FastQCFastQC Report
Thu 9 Feb 2017
SRR2727342.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727342.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7298376
Sequences flagged as poor quality0
Sequence length43
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT224190.3071779256097521No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT82380.11287442576266282No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC43950.019.40500637
TCTTATA75050.015.62824837
GGTATCA73550.015.393611
TCGTTAC6300.015.26984223
AACGCAG123700.014.8059825
TACTTAC10900.013.91743131
CGTATAC2800.013.8750013
CGTTACG5850.013.5982924
ACGCAGA138750.013.2400026
TATACCG4100.013.0853665
CTCGTTA7400.013.022
TATACTG10300.012.9320385
GTACTTA12450.012.92771130
AGAGTAC141500.012.89116710
ATACCCG2305.7661964E-1012.8695655
CGCAGAG144250.012.8377827
CAACGCA142650.012.8261484
TCAACGC142800.012.8256313
ATCAACG142100.012.7846592
CAGAGTA147650.012.5546919
Produced by FastQC (version 0.11.5)