##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727343.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8728264
Sequences flagged as poor quality	0
Sequence length	43
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.071658579529675	33.0	31.0	34.0	30.0	34.0
2	32.221510715074615	33.0	31.0	34.0	30.0	34.0
3	32.298169716223065	34.0	31.0	34.0	30.0	34.0
4	35.88093256574274	37.0	35.0	37.0	35.0	37.0
5	35.766708706336104	37.0	35.0	37.0	35.0	37.0
6	35.78764505748222	37.0	35.0	37.0	35.0	37.0
7	35.73938013332319	37.0	35.0	37.0	35.0	37.0
8	35.71599564357815	37.0	35.0	37.0	33.0	37.0
9	37.42526921733806	39.0	37.0	39.0	35.0	39.0
10	37.262398570895655	39.0	37.0	39.0	34.0	39.0
11	37.37537109326666	39.0	37.0	39.0	34.0	39.0
12	37.27811761880713	39.0	37.0	39.0	34.0	39.0
13	37.3423093068679	39.0	37.0	39.0	34.0	39.0
14	38.475607635149444	40.0	38.0	41.0	34.0	41.0
15	38.45062488943964	40.0	38.0	41.0	34.0	41.0
16	38.37684458215288	40.0	38.0	41.0	34.0	41.0
17	38.35671503520059	40.0	38.0	41.0	34.0	41.0
18	38.31105051359583	40.0	38.0	41.0	34.0	41.0
19	38.3348842335658	40.0	38.0	41.0	34.0	41.0
20	38.215923005995236	40.0	38.0	41.0	34.0	41.0
21	38.13461210614161	40.0	38.0	41.0	33.0	41.0
22	38.062463738493705	40.0	38.0	41.0	33.0	41.0
23	37.953617924480746	40.0	37.0	41.0	33.0	41.0
24	37.83983630650952	40.0	37.0	41.0	33.0	41.0
25	37.760216922861176	40.0	37.0	41.0	32.0	41.0
26	37.40451136675059	39.0	37.0	41.0	32.0	41.0
27	37.18557665075208	39.0	36.0	41.0	31.0	41.0
28	37.01936055096409	39.0	36.0	40.0	31.0	41.0
29	36.970792244597554	39.0	36.0	40.0	31.0	41.0
30	36.888449180730554	39.0	36.0	40.0	30.0	41.0
31	36.66002529254386	39.0	36.0	40.0	30.0	41.0
32	36.54541040463487	39.0	36.0	40.0	30.0	41.0
33	36.36791909593935	39.0	35.0	40.0	30.0	41.0
34	36.218386382446724	39.0	35.0	40.0	29.0	41.0
35	36.05933665617813	39.0	35.0	40.0	29.0	41.0
36	35.88931831117849	38.0	35.0	40.0	27.0	41.0
37	35.660046029771785	38.0	35.0	40.0	27.0	41.0
38	35.549615479091834	38.0	35.0	40.0	26.0	41.0
39	35.37570380547609	38.0	34.0	40.0	25.0	41.0
40	35.242355753675646	38.0	34.0	40.0	25.0	41.0
41	35.091882188714735	38.0	34.0	40.0	24.0	41.0
42	34.89507455319867	38.0	34.0	40.0	24.0	41.0
43	31.615925457800085	35.0	29.0	38.0	15.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	9.0
10	26.0
11	51.0
12	43.0
13	52.0
14	75.0
15	148.0
16	280.0
17	551.0
18	1072.0
19	2162.0
20	4331.0
21	7948.0
22	14145.0
23	22492.0
24	33854.0
25	48585.0
26	66623.0
27	88597.0
28	114408.0
29	144775.0
30	181898.0
31	227903.0
32	283702.0
33	355185.0
34	455745.0
35	598387.0
36	839632.0
37	1321429.0
38	2329966.0
39	1584189.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.530331804812505	19.04275581031921	13.301121505948949	26.125790878919336
2	18.457290017808813	21.97053159711943	34.88803730042996	24.6841410846418
3	18.277930181763523	22.569195890500104	31.690792120861605	27.46208180687477
4	14.475272516963281	16.076644794428766	34.048557651326774	35.39952503728118
5	14.03101464392003	37.945243177795724	34.13470307497574	13.889039103308514
6	35.13192314072993	36.25842435563361	14.260418795765114	14.349233707871347
7	29.315600444716157	31.91400947542375	20.229406443251488	18.54098363660861
8	27.92599994683937	35.63643354508984	18.713503624546647	17.724062883524148
9	27.525462107928906	13.61991342150054	18.295035530547654	40.55958894002289
10	15.32198155326191	28.495849804726348	33.54465446966316	22.63751417234859
11	37.087455191547825	21.431455327199085	21.095764289439458	20.38532519181363
12	21.343511149525266	25.927801908833185	29.925950910742387	22.80273603089916
13	31.96981667832229	19.9427973306032	23.58740523888828	24.499980752186232
14	21.92036125396757	20.682234176234815	26.49640295023157	30.901001619566042
15	25.627937010154593	28.652765315072966	21.824775235946117	23.894522438826325
16	24.009791637833136	26.674743110428373	24.52381137875756	24.791653872980927
17	24.200860560587994	26.391078455005484	25.33880735046511	24.06925363394141
18	23.899666646196767	26.03820186923769	26.024785684759305	24.03734579980624
19	25.45470668623222	25.5577054039612	26.23690117530817	22.75068673449841
20	24.46220691766427	25.436673317855647	26.01620436778723	24.08491539669286
21	24.218584589100423	25.823920999639792	26.533145651873042	23.424348759386746
22	24.9413285391001	25.270053701400414	25.468592609022828	24.32002515047666
23	24.037907194374505	25.57612831142596	25.999247960419165	24.386716533780373
24	23.704942930232175	25.364860641245496	26.277046615455262	24.653149813067067
25	24.1800545904661	24.960553438805242	26.03839663878178	24.82099533194688
26	23.71429186834862	25.345211831356156	26.504182274963267	24.436314025331956
27	24.59799566099284	25.736400732150173	25.836477906717764	23.829125700139226
28	23.825654219441574	26.200742782298974	25.643804999482146	24.329797998777305
29	23.93686762911846	26.22238511575727	26.080042950121584	23.76070430500269
30	24.62222728368436	25.908565552096043	25.510364947714688	23.95884221650491
31	24.114268312690818	25.213937158637734	26.2489539729779	24.422840555693547
32	23.60055791163054	25.86154589274568	26.001092542572042	24.53680365305174
33	23.836801911582874	24.97080748245012	26.25054650042666	24.941844105540344
34	24.069345290197454	25.187494328769155	26.93783093636948	23.80532944466391
35	23.996902476827007	25.10424753421757	26.469238327346652	24.429611661608767
36	23.58466700823898	24.739134838267955	27.12400770645801	24.55219044703506
37	23.279783929542003	24.596322934320046	27.31445794948457	24.809435186653385
38	23.331226003246464	24.159179878152173	27.60267104661362	24.90692307198774
39	22.69827081307348	24.20775769385527	28.460550689117557	24.633420803953683
40	23.257396889003356	23.727032087938678	28.610672179484947	24.404898843573015
41	22.522806367910047	23.928756050458603	29.00048623643831	24.54795134519304
42	22.056585364512348	24.135372165644853	29.02011213226364	24.787930337579155
43	21.9473425643404	23.271706721978163	28.74415805938042	26.036792654301017
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2058.0
1	1940.0
2	1822.0
3	3813.0
4	5804.0
5	5804.0
6	6642.5
7	7481.0
8	8063.0
9	8645.0
10	12611.0
11	16577.0
12	16577.0
13	30277.0
14	43977.0
15	71704.5
16	99432.0
17	77327.5
18	55223.0
19	55223.0
20	64714.5
21	74206.0
22	68328.0
23	62450.0
24	75378.0
25	88306.0
26	88306.0
27	106511.0
28	124716.0
29	142853.0
30	160990.0
31	185445.0
32	209900.0
33	209900.0
34	237996.5
35	266093.0
36	300153.0
37	334213.0
38	371704.5
39	409196.0
40	409196.0
41	450028.0
42	490860.0
43	529896.5
44	568933.0
45	615464.0
46	661995.0
47	661995.0
48	704352.5
49	746710.0
50	766359.0
51	786008.0
52	768763.0
53	751518.0
54	751518.0
55	705446.5
56	659375.0
57	611065.5
58	562756.0
59	508951.5
60	455147.0
61	455147.0
62	399003.0
63	342859.0
64	294443.5
65	246028.0
66	205220.5
67	164413.0
68	164413.0
69	135870.0
70	107327.0
71	90146.0
72	72965.0
73	61021.5
74	49078.0
75	49078.0
76	41426.5
77	33775.0
78	28264.5
79	22754.0
80	18130.5
81	13507.0
82	13507.0
83	11052.5
84	8598.0
85	6878.0
86	5158.0
87	4538.5
88	3919.0
89	3919.0
90	3000.0
91	2081.0
92	1457.0
93	833.0
94	615.0
95	397.0
96	397.0
97	256.5
98	116.0
99	90.5
100	65.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	8728264.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.7265350520725
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.32633409563299	44.23108674497656
2	9.144763490145118	9.82632912392389
3	3.0231778064274875	4.872746051570221
4	1.4386484622615432	3.0917438814122002
5	0.8626701213919006	2.317413825766879
6	0.5783669408007055	1.8644191030733428
7	0.395519069500183	1.4874908405885279
8	0.3062197679414211	1.3161701676753812
9	0.2343633776783689	1.1332379003183093
>10	1.4853919754707563	15.206840174660078
>50	0.13569348577217838	5.057656224043005
>100	0.06498978224782563	6.1969371720919115
>500	0.002645856038180853	0.9663994226025726
>1k	0.0011304516157382377	0.9567748731835407
>5k	0.0	0.0
>10k+	8.531707580692408E-5	1.4747544941136044
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	64132	0.7347623765733942	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	25286	0.2897025112897593	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	20019	0.22935832371706447	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	19256	0.2206166082969076	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.1457032005448048E-5
2	0.0	0.0	0.0	1.1457032005448048E-5	1.1457032005448048E-5
3	0.0	0.0	0.0	1.1457032005448048E-5	1.1457032005448048E-5
4	0.0	0.0	0.0	1.1457032005448048E-5	1.1457032005448048E-5
5	0.0	2.2914064010896096E-5	0.0	1.1457032005448048E-5	1.1457032005448048E-5
6	0.0	2.2914064010896096E-5	0.0	1.1457032005448048E-5	4.582812802179219E-5
7	0.0	2.2914064010896096E-5	0.0	1.1457032005448048E-5	4.582812802179219E-5
8	0.0	2.2914064010896096E-5	0.0	1.1457032005448048E-5	8.019922403813633E-5
9	0.0	2.2914064010896096E-5	0.0	2.2914064010896096E-5	9.165625604358438E-5
10	0.0	2.2914064010896096E-5	0.0	6.874219203268828E-5	1.0311328804903243E-4
11	5.7285160027240236E-5	2.2914064010896096E-5	0.0	9.165625604358438E-5	1.0311328804903243E-4
12	5.7285160027240236E-5	3.437109601634414E-5	0.0	1.0311328804903243E-4	1.0311328804903243E-4
13	5.7285160027240236E-5	3.437109601634414E-5	0.0	1.0311328804903243E-4	1.0311328804903243E-4
14	9.165625604358438E-5	4.582812802179219E-5	0.0	1.489414160708246E-4	1.6039844807627266E-4
15	1.0311328804903243E-4	4.582812802179219E-5	0.0	2.1768360810351292E-4	1.7185548008172072E-4
16	1.0311328804903243E-4	4.582812802179219E-5	0.0	4.3536721620702584E-4	2.1768360810351292E-4
17	1.0311328804903243E-4	4.582812802179219E-5	0.0	6.186797282941946E-4	2.1768360810351292E-4
18	1.0311328804903243E-4	5.7285160027240236E-5	0.0	6.874219203268829E-4	2.1768360810351292E-4
19	1.0311328804903243E-4	5.7285160027240236E-5	0.0	9.62390688457636E-4	2.2914064010896095E-4
20	1.1457032005448047E-4	6.874219203268828E-5	0.0	0.0015352422887300384	2.2914064010896095E-4
21	1.2602735205992854E-4	6.874219203268828E-5	0.0	0.0024747189131767784	2.40597672114409E-4
22	1.2602735205992854E-4	6.874219203268828E-5	0.0	0.004135988553966745	2.40597672114409E-4
23	1.2602735205992854E-4	6.874219203268828E-5	0.0	0.005041094082397141	2.749687681307531E-4
24	1.2602735205992854E-4	6.874219203268828E-5	0.0	0.0065419652751108354	2.749687681307531E-4
25	1.2602735205992854E-4	6.874219203268828E-5	0.0	0.007206473131426822	2.864258001362012E-4
26	1.2602735205992854E-4	6.874219203268828E-5	0.0	0.008340719299966179	2.864258001362012E-4
27	1.2602735205992854E-4	6.874219203268828E-5	0.0	0.013954664982635723	2.864258001362012E-4
28	1.2602735205992854E-4	6.874219203268828E-5	0.0	0.04758105391862574	2.864258001362012E-4
29	1.2602735205992854E-4	6.874219203268828E-5	0.0	0.12147891035376565	2.864258001362012E-4
30	1.489414160708246E-4	6.874219203268828E-5	0.0	0.21881785427205228	2.864258001362012E-4
31	1.489414160708246E-4	6.874219203268828E-5	0.0	0.6391763585519411	2.864258001362012E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTATAC	6515	0.0	20.388334	37
TAGATCG	320	0.0	16.765625	5
GGTATCA	14585	0.0	16.502228	1
TACTTAC	2370	0.0	16.080168	31
AACGCAG	27910	0.0	16.06073	5
TCTTATA	11505	0.0	15.549326	37
GTACTTA	2625	0.0	15.293334	30
TCGTTAC	1810	0.0	15.127071	23
CGCAGAG	31195	0.0	14.36945	7
ACGCAGA	31625	0.0	14.168222	6
AGTGTAC	3305	0.0	14.049925	27
ATCAACG	31870	0.0	14.01867	2
TCAACGC	32100	0.0	13.964331	3
CAACGCA	32175	0.0	13.93178	4
CGCAATC	545	0.0	13.917431	22
TATCAAC	32095	0.0	13.868516	1
AGAGTAC	32260	0.0	13.814787	10
CAGAGTA	32795	0.0	13.74737	9
TTCGTTA	515	0.0	13.650485	22
GTGTACT	3135	0.0	13.631579	28
>>END_MODULE