##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727345.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5821294
Sequences flagged as poor quality	0
Sequence length	43
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.955765333274698	33.0	31.0	34.0	30.0	34.0
2	32.10952461772245	33.0	31.0	34.0	30.0	34.0
3	32.18530209949884	34.0	31.0	34.0	30.0	34.0
4	35.8065194783153	37.0	35.0	37.0	35.0	37.0
5	35.65822753497762	37.0	35.0	37.0	33.0	37.0
6	35.67211018718518	37.0	35.0	37.0	33.0	37.0
7	35.617277876705764	37.0	35.0	37.0	33.0	37.0
8	35.58197163723392	37.0	35.0	37.0	33.0	37.0
9	37.282008089610315	39.0	37.0	39.0	34.0	39.0
10	37.08856381416228	39.0	37.0	39.0	33.0	39.0
11	37.21065282048974	39.0	37.0	39.0	34.0	39.0
12	37.10327909911439	39.0	37.0	39.0	33.0	39.0
13	37.17122258384476	39.0	37.0	39.0	34.0	39.0
14	38.28620183072698	40.0	38.0	41.0	34.0	41.0
15	38.24135407007446	40.0	38.0	41.0	33.0	41.0
16	38.16848728135016	40.0	38.0	41.0	33.0	41.0
17	38.12138246238723	40.0	38.0	41.0	33.0	41.0
18	38.06647800300071	40.0	37.0	41.0	33.0	41.0
19	38.06938457325811	40.0	37.0	41.0	33.0	41.0
20	37.94077605425873	40.0	37.0	41.0	33.0	41.0
21	37.85482282805163	40.0	37.0	41.0	33.0	41.0
22	37.776848068487865	40.0	37.0	41.0	32.0	41.0
23	37.65539122401308	39.0	37.0	41.0	32.0	41.0
24	37.537952730097466	39.0	37.0	41.0	32.0	41.0
25	37.44480265040728	39.0	37.0	41.0	32.0	41.0
26	37.061149119079026	39.0	36.0	40.0	31.0	41.0
27	36.82269543506993	39.0	36.0	40.0	30.0	41.0
28	36.659154648433834	39.0	36.0	40.0	30.0	41.0
29	36.611230767592225	39.0	36.0	40.0	30.0	41.0
30	36.52242748777162	39.0	36.0	40.0	30.0	41.0
31	36.298848503442706	39.0	35.0	40.0	30.0	41.0
32	36.18352672103487	38.0	35.0	40.0	29.0	41.0
33	35.990903397079755	38.0	35.0	40.0	28.0	41.0
34	35.83882123115582	38.0	35.0	40.0	27.0	41.0
35	35.67066360159786	38.0	35.0	40.0	27.0	41.0
36	35.507421889359996	38.0	34.0	40.0	26.0	41.0
37	35.24695110743419	38.0	34.0	40.0	25.0	41.0
38	35.135267691341475	38.0	34.0	40.0	25.0	41.0
39	34.956839664858016	38.0	34.0	40.0	24.0	41.0
40	34.815745949268326	38.0	33.0	40.0	24.0	41.0
41	34.66669128891274	38.0	33.0	40.0	24.0	41.0
42	34.454006274206385	38.0	33.0	40.0	23.0	41.0
43	31.071464866746123	35.0	27.0	38.0	13.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	6.0
10	9.0
11	27.0
12	25.0
13	22.0
14	45.0
15	109.0
16	187.0
17	400.0
18	747.0
19	1687.0
20	3322.0
21	6376.0
22	11005.0
23	17895.0
24	26813.0
25	38180.0
26	51653.0
27	67841.0
28	86900.0
29	109923.0
30	136122.0
31	168474.0
32	207360.0
33	258823.0
34	326169.0
35	427485.0
36	595880.0
37	927792.0
38	1546132.0
39	803884.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.02428188646717	18.920363754175618	12.844343542861775	26.21101081649544
2	19.058666337759266	21.437570409603087	34.01899989933509	25.484763353302547
3	18.75241140543666	22.073940948524502	30.794682419407092	28.378965226631742
4	15.111004529233535	15.751807072448154	33.16979008447263	35.967398313845685
5	14.583303987051677	37.13418356812077	33.860907901232956	14.4216045435946
6	36.39670148939394	35.34308694939647	13.899263634511502	14.360947926698083
7	30.355261218553814	30.965280228073	19.86544572392324	18.814012829449947
8	28.382916238210953	34.82880266827272	18.44931728237742	18.338963811138896
9	27.25928290170536	13.506636153405069	17.87119839678257	41.362882548107
10	15.71030427255521	27.771729103529214	33.35480049624706	23.16316612766852
11	37.725409505171875	20.919077442231917	20.812846765684743	20.54266628691147
12	21.830335317199236	25.06562973799296	29.58812937467168	23.515905570136127
13	32.20491870020652	19.44426788957919	23.314953685555135	25.035859724659154
14	22.592691590563884	19.975782016850548	25.40708302999299	32.02444336259258
15	25.91444445169751	28.013513833865805	21.19951337279993	24.87252834163676
16	25.218671312598197	26.05939504172096	23.46466266778486	25.257270977895978
17	24.79690941567287	26.149959098441002	24.66388057363191	24.38925091225422
18	24.741200152405977	25.35963653442001	25.18172076517695	24.71744254799706
19	25.85122826643011	25.29416999038358	25.231228658095606	23.623373085090705
20	25.296403170841398	25.04362432132787	25.04491269466892	24.615059813161817
21	25.004079848913317	25.27896718495922	25.242927088032317	24.474025878095144
22	25.606471688253507	24.85304813672012	24.642854320706014	24.897625854320363
23	25.020794345724507	24.938956871101166	24.82283148729475	25.217417295879574
24	24.938991227723594	24.95091297570609	24.91425445957548	25.195841336994835
25	25.268505593429914	24.5881070428671	24.950724014282734	25.192663349420247
26	24.86259927775508	24.909427354124357	25.180106003922837	25.047867364197717
27	25.2283942367453	25.287298665897996	24.81685343499229	24.667453662364416
28	24.946429436479242	25.282626165247795	24.8339630329614	24.93698136531156
29	24.90477203178537	25.55208515495008	25.014163517595918	24.528979295668627
30	25.17642984532305	25.386022420444665	24.721307668020202	24.716240066212084
31	25.044174027286715	24.713354109928137	25.06545795488082	25.17701390790433
32	24.600080325783235	25.238477905427896	24.99061205292157	25.1708297158673
33	24.772602105305108	24.754221312306164	25.077087671572677	25.39608891081605
34	25.012497221408157	24.65690617927904	25.521335977877087	24.809260621435715
35	24.824978776196495	24.76758603843063	25.37872163817873	25.028713547194148
36	24.637065229826906	24.398802053289184	25.730842661442626	25.23329005544128
37	24.352403434700257	24.13750276141353	26.047335867248762	25.462757936637452
38	24.125649726675892	24.06434033395324	26.364395957324955	25.445613982045916
39	23.802491336118738	23.979531011489886	26.877872857821643	25.34010479456973
40	24.066693762589555	23.731338771070487	27.162895397483787	25.039072068856168
41	23.635793004098403	23.626946173823207	27.47765359385731	25.259607228221082
42	23.23871634038755	23.798694929340453	27.45573406874829	25.506854661523708
43	23.400261179043696	22.96712724009473	27.325299151700637	26.30731242916094
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	534.0
1	597.5
2	661.0
3	1531.0
4	2401.0
5	2401.0
6	2815.0
7	3229.0
8	3450.5
9	3672.0
10	5367.5
11	7063.0
12	7063.0
13	13510.0
14	19957.0
15	32566.0
16	45175.0
17	34742.0
18	24309.0
19	24309.0
20	28908.0
21	33507.0
22	30831.5
23	28156.0
24	35013.0
25	41870.0
26	41870.0
27	52187.5
28	62505.0
29	72136.0
30	81767.0
31	96945.0
32	112123.0
33	112123.0
34	129743.5
35	147364.0
36	171181.5
37	194999.0
38	222516.5
39	250034.0
40	250034.0
41	282361.5
42	314689.0
43	346828.0
44	378967.0
45	411567.0
46	444167.0
47	444167.0
48	473264.5
49	502362.0
50	518409.5
51	534457.0
52	530953.5
53	527450.0
54	527450.0
55	506529.5
56	485609.0
57	453539.0
58	421469.0
59	386063.5
60	350658.0
61	350658.0
62	308014.5
63	265371.0
64	226059.5
65	186748.0
66	155373.0
67	123998.0
68	123998.0
69	101871.0
70	79744.0
71	66028.0
72	52312.0
73	43159.5
74	34007.0
75	34007.0
76	28297.0
77	22587.0
78	18733.0
79	14879.0
80	12148.0
81	9417.0
82	9417.0
83	7501.0
84	5585.0
85	4445.0
86	3305.0
87	2768.5
88	2232.0
89	2232.0
90	1683.0
91	1134.0
92	816.0
93	498.0
94	362.5
95	227.0
96	227.0
97	145.5
98	64.0
99	48.0
100	32.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	5821294.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.93743595117699
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.49230246609565	45.58470449711167
2	9.530112656318202	10.661801326406012
3	3.240045851538177	5.4371957189788045
4	1.5158580744067995	3.3917285579281935
5	0.9229544655899911	2.581385315239645
6	0.596560989789832	2.0022055294403684
7	0.4188450622020593	1.64003831882761
8	0.31478838333985837	1.40867640249983
9	0.25557087367197634	1.2866381439311267
>10	1.557078626542986	16.434995721588884
>50	0.11332371072599018	4.320031495339465
>100	0.041175673789458855	3.7314085952789537
>500	0.0010757986600012635	0.3920465684731566
>1k	1.8442039750187538E-4	0.14497424530397574
>5k	6.147346581190343E-5	0.299078154218455
>10k+	6.147346581190343E-5	0.6830914094337004
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	29147	0.5006962369535021	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	10583	0.18179806757741493	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9288	0.1595521545553274	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	8107	0.13926456901163212	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.7178311213967205E-5
2	0.0	0.0	0.0	0.0	1.7178311213967205E-5
3	0.0	0.0	0.0	0.0	1.7178311213967205E-5
4	0.0	0.0	0.0	0.0	1.7178311213967205E-5
5	0.0	0.0	0.0	0.0	1.7178311213967205E-5
6	0.0	0.0	0.0	0.0	3.435662242793441E-5
7	0.0	0.0	0.0	0.0	3.435662242793441E-5
8	1.7178311213967205E-5	0.0	0.0	0.0	3.435662242793441E-5
9	3.435662242793441E-5	0.0	0.0	1.7178311213967205E-5	8.589155606983602E-5
10	5.153493364190161E-5	0.0	0.0	5.153493364190161E-5	8.589155606983602E-5
11	5.153493364190161E-5	0.0	0.0	6.871324485586882E-5	1.0306986728380322E-4
12	5.153493364190161E-5	0.0	0.0	6.871324485586882E-5	1.0306986728380322E-4
13	5.153493364190161E-5	0.0	0.0	8.589155606983602E-5	1.0306986728380322E-4
14	5.153493364190161E-5	0.0	0.0	8.589155606983602E-5	1.8896142335363925E-4
15	5.153493364190161E-5	0.0	0.0	1.7178311213967204E-4	2.0613973456760643E-4
16	5.153493364190161E-5	0.0	0.0	3.2638791306537686E-4	2.9203129063744244E-4
17	5.153493364190161E-5	0.0	0.0	6.699541373447209E-4	2.9203129063744244E-4
18	5.153493364190161E-5	0.0	0.0	6.871324485586882E-4	3.2638791306537686E-4
19	6.871324485586882E-5	1.7178311213967205E-5	0.0	9.104504943402618E-4	3.435662242793441E-4
20	8.589155606983602E-5	1.7178311213967205E-5	0.0	0.001271195029833573	3.435662242793441E-4
21	1.0306986728380322E-4	1.7178311213967205E-5	0.0	0.002044219034462097	3.435662242793441E-4
22	1.0306986728380322E-4	1.7178311213967205E-5	0.0	0.0033841273091515392	3.435662242793441E-4
23	1.2024817849777043E-4	1.7178311213967205E-5	0.0	0.0038651200231426207	3.951011579212457E-4
24	1.2024817849777043E-4	1.7178311213967205E-5	0.0	0.005050423496906358	3.951011579212457E-4
25	1.2024817849777043E-4	1.7178311213967205E-5	0.0	0.005634486078181243	4.294577803491801E-4
26	1.2024817849777043E-4	1.7178311213967205E-5	0.0	0.006476223327665636	4.466360915631473E-4
27	1.2024817849777043E-4	1.7178311213967205E-5	0.0	0.010547483085375862	4.466360915631473E-4
28	1.2024817849777043E-4	1.7178311213967205E-5	0.0	0.03385845140272936	4.466360915631473E-4
29	1.3742648971173764E-4	1.7178311213967205E-5	0.0	0.08690507643146009	4.466360915631473E-4
30	1.5460480092570483E-4	1.7178311213967205E-5	0.0	0.15517168519576574	4.466360915631473E-4
31	1.5460480092570483E-4	1.7178311213967205E-5	0.0	0.4479416432154088	4.466360915631473E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTATAC	3120	0.0	19.389421	37
TCTTATA	5435	0.0	15.657774	37
AACGCAG	13365	0.0	15.406284	5
GTCTTAG	720	0.0	14.645833	1
TCTATAC	590	0.0	14.110169	3
ACGCAGA	15265	0.0	13.476581	6
CGCAGAG	15370	0.0	13.396552	7
AGAGTAC	15600	0.0	13.163463	10
GAGTACT	8625	0.0	13.084059	11
CAGAGTA	16020	0.0	13.003122	9
ATACCCG	285	0.0	12.982456	5
TAGGGTG	830	0.0	12.927711	5
CTCTTAT	8415	0.0	12.926917	37
AGTACTT	8930	0.0	12.657894	12
CAACGCA	16360	0.0	12.619804	4
GCAGAGT	16665	0.0	12.610862	8
TCAACGC	16380	0.0	12.604397	3
ATCAACG	16410	0.0	12.592628	2
GGTATCA	8835	0.0	12.438031	1
TATCAAC	16660	0.0	12.359243	1
>>END_MODULE