FastQCFastQC Report
Thu 9 Feb 2017
SRR2727345.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727345.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5821294
Sequences flagged as poor quality0
Sequence length43
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT291470.5006962369535021No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT105830.18179806757741493No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT92880.1595521545553274No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT81070.13926456901163212No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC31200.019.38942137
TCTTATA54350.015.65777437
AACGCAG133650.015.4062845
GTCTTAG7200.014.6458331
TCTATAC5900.014.1101693
ACGCAGA152650.013.4765816
CGCAGAG153700.013.3965527
AGAGTAC156000.013.16346310
GAGTACT86250.013.08405911
CAGAGTA160200.013.0031229
ATACCCG2850.012.9824565
TAGGGTG8300.012.9277115
CTCTTAT84150.012.92691737
AGTACTT89300.012.65789412
CAACGCA163600.012.6198044
GCAGAGT166650.012.6108628
TCAACGC163800.012.6043973
ATCAACG164100.012.5926282
GGTATCA88350.012.4380311
TATCAAC166600.012.3592431
Produced by FastQC (version 0.11.5)