FastQCFastQC Report
Thu 9 Feb 2017
SRR2727340.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727340.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7361565
Sequences flagged as poor quality0
Sequence length43
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT199460.2709478215569651No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT76380.10375511185461243No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC46800.021.66239237
TCTTATA86700.016.5155737
AACGCAG114500.015.6886465
GGTATCA60750.014.8304531
ACGCAGA128650.014.0781186
TCAACGC128600.013.9397363
CAACGCA128850.013.8983314
ATCAACG129900.013.7147812
TACTTAC9450.013.70370331
AGAGTAC131350.013.66197210
CGCAGAG133950.013.6039577
TATCAAC131900.013.3946181
CTCTTAT135600.013.34291937
GTCTTAG11250.013.3200011
CAGAGTA138250.013.2611229
TATACTG8000.013.1812495
GTAATAC5300.012.9150943
GCAGAGT146300.012.619968
CTAGACA9250.012.5999984
TATACCG3400.012.5147065
Produced by FastQC (version 0.11.5)