747.0
64	87913.5
65	73080.0
66	60846.5
67	48613.0
68	48613.0
69	40128.0
70	31643.0
71	26102.5
72	20562.0
73	17083.0
74	13604.0
75	13604.0
76	11309.5
77	9015.0
78	7445.5
79	5876.0
80	4736.5
81	3597.0
82	3597.0
83	2925.0
84	2253.0
85	1764.5
86	1276.0
87	1088.5
88	901.0
89	901.0
90	680.0
91	459.0
92	329.0
93	199.0
94	139.0
95	79.0
96	79.0
97	45.0
98	11.0
99	8.5
100	6.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	2267280.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	64.42297120693982
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.30811020014802	54.31378955935644
2	8.510265295747104	10.965131522226693
3	2.6637446629805184	5.148190371775006
4	1.293717511146285	3.333805038819638
5	0.8022611089792566	2.5842022162109126
6	0.4877876040314125	1.8854836061770706
7	0.35907702533610686	1.6192966205011126
8	0.25729862769587486	1.3260753666909177
9	0.20660800375384678	1.1979271329261645
>10	1.052722754625222	11.89553412442739
>50	0.0413958042019135	1.7751543802195875
>100	0.015093046973206856	1.8070424538464334
>500	0.0010276898470658578	0.42588649049260213
>1k	8.221518776524041E-4	1.2070388491551283
>5k	0.0	0.0
>10k+	6.851265647103367E-5	0.5154422671749586
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	11678	0.5150665114145584	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	3810	0.1680427648989097	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3510	0.15481105112734203	No Hit
GTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGT	3044	0.1342577890688402	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	2834	0.12499558942874281	No Hit
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTG	2619	0.11551286122578597	No Hit
GAGTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTG	2275	0.100340496101055	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	4.410571257189231E-5	0.0	0.0	0.0	0.0
11	8.821142514378462E-5	0.0	0.0	0.0	0.0
12	1.3231713771567693E-4	0.0	0.0	4.410571257189231E-5	0.0
13	1.3231713771567693E-4	0.0	0.0	4.410571257189231E-5	0.0
14	1.7642285028756925E-4	0.0	0.0	1.3231713771567693E-4	1.3231713771567693E-4
15	1.7642285028756925E-4	4.410571257189231E-5	0.0	2.2052856285946157E-4	1.7642285028756925E-4
16	2.2052856285946157E-4	4.410571257189231E-5	0.0	2.6463427543135386E-4	3.087399880032462E-4
17	2.2052856285946157E-4	4.410571257189231E-5	0.0	7.939028262940616E-4	3.087399880032462E-4
18	2.6463427543135386E-4	4.410571257189231E-5	0.0	8.821142514378463E-4	3.528457005751385E-4
19	2.6463427543135386E-4	4.410571257189231E-5	0.0	0.0013231713771567693	3.528457005751385E-4
20	2.6463427543135386E-4	4.410571257189231E-5	0.0	0.0020729684908789387	3.528457005751385E-4
21	2.6463427543135386E-4	4.410571257189231E-5	0.0	0.003131505592604354	3.528457005751385E-4
22	2.6463427543135386E-4	4.410571257189231E-5	0.0	0.00476341695776437	3.969514131470308E-4
23	2.6463427543135386E-4	4.410571257189231E-5	0.0	0.005513214071486539	4.851628382908154E-4
24	2.6463427543135386E-4	4.410571257189231E-5	0.0	0.007101019724074662	4.851628382908154E-4
25	2.6463427543135386E-4	4.410571257189231E-5	0.0	0.007939028262940616	5.292685508627077E-4
26	2.6463427543135386E-4	4.410571257189231E-5	0.0	0.009703256765816308	5.292685508627077E-4
27	2.6463427543135386E-4	4.410571257189231E-5	0.0	0.014422568011008786	5.292685508627077E-4
28	2.6463427543135386E-4	4.410571257189231E-5	0.0	0.03757806711125225	5.292685508627077E-4
29	2.6463427543135386E-4	4.410571257189231E-5	0.0	0.09892911329875445	5.292685508627077E-4
30	2.6463427543135386E-4	4.410571257189231E-5	0.0	0.17712854168871953	5.292685508627077E-4
31	2.6463427543135386E-4	4.410571257189231E-5	0.0	0.44313009420980204	5.292685508627077E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTATAC	1125	0.0	18.417776	37
TATCGGC	65	0.0015805754	17.076921	37
TCTTATA	1820	0.0	15.552198	37
TAGATCG	85	5.36701E-4	15.235294	5
CTTACAC	375	0.0	14.800001	3
ATTATAC	270	0.0	14.388889	3
GAGTACT	3665	0.0	14.386084	11
AGTACTT	3755	0.0	14.139814	12
CTCTTAT	3090	0.0	14.129449	37
CGTCTAT	80	0.0063011143	13.875	1
GTACTTT	3885	0.0	13.571428	13
TATACCG	125	4.848444E-5	13.32	5
GTATACG	85	0.00940842	13.058824	1
TTAGGAC	455	0.0	13.01099	3
TACTTTT	4085	0.0	12.952264	14
CTCTTAC	335	0.0	12.701492	1
TTATACT	315	0.0	12.333333	4
TATACTG	210	2.8947397E-8	12.333333	5
TATACAG	405	0.0	12.333333	5
ATACCCG	150	1.9453952E-5	12.333332	5
>>END_MODULE