FastQCFastQC Report
Thu 9 Feb 2017
SRR2727341.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727341.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10483900
Sequences flagged as poor quality0
Sequence length43
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT325960.3109148313127748No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT139930.1334713226947987No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT115330.11000677228893828No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC71400.021.45378337
GGTATCA68950.018.8353881
AACGCAG156350.016.2932515
TCTTATA129300.016.22505837
TAGATCG4000.016.18755
TCAACGC165950.015.3061173
CAACGCA167400.015.206694
ATCAACG168450.015.0679722
TATCAAC170950.014.7826841
ACGCAGA174250.014.6407456
TATACCG4350.014.459775
CGCAGAG181300.014.1530617
AGAGTAC182550.013.97507610
CAGAGTA192100.013.6366479
GCAGAGT200250.013.1740328
CTCTTAT206500.013.07094437
TCTATAC10000.012.953
GTATCAA100600.012.9463222
TTATACT14800.012.7500014
TAGGTCG4100.012.6341475
Produced by FastQC (version 0.11.5)