FastQCFastQC Report
Thu 9 Feb 2017
SRR2727344.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727344.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3147762
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT520131.6523803260856444No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT198490.6305749926455685No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT177240.5630667121593056No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT139350.44269547697697603No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37180.11811566439902381No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCAATC1950.020.87179622
TACTTAC13550.018.84132831
AGTGTAC16800.018.61011927
CTTATAC29750.018.59327737
TAGTACG1800.018.52
GTACTTA14900.017.75503330
GTGTACT16100.017.58074628
GTACGCC2050.017.1463414
ACTTACA12250.016.00816332
TCTATAC2850.015.5789473
CTACAGT21200.015.53301923
AGATCGA2400.015.4166666
TACCGTT1201.9380059E-615.41666618
TGTACTT18500.015.329
CAGTGTA21250.015.06117626
AACGCAG229000.015.034285
CAATCGG3200.015.0312524
CGCTACG9100.014.84065910
AATCGGG3150.014.68253925
ACAGTGT21700.014.66359425
Produced by FastQC (version 0.11.5)