##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727342.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7298376
Sequences flagged as poor quality	0
Sequence length	43
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.11462152676157	33.0	31.0	34.0	30.0	34.0
2	32.26832133614382	34.0	31.0	34.0	30.0	34.0
3	32.34860878639303	34.0	31.0	34.0	30.0	34.0
4	35.929897281258185	37.0	35.0	37.0	35.0	37.0
5	35.812656404657695	37.0	35.0	37.0	35.0	37.0
6	35.826320951400696	37.0	35.0	37.0	35.0	37.0
7	35.776189113852176	37.0	35.0	37.0	35.0	37.0
8	35.74235747788275	37.0	35.0	37.0	35.0	37.0
9	37.47251895490175	39.0	37.0	39.0	35.0	39.0
10	37.30795330358425	39.0	37.0	39.0	34.0	39.0
11	37.411594716413624	39.0	37.0	39.0	35.0	39.0
12	37.31411193394256	39.0	37.0	39.0	34.0	39.0
13	37.37315369885027	39.0	37.0	39.0	34.0	39.0
14	38.54885771848422	40.0	38.0	41.0	34.0	41.0
15	38.51290108922862	40.0	38.0	41.0	34.0	41.0
16	38.4403681038083	40.0	38.0	41.0	34.0	41.0
17	38.396312549531565	40.0	38.0	41.0	34.0	41.0
18	38.344507188996566	40.0	38.0	41.0	34.0	41.0
19	38.3498782468867	40.0	38.0	41.0	34.0	41.0
20	38.22855331103796	40.0	38.0	41.0	34.0	41.0
21	38.146586172047044	40.0	38.0	41.0	33.0	41.0
22	38.074464511009026	40.0	38.0	41.0	33.0	41.0
23	37.96269635875159	40.0	37.0	41.0	33.0	41.0
24	37.85341547215435	40.0	37.0	41.0	33.0	41.0
25	37.76865058747316	40.0	37.0	41.0	32.0	41.0
26	37.406017174231636	39.0	37.0	41.0	32.0	41.0
27	37.19554350173244	39.0	37.0	41.0	31.0	41.0
28	37.039945050789385	39.0	36.0	40.0	31.0	41.0
29	37.004762429340445	39.0	36.0	40.0	31.0	41.0
30	36.93299728597157	39.0	36.0	40.0	30.0	41.0
31	36.729096856615776	39.0	36.0	40.0	30.0	41.0
32	36.62686562051613	39.0	36.0	40.0	30.0	41.0
33	36.45331331244101	39.0	36.0	40.0	30.0	41.0
34	36.31681705628759	39.0	35.0	40.0	30.0	41.0
35	36.170107021068794	39.0	35.0	40.0	29.0	41.0
36	36.02300210348165	39.0	35.0	40.0	28.0	41.0
37	35.79514209736522	38.0	35.0	40.0	27.0	41.0
38	35.695745053420104	38.0	35.0	40.0	27.0	41.0
39	35.5339449488489	38.0	35.0	40.0	26.0	41.0
40	35.4121056519971	38.0	34.0	40.0	26.0	41.0
41	35.28577617815251	38.0	34.0	40.0	25.0	41.0
42	35.09906162686055	38.0	34.0	40.0	24.0	41.0
43	31.643166233145564	35.0	29.0	38.0	15.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	14.0
11	18.0
12	29.0
13	26.0
14	53.0
15	89.0
16	194.0
17	361.0
18	773.0
19	1487.0
20	3151.0
21	6199.0
22	10868.0
23	18236.0
24	27588.0
25	39620.0
26	54063.0
27	71544.0
28	92371.0
29	119168.0
30	149924.0
31	186673.0
32	232100.0
33	291956.0
34	374308.0
35	493463.0
36	688178.0
37	1075823.0
38	1967883.0
39	1392215.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.326470984777984	18.315499228869545	12.405568033217254	26.95246175313522
2	18.987826880938993	20.65655428002065	34.25381208093417	26.10180675810619
3	18.623101906506324	21.52696709514555	30.60517846710008	29.244752531248047
4	14.966904966255507	15.433501918783026	33.18116249423159	36.41843062072987
5	14.693104328963047	36.686188817895925	34.0244048813051	14.596301971835926
6	36.87381960041522	34.82535018749376	13.928002613184084	14.372827598906934
7	30.68362057531703	30.76729672464121	19.628859899791408	18.920222800250357
8	28.35877460958438	34.809538450745755	18.388803207727307	18.44288373194256
9	27.520286704877904	13.291532253202632	17.58487093567117	41.603310106248294
10	15.765548390491254	27.35879872453817	33.40634957694698	23.46930330802359
11	38.07784087857353	20.571919013216093	20.649333495561205	20.70090661264917
12	22.06240127940791	24.570945645990285	29.406487141797022	23.960165932804777
13	32.27596111792541	19.188707186365843	23.033124081302468	25.502207614406274
14	22.84808291598021	19.594578300706896	24.856981881996763	32.70035690131613
15	26.246962337922845	27.518080734673028	20.882727883573	25.35222904383112
16	25.55705543260583	25.734437359763323	23.247308716350048	25.4611984912808
17	25.14512817646008	25.670847870814	24.284416149565327	24.899607803160592
18	25.055176658478544	25.218706188883665	24.755740181103302	24.97037697153449
19	26.30653723513286	24.890249556887724	24.714100780776434	24.089112427202984
20	25.709883952265542	24.634713256757394	24.517399487228392	25.13800330374867
21	25.46798082203493	24.87558876111617	24.716087524128653	24.940342892720242
22	26.09106464232591	24.45581044330958	24.04963789204612	25.403487022318387
23	25.42268307360432	24.575453498148082	24.38558934206733	25.61627408618027
24	25.281665400631592	24.45206988513609	24.50227283439494	25.763991879837377
25	25.619069228551666	24.073026656889148	24.434531736923393	25.87337237763579
26	25.192618193417278	24.429873166304393	24.792652502419717	25.584856137858615
27	25.773569352962905	24.726267871098994	24.339154354338554	25.161008421599547
28	25.402527904838006	24.93127238169149	24.26442540093851	25.401774312531995
29	25.325017510744857	25.096733848735663	24.51646777310459	25.06178086741489
30	25.74623450477202	24.83257919296019	24.158812864670168	25.262373437597624
31	25.433219664210228	24.31976647955655	24.58479804274266	25.662215813490562
32	25.052354112750564	24.711675583718897	24.548817435550045	25.687152867980494
33	25.176696843242937	24.215551514473905	24.58096157282113	26.02679006946203
34	25.35494197613277	24.16189574228568	25.162323782715497	25.32083849886605
35	25.239327214711878	24.145083783022415	24.973487252506583	25.64210174975912
36	24.99979447482563	23.838727958110134	25.423107825631348	25.738369741432887
37	24.747697295946384	23.558775267264938	25.69426403901361	25.999263397775067
38	24.565656798169893	23.407042881868513	25.952526973123884	26.07477334683771
39	24.188257771317893	23.32695657225662	26.68616963554632	25.798616020879166
40	24.543035327311173	22.972864100177905	26.8724165485582	25.611684023952723
41	23.971510922429868	23.023080751115042	27.233825716844407	25.77158260961069
42	23.56724290444888	23.15278083781926	27.316350925192122	25.96362533253973
43	23.59200183712103	22.320554600091853	27.17756662578086	26.90987693700626
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	240.0
1	329.0
2	418.0
3	1103.5
4	1789.0
5	1789.0
6	2096.5
7	2404.0
8	2498.5
9	2593.0
10	3778.5
11	4964.0
12	4964.0
13	9550.5
14	14137.0
15	23935.5
16	33734.0
17	25972.5
18	18211.0
19	18211.0
20	22108.0
21	26005.0
22	25320.5
23	24636.0
24	31826.0
25	39016.0
26	39016.0
27	49917.0
28	60818.0
29	73625.0
30	86432.0
31	105301.5
32	124171.0
33	124171.0
34	147885.0
35	171599.0
36	202267.5
37	232936.0
38	269550.5
39	306165.0
40	306165.0
41	347138.5
42	388112.0
43	431425.5
44	474739.0
45	520122.5
46	565506.0
47	565506.0
48	607395.5
49	649285.0
50	674041.5
51	698798.0
52	692470.5
53	686143.0
54	686143.0
55	656756.0
56	627369.0
57	587756.5
58	548144.0
59	504131.5
60	460119.0
61	460119.0
62	404395.5
63	348672.0
64	297553.5
65	246435.0
66	205525.5
67	164616.0
68	164616.0
69	135106.0
70	105596.0
71	86927.5
72	68259.0
73	56347.0
74	44435.0
75	44435.0
76	36262.0
77	28089.0
78	23155.5
79	18222.0
80	14504.5
81	10787.0
82	10787.0
83	8639.5
84	6492.0
85	5077.0
86	3662.0
87	3096.0
88	2530.0
89	2530.0
90	1886.0
91	1242.0
92	884.5
93	527.0
94	380.0
95	233.0
96	233.0
97	148.5
98	64.0
99	48.0
100	32.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	7298376.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.84482769901599
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.89338840598465	45.174773375217896
2	9.759295464472753	10.900123473545381
3	3.30307996004915	5.533797937350521
4	1.6729884493925096	3.737110067950745
5	0.9685398001095685	2.704396912837912
6	0.6531076717948313	2.1883611240172702
7	0.45436100321215184	1.7761598356274266
8	0.3212687521223138	1.4352958485878793
9	0.25754672968306624	1.2944387469236291
>10	1.5585481570440147	16.178680555890654
>50	0.11610969944707604	4.40818019658075
>100	0.040637359049110484	3.64586156310073
>500	9.077665385634572E-4	0.3363969778071863
>1k	1.226561676545524E-4	0.08666256306684902
>5k	7.35936994533427E-5	0.2926334149830881
>10k+	2.4531233151114235E-5	0.30712740651214143
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	22419	0.3071779256097521	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	8238	0.11287442576266282	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.740335658234106E-5
2	0.0	1.370167829117053E-5	0.0	0.0	2.740335658234106E-5
3	0.0	1.370167829117053E-5	0.0	0.0	2.740335658234106E-5
4	0.0	1.370167829117053E-5	0.0	0.0	2.740335658234106E-5
5	0.0	1.370167829117053E-5	0.0	0.0	2.740335658234106E-5
6	0.0	1.370167829117053E-5	0.0	0.0	8.221006974702317E-5
7	0.0	1.370167829117053E-5	0.0	0.0	9.591174803819371E-5
8	1.370167829117053E-5	1.370167829117053E-5	0.0	0.0	9.591174803819371E-5
9	1.370167829117053E-5	1.370167829117053E-5	0.0	0.0	1.5071846120287582E-4
10	5.480671316468212E-5	1.370167829117053E-5	0.0	0.0	1.5071846120287582E-4
11	1.0961342632936424E-4	1.370167829117053E-5	0.0	0.0	1.5071846120287582E-4
12	1.0961342632936424E-4	1.370167829117053E-5	0.0	2.740335658234106E-5	1.5071846120287582E-4
13	1.0961342632936424E-4	1.370167829117053E-5	0.0	2.740335658234106E-5	1.5071846120287582E-4
14	1.2331510462053476E-4	1.370167829117053E-5	0.0	4.1105034873511583E-5	2.4663020924106953E-4
15	1.2331510462053476E-4	4.1105034873511583E-5	0.0	9.591174803819371E-5	2.6033188753224007E-4
16	1.5071846120287582E-4	4.1105034873511583E-5	0.0	2.3292853094989899E-4	3.5624363557043375E-4
17	1.5071846120287582E-4	4.1105034873511583E-5	0.0	4.6585706189979797E-4	3.699453138616043E-4
18	1.5071846120287582E-4	4.1105034873511583E-5	0.0	4.795587401909685E-4	4.1105034873511586E-4
19	1.6442013949404633E-4	4.1105034873511583E-5	0.0	6.165755231026738E-4	4.247520270262864E-4
20	2.1922685265872847E-4	4.1105034873511583E-5	0.0	8.906090889260844E-4	4.5215538360862743E-4
21	2.1922685265872847E-4	4.1105034873511583E-5	0.0	0.0013564661508258823	4.795587401909685E-4
22	2.3292853094989899E-4	4.1105034873511583E-5	0.0	0.0025348104838665477	4.795587401909685E-4
23	2.3292853094989899E-4	4.1105034873511583E-5	0.0	0.0030143692240575163	5.480671316468211E-4
24	2.3292853094989899E-4	4.1105034873511583E-5	0.0	0.004124205165642329	5.480671316468211E-4
25	2.3292853094989899E-4	4.1105034873511583E-5	0.0	0.004494150479503934	5.754704882291622E-4
26	2.3292853094989899E-4	4.1105034873511583E-5	0.0	0.005288847820391824	6.028738448115033E-4
27	2.3292853094989899E-4	4.1105034873511583E-5	0.0	0.009399351307742982	6.028738448115033E-4
28	2.3292853094989899E-4	4.1105034873511583E-5	0.0	0.03351430510020311	6.028738448115033E-4
29	2.3292853094989899E-4	5.480671316468212E-5	0.0	0.0902392532256491	6.028738448115033E-4
30	2.3292853094989899E-4	5.480671316468212E-5	0.0	0.1647763831296168	6.028738448115033E-4
31	2.3292853094989899E-4	5.480671316468212E-5	0.0	0.4683644690270822	6.028738448115033E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTATAC	4395	0.0	19.405006	37
TCTTATA	7505	0.0	15.628248	37
GGTATCA	7355	0.0	15.39361	1
TCGTTAC	630	0.0	15.269842	23
AACGCAG	12370	0.0	14.805982	5
TACTTAC	1090	0.0	13.917431	31
CGTATAC	280	0.0	13.875001	3
CGTTACG	585	0.0	13.59829	24
ACGCAGA	13875	0.0	13.240002	6
TATACCG	410	0.0	13.085366	5
CTCGTTA	740	0.0	13.0	22
TATACTG	1030	0.0	12.932038	5
GTACTTA	1245	0.0	12.927711	30
AGAGTAC	14150	0.0	12.891167	10
ATACCCG	230	5.7661964E-10	12.869565	5
CGCAGAG	14425	0.0	12.837782	7
CAACGCA	14265	0.0	12.826148	4
TCAACGC	14280	0.0	12.825631	3
ATCAACG	14210	0.0	12.784659	2
CAGAGTA	14765	0.0	12.554691	9
>>END_MODULE