##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727344.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3147762
Sequences flagged as poor quality	0
Sequence length	43
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.99358369533656	33.0	31.0	34.0	30.0	34.0
2	32.13117573692039	33.0	31.0	34.0	30.0	34.0
3	32.18929258311143	34.0	31.0	34.0	30.0	34.0
4	35.78073342266664	37.0	35.0	37.0	35.0	37.0
5	35.67664200787734	37.0	35.0	37.0	35.0	37.0
6	35.7164664927018	37.0	35.0	37.0	35.0	37.0
7	35.65653311781514	37.0	35.0	37.0	33.0	37.0
8	35.65028073914102	37.0	35.0	37.0	33.0	37.0
9	37.347684481863624	39.0	37.0	39.0	34.0	39.0
10	37.17330662229228	39.0	37.0	39.0	34.0	39.0
11	37.30856017704007	39.0	37.0	39.0	34.0	39.0
12	37.202120109461895	39.0	37.0	39.0	34.0	39.0
13	37.276145401081784	39.0	37.0	39.0	34.0	39.0
14	38.29973136469657	40.0	38.0	41.0	34.0	41.0
15	38.29386275074164	40.0	38.0	41.0	34.0	41.0
16	38.189333246922736	40.0	38.0	41.0	33.0	41.0
17	38.216566246113906	40.0	38.0	41.0	33.0	41.0
18	38.18658367436928	40.0	38.0	41.0	33.0	41.0
19	38.23129385258479	40.0	38.0	41.0	34.0	41.0
20	38.10049139674474	40.0	38.0	41.0	33.0	41.0
21	38.017159492998516	40.0	37.0	41.0	33.0	41.0
22	37.93775196472922	40.0	37.0	41.0	33.0	41.0
23	37.80633319799909	40.0	37.0	41.0	33.0	41.0
24	37.682506809599964	40.0	37.0	41.0	32.0	41.0
25	37.58076849520389	40.0	37.0	41.0	32.0	41.0
26	37.18272569527175	39.0	36.0	41.0	31.0	41.0
27	36.92207320629704	39.0	36.0	40.0	31.0	41.0
28	36.69240527079239	39.0	36.0	40.0	30.0	41.0
29	36.61061192046921	39.0	36.0	40.0	30.0	41.0
30	36.489959215468005	39.0	35.0	40.0	30.0	41.0
31	36.19221021157254	39.0	35.0	40.0	30.0	41.0
32	36.04040553256568	38.0	35.0	40.0	29.0	41.0
33	35.81010953178798	38.0	35.0	40.0	28.0	41.0
34	35.59641167280118	38.0	35.0	40.0	27.0	41.0
35	35.40158499912001	38.0	35.0	40.0	26.0	41.0
36	35.18559027016655	38.0	34.0	40.0	25.0	41.0
37	34.923066928185804	38.0	34.0	40.0	24.0	41.0
38	34.75575758268891	38.0	34.0	40.0	24.0	41.0
39	34.504289396720594	38.0	33.0	40.0	23.0	41.0
40	34.320415901837556	38.0	33.0	40.0	22.0	41.0
41	34.082110718662975	38.0	33.0	40.0	21.0	41.0
42	33.812499801446236	38.0	33.0	40.0	19.0	41.0
43	30.687324518181487	35.0	27.0	38.0	10.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	8.0
10	25.0
11	33.0
12	23.0
13	42.0
14	48.0
15	96.0
16	203.0
17	348.0
18	673.0
19	1239.0
20	2307.0
21	4123.0
22	6926.0
23	10459.0
24	15642.0
25	21839.0
26	29718.0
27	38694.0
28	49461.0
29	61173.0
30	75163.0
31	91441.0
32	112783.0
33	140284.0
34	178293.0
35	228563.0
36	320373.0
37	501890.0
38	781041.0
39	474849.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.39272219437174	18.089836525124838	14.174959860370636	25.342481420132778
2	19.28938719000992	23.33016282679567	33.41154763288966	23.968902350304756
3	19.440573969696565	22.509802202326608	32.451976991907266	25.597646836069565
4	15.026199566549186	16.020207372730212	32.09817006495408	36.85542299576652
5	14.682844509845408	38.55818832554685	32.51738854462313	14.24157861998461
6	35.39209762364499	35.017101038769766	13.716062396076959	15.874738941508285
7	28.71020744262114	32.10852662939574	19.675471017186176	19.505794910796943
8	29.84463247221359	34.415848466307175	18.197627393684783	17.541891667794452
9	28.73393858874972	13.113412005100766	17.85763980885467	40.295009597294836
10	16.25176871694874	29.192899590248565	31.420005705641024	23.13532598716167
11	37.22133376030335	21.602109689360248	19.66241412152507	21.51414242881133
12	21.705834176789732	27.12981476998579	28.33298705556519	22.83136399765929
13	33.68415401164383	19.335388126548324	23.18116808068717	23.799289781120684
14	21.97110200834752	21.433863170087193	27.2125719797113	29.38246284185399
15	26.9363439802628	28.537449781781472	21.99842300656784	22.527783231387886
16	23.123603372808997	26.10298999733779	25.439629806827835	25.33377682302537
17	24.49680121940604	25.557237173585552	25.446809511011313	24.499152095997093
18	23.72981184727435	25.262424541626714	27.08857912383465	23.919184487264285
19	26.567002206647132	24.717180015515787	26.83373139392368	21.882086383913396
20	24.125330949417396	24.514845785672488	27.14121969831264	24.218603566597473
21	23.68028459584937	25.69193604853226	28.13595818235305	22.491821173265322
22	24.885077080160446	24.810960930337174	25.91835087913254	24.385611110369844
23	23.374352952986914	25.31973510068423	27.318170814693104	23.987741131635744
24	22.717759474826877	25.094400402571733	27.628867747942827	24.558972374658566
25	23.57481283527789	24.550744306589888	27.0902310911689	24.78421176696332
26	22.989158646682945	25.19485272393529	27.743330023044944	24.07265860633682
27	24.82830658734682	25.799313925258645	26.9366934348912	22.435686052503335
28	23.078301345527393	26.976054733490017	26.08542196011007	23.86022196087252
29	23.24883520418634	26.41085952495773	27.198371414357247	23.14193385649868
30	24.56732751713757	25.811703680265534	26.23644989678381	23.38451890581308
31	23.433855545622574	25.042744654773774	27.478189265897484	24.045210533706168
32	22.851632366106458	26.135298666163454	26.761267211434664	24.251801756295425
33	23.400689124527204	24.745517609018723	26.978024386850088	24.875768879603985
34	23.501014371480437	25.740542010482372	27.98550208052578	22.77294153751141
35	23.639271329916305	25.593453380528768	26.87080535313661	23.89646993641832
36	22.93432603862681	24.827004074640968	28.18643849185548	24.052231394876742
37	22.262547168432683	25.36363930945224	28.174493497284736	24.19932002483034
38	23.34328325966194	24.203354637358224	28.1221070716274	24.331255031352434
39	21.95464587221016	24.77271153282872	29.371725054181354	23.90091754077977
40	23.302079382113387	23.846402618749448	29.186005803488317	23.665512195648844
41	22.039785727129306	24.372014148464846	29.52262591644476	24.065574207961085
42	21.64458431101208	24.717910693375167	29.537334779440123	24.100170216172636
43	21.3855748941629	23.776734073287624	28.8054179445587	26.03227308799077
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1703.0
1	1569.0
2	1435.0
3	2814.5
4	4194.0
5	4194.0
6	4849.5
7	5505.0
8	5790.5
9	6076.0
10	9244.5
11	12413.0
12	12413.0
13	24101.5
14	35790.0
15	57470.0
16	79150.0
17	57718.0
18	36286.0
19	36286.0
20	40651.5
21	45017.0
22	34960.5
23	24904.0
24	29255.5
25	33607.0
26	33607.0
27	39598.0
28	45589.0
29	49518.5
30	53448.0
31	58031.5
32	62615.0
33	62615.0
34	69513.0
35	76411.0
36	86272.0
37	96133.0
38	107486.0
39	118839.0
40	118839.0
41	130784.0
42	142729.0
43	157645.0
44	172561.0
45	195783.5
46	219006.0
47	219006.0
48	242403.0
49	265800.0
50	280512.0
51	295224.0
52	288001.0
53	280778.0
54	280778.0
55	253362.0
56	225946.0
57	212477.5
58	199009.0
59	183868.0
60	168727.0
61	168727.0
62	148383.0
63	128039.0
64	108999.5
65	89960.0
66	75683.0
67	61406.0
68	61406.0
69	52712.5
70	44019.0
71	39140.0
72	34261.0
73	29593.0
74	24925.0
75	24925.0
76	21654.0
77	18383.0
78	15960.0
79	13537.0
80	11007.5
81	8478.0
82	8478.0
83	7222.0
84	5966.0
85	4802.5
86	3639.0
87	3489.0
88	3339.0
89	3339.0
90	2541.5
91	1744.0
92	1235.0
93	726.0
94	525.0
95	324.0
96	324.0
97	203.0
98	82.0
99	60.5
100	39.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	3147762.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.73704681061382
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.38726652611729	47.28583851681055
2	7.54115723341209	8.255613529829532
3	2.089029171970251	3.430418627246204
4	0.9771003394725987	2.1393434808151315
5	0.5726100517076083	1.5671491602273677
6	0.3966211206902997	1.3025921309581832
7	0.28434612529542963	1.0894987019498816
8	0.22098316199440807	0.9676772545956298
9	0.16857041112172982	0.8304341836009088
>10	1.1508567301265002	12.179384410931446
>50	0.12090290481229461	4.563109353594134
>100	0.0824949122970278	8.778575544371247
>500	0.004987573701587969	1.827800589141716
>1k	0.002841757108837191	2.4963137816567533
>5k	0.0	0.0
>10k+	2.3198017214997473E-4	3.2862507342712557
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	52013	1.6523803260856444	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	19849	0.6305749926455685	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	17724	0.5630667121593056	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	13935	0.44269547697697603	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3718	0.11811566439902381	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	3.1768602581770795E-5	0.0	0.0	0.0	0.0
6	3.1768602581770795E-5	0.0	0.0	0.0	0.0
7	3.1768602581770795E-5	0.0	0.0	0.0	0.0
8	3.1768602581770795E-5	0.0	0.0	0.0	0.0
9	3.1768602581770795E-5	0.0	0.0	0.0	0.0
10	3.1768602581770795E-5	0.0	0.0	0.0	0.0
11	1.2707441032708318E-4	0.0	0.0	0.0	0.0
12	1.2707441032708318E-4	0.0	0.0	3.1768602581770795E-5	0.0
13	1.2707441032708318E-4	0.0	0.0	6.353720516354159E-5	0.0
14	1.9061161549062476E-4	0.0	0.0	9.530580774531238E-5	0.0
15	2.2238021807239556E-4	0.0	0.0	2.8591742323593714E-4	0.0
16	2.2238021807239556E-4	0.0	0.0	7.306778593807283E-4	0.0
17	2.2238021807239556E-4	0.0	0.0	0.0010483638851984363	0.0
18	2.5414882065416636E-4	0.0	0.0	0.001238975500689061	0.0
19	3.1768602581770796E-4	0.0	0.0	0.001874347552324477	0.0
20	3.1768602581770796E-4	0.0	0.0	0.0031450916555953085	0.0
21	3.4945462839947874E-4	0.0	0.0	0.0048923647975927026	0.0
22	3.4945462839947874E-4	0.0	0.0	0.00879990291515051	0.0
23	3.4945462839947874E-4	0.0	0.0	0.010610713262311446	3.1768602581770795E-5
24	3.4945462839947874E-4	0.0	0.0	0.014581788585032795	3.1768602581770795E-5
25	3.4945462839947874E-4	0.0	0.0	0.01636083032961196	6.353720516354159E-5
26	3.4945462839947874E-4	0.0	0.0	0.018679938318081228	6.353720516354159E-5
27	3.4945462839947874E-4	0.0	0.0	0.026939774989341633	6.353720516354159E-5
28	3.4945462839947874E-4	0.0	0.0	0.0697320826669869	6.353720516354159E-5
29	3.4945462839947874E-4	0.0	0.0	0.18355898571747165	6.353720516354159E-5
30	3.4945462839947874E-4	0.0	0.0	0.3226419278204642	6.353720516354159E-5
31	3.4945462839947874E-4	0.0	0.0	0.77950620154891	6.353720516354159E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCAATC	195	0.0	20.871796	22
TACTTAC	1355	0.0	18.841328	31
AGTGTAC	1680	0.0	18.610119	27
CTTATAC	2975	0.0	18.593277	37
TAGTACG	180	0.0	18.5	2
GTACTTA	1490	0.0	17.755033	30
GTGTACT	1610	0.0	17.580746	28
GTACGCC	205	0.0	17.146341	4
ACTTACA	1225	0.0	16.008163	32
TCTATAC	285	0.0	15.578947	3
CTACAGT	2120	0.0	15.533019	23
AGATCGA	240	0.0	15.416666	6
TACCGTT	120	1.9380059E-6	15.416666	18
TGTACTT	1850	0.0	15.3	29
CAGTGTA	2125	0.0	15.061176	26
AACGCAG	22900	0.0	15.03428	5
CAATCGG	320	0.0	15.03125	24
CGCTACG	910	0.0	14.840659	10
AATCGGG	315	0.0	14.682539	25
ACAGTGT	2170	0.0	14.663594	25
>>END_MODULE