FastQCFastQC Report
Thu 9 Feb 2017
SRR2727343.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727343.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8728264
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT641320.7347623765733942No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT252860.2897025112897593No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT200190.22935832371706447No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT192560.2206166082969076No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC65150.020.38833437
TAGATCG3200.016.7656255
GGTATCA145850.016.5022281
TACTTAC23700.016.08016831
AACGCAG279100.016.060735
TCTTATA115050.015.54932637
GTACTTA26250.015.29333430
TCGTTAC18100.015.12707123
CGCAGAG311950.014.369457
ACGCAGA316250.014.1682226
AGTGTAC33050.014.04992527
ATCAACG318700.014.018672
TCAACGC321000.013.9643313
CAACGCA321750.013.931784
CGCAATC5450.013.91743122
TATCAAC320950.013.8685161
AGAGTAC322600.013.81478710
CAGAGTA327950.013.747379
TTCGTTA5150.013.65048522
GTGTACT31350.013.63157928
Produced by FastQC (version 0.11.5)