##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727339.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3970667
Sequences flagged as poor quality	0
Sequence length	43
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.382377822164386	31.0	31.0	34.0	30.0	34.0
2	31.56137847872914	31.0	31.0	34.0	30.0	34.0
3	31.61907281572592	31.0	31.0	34.0	30.0	34.0
4	35.384838114100226	37.0	35.0	37.0	33.0	37.0
5	35.00865622828608	37.0	35.0	37.0	32.0	37.0
6	35.00747355545051	36.0	35.0	37.0	32.0	37.0
7	34.9775803410359	36.0	35.0	37.0	32.0	37.0
8	34.91091899673279	35.0	35.0	37.0	32.0	37.0
9	36.524983837728016	38.0	35.0	39.0	32.0	39.0
10	36.20485701772523	38.0	35.0	39.0	32.0	39.0
11	36.37382611032353	38.0	35.0	39.0	32.0	39.0
12	36.24557637293684	38.0	35.0	39.0	32.0	39.0
13	36.33912060618531	38.0	35.0	39.0	32.0	39.0
14	37.25215159065215	39.0	36.0	40.0	32.0	41.0
15	37.189670148617346	39.0	36.0	40.0	32.0	41.0
16	37.08619886784764	38.0	36.0	40.0	32.0	41.0
17	37.03222934585046	38.0	36.0	40.0	32.0	41.0
18	36.95570215281211	38.0	36.0	40.0	31.0	41.0
19	36.96712365957659	39.0	36.0	40.0	31.0	41.0
20	36.80715733653817	39.0	36.0	40.0	31.0	41.0
21	36.70251270126656	38.0	36.0	40.0	31.0	41.0
22	36.60387511720323	38.0	36.0	40.0	30.0	41.0
23	36.46915341931217	38.0	35.0	40.0	30.0	41.0
24	36.314568559891825	38.0	35.0	40.0	30.0	41.0
25	36.223616082638	38.0	35.0	40.0	30.0	41.0
26	35.79732699820962	38.0	34.0	40.0	28.0	41.0
27	35.512628482821654	38.0	34.0	40.0	27.0	41.0
28	35.327238975214996	38.0	34.0	40.0	27.0	41.0
29	35.24079833438563	38.0	34.0	40.0	27.0	41.0
30	35.15170247215392	38.0	34.0	40.0	26.0	41.0
31	34.879696786459306	38.0	33.0	40.0	25.0	41.0
32	34.75426244507535	38.0	33.0	40.0	25.0	40.0
33	34.54648350012731	38.0	33.0	40.0	24.0	40.0
34	34.35821815327249	37.0	33.0	40.0	24.0	40.0
35	34.18120230177952	37.0	33.0	40.0	24.0	40.0
36	33.989894393057895	37.0	32.0	39.0	23.0	40.0
37	33.71254703554843	37.0	32.0	39.0	22.0	40.0
38	33.58282827545095	37.0	31.0	39.0	22.0	40.0
39	33.35264604158445	37.0	31.0	39.0	20.0	40.0
40	33.217261986462226	37.0	31.0	39.0	20.0	40.0
41	33.03356866743044	36.0	31.0	39.0	19.0	40.0
42	32.81105189631868	36.0	30.0	39.0	18.0	40.0
43	29.74604543770606	33.0	25.0	38.0	9.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	2.0
10	20.0
11	32.0
12	26.0
13	57.0
14	115.0
15	264.0
16	467.0
17	783.0
18	1532.0
19	2904.0
20	5069.0
21	8624.0
22	13680.0
23	20741.0
24	29470.0
25	39994.0
26	52563.0
27	68048.0
28	85857.0
29	106510.0
30	131053.0
31	159282.0
32	196110.0
33	241634.0
34	307106.0
35	402958.0
36	553965.0
37	737904.0
38	677432.0
39	126464.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.75733371748374	19.88673439500215	13.413741318524067	25.942190568990046
2	19.403893602762455	22.319172068571856	34.042643213344256	24.23429111532143
3	18.799284855667825	22.75272643110087	31.52850641970228	26.919482293529022
4	15.149772066003017	16.710315924251518	33.66162410496775	34.47828790477771
5	14.192300689027812	37.78962073626421	34.03808478525144	13.979993789456532
6	35.19645439922311	36.64744487513055	14.049553891071701	14.106546834574646
7	29.49640450836094	32.09740328262229	19.873713912549203	18.532478296467573
8	28.064277361964628	35.55254066885992	18.417837607636198	17.965344361539255
9	27.959534254572343	13.376039844187387	17.757797367545553	40.906628533694715
10	15.41282610704952	28.415704464766243	33.41839041148502	22.75307901669921
11	37.92584470065105	21.087792051058425	20.826576492060404	20.159786756230126
12	21.753926985063217	25.715478029258055	29.491569048726575	23.039025936952154
13	32.405512726199404	19.995129281805802	23.066426874880218	24.532931117114583
14	22.113790957539376	20.798596306363642	25.83606230389	31.251550432206983
15	26.075921249502915	28.539512379154434	21.325006604683796	24.05955976665885
16	24.547714527559222	26.913916478012386	23.906159846695783	24.63220914773261
17	25.0046150936354	26.410524982326645	24.311003667645764	24.273856256392186
18	24.57126220859115	25.830546857744558	25.23210835862086	24.36608257504344
19	26.349804705355552	25.397697666412217	25.26326181470267	22.989235813529564
20	25.053977077402866	25.224880353854907	25.208762155073693	24.512380413668534
21	24.89770610328189	25.592803425721673	25.84019259232769	23.669297878668747
22	25.52122854925885	25.275627495330127	24.579573154837714	24.623570800573304
23	24.724964344781366	25.3856593866975	25.197957924953162	24.69141834356797
24	24.3495362366071	25.33367819562809	25.35367483599103	24.96311073177378
25	24.75375044041719	24.830765208968668	25.349267516011793	25.066216834602344
26	24.361221930723477	25.18619667678	25.565729888706358	24.88685150379017
27	25.22102709695877	25.686163055224725	24.92953954587479	24.163270301941715
28	24.398822666317773	25.972235898905648	24.77807884670258	24.850862588073994
29	24.67361277085185	26.01857572040164	25.098125831252027	24.209685677494484
30	25.2029444927011	25.788765464341378	24.60140827724914	24.40688176570838
31	24.88916849486497	24.995397498707398	25.269356508616813	24.84607749781082
32	24.284912333368673	25.752675809882824	24.9994270484027	24.9629848083458
33	24.543962009405472	24.706226938698208	25.39601029247731	25.35380075941901
34	24.799460644773283	25.010609048807165	26.03393837861498	24.155991927804575
35	24.76838274274826	24.95681456037487	25.538555612948656	24.736247083928216
36	24.287632279413003	24.54801674378637	26.103725142400506	25.060625834400113
37	24.08310744769078	24.602642326843323	26.210936348981168	25.103313876484734
38	24.22671052495714	24.063992271323684	26.451072326135634	25.25822487758354
39	23.575761956366527	24.221623218466824	27.126273746954855	25.076341078211794
40	24.150778697886274	23.57520789328342	27.417786482724438	24.856226926105865
41	23.46915518224016	23.703700159192397	27.83582707892654	24.9913175796409
42	23.00628584567782	23.8470765742884	27.73944528715201	25.407192292881774
43	23.000971877017136	22.943676717287044	27.554614879565577	26.500736526130243
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	622.0
1	567.5
2	513.0
3	917.0
4	1321.0
5	1321.0
6	1634.5
7	1948.0
8	2261.0
9	2574.0
10	3649.5
11	4725.0
12	4725.0
13	8505.0
14	12285.0
15	20434.5
16	28584.0
17	23622.5
18	18661.0
19	18661.0
20	22204.0
21	25747.0
22	25420.0
23	25093.0
24	30262.0
25	35431.0
26	35431.0
27	42977.5
28	50524.0
29	58946.0
30	67368.0
31	78969.0
32	90570.0
33	90570.0
34	102692.0
35	114814.0
36	129522.5
37	144231.0
38	161312.0
39	178393.0
40	178393.0
41	201059.0
42	223725.0
43	244077.0
44	264429.0
45	285974.0
46	307519.0
47	307519.0
48	327576.5
49	347634.0
50	357206.5
51	366779.0
52	357400.0
53	348021.0
54	348021.0
55	328284.0
56	308547.0
57	288715.0
58	268883.0
59	244195.5
60	219508.0
61	219508.0
62	191504.0
63	163500.0
64	139164.0
65	114828.0
66	95548.5
67	76269.0
68	76269.0
69	62678.0
70	49087.0
71	41301.5
72	33516.0
73	28510.5
74	23505.0
75	23505.0
76	20477.5
77	17450.0
78	14885.0
79	12320.0
80	10287.5
81	8255.0
82	8255.0
83	6740.0
84	5225.0
85	4286.5
86	3348.0
87	3003.5
88	2659.0
89	2659.0
90	2013.5
91	1368.0
92	989.0
93	610.0
94	400.5
95	191.0
96	191.0
97	129.5
98	68.0
99	43.5
100	19.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	3970667.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	64.24927625346099
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.03618726278327	55.27762763241064
2	7.561301603233255	9.716163110837416
3	2.3249868979621438	4.481361764785414
4	1.1723881343457576	3.0130035647944124
5	0.6621779016508176	2.1272225466050245
6	0.441952622354773	1.7037081674767998
7	0.3109128693021316	1.3983148781384203
8	0.23221919301554309	1.1935932066729125
9	0.17153115402582414	0.9918677250972129
>10	0.9965847913073346	11.510325443358264
>50	0.05819518749104047	2.5404300644978335
>100	0.028581117122828226	3.414615808994675
>500	0.0018436773282143116	0.8552698140734264
>1k	9.806793768092036E-4	0.8628811394055736
>5k	1.1768152521709971E-4	0.4710473609104092
>10k+	3.9227175072366566E-5	0.4425677719415081
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	17560	0.44224307905951316	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	7698	0.19387170971526949	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	5788	0.14576896022758895	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5204	0.1310611038397327	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.5184685595644257E-5
2	0.0	0.0	0.0	0.0	2.5184685595644257E-5
3	0.0	0.0	0.0	0.0	2.5184685595644257E-5
4	0.0	0.0	0.0	0.0	2.5184685595644257E-5
5	0.0	0.0	0.0	0.0	2.5184685595644257E-5
6	2.5184685595644257E-5	0.0	0.0	0.0	5.036937119128851E-5
7	5.036937119128851E-5	0.0	0.0	0.0	5.036937119128851E-5
8	5.036937119128851E-5	0.0	0.0	0.0	5.036937119128851E-5
9	5.036937119128851E-5	0.0	0.0	0.0	5.036937119128851E-5
10	1.2592342797822128E-4	0.0	0.0	0.0	5.036937119128851E-5
11	2.2666217036079834E-4	0.0	0.0	0.0	5.036937119128851E-5
12	2.2666217036079834E-4	0.0	0.0	2.5184685595644257E-5	5.036937119128851E-5
13	2.2666217036079834E-4	2.5184685595644257E-5	0.0	2.5184685595644257E-5	5.036937119128851E-5
14	2.2666217036079834E-4	2.5184685595644257E-5	0.0	5.036937119128851E-5	7.555405678693277E-5
15	2.2666217036079834E-4	2.5184685595644257E-5	0.0	7.555405678693277E-5	7.555405678693277E-5
16	2.2666217036079834E-4	2.5184685595644257E-5	0.0	3.022162271477311E-4	1.2592342797822128E-4
17	2.2666217036079834E-4	2.5184685595644257E-5	0.0	4.533243407215967E-4	1.2592342797822128E-4
18	2.2666217036079834E-4	2.5184685595644257E-5	0.0	6.044324542954622E-4	1.2592342797822128E-4
19	2.5184685595644257E-4	2.5184685595644257E-5	0.0	7.303558822736835E-4	1.2592342797822128E-4
20	2.5184685595644257E-4	2.5184685595644257E-5	0.0	0.0011081261662083473	1.2592342797822128E-4
21	2.770315415520868E-4	2.5184685595644257E-5	0.0	0.0017125586205038097	1.5110811357386554E-4
22	3.022162271477311E-4	2.5184685595644257E-5	0.0	0.0028710541579034456	1.5110811357386554E-4
23	3.022162271477311E-4	2.5184685595644257E-5	0.0	0.0034251172410076193	2.0147748476515405E-4
24	3.022162271477311E-4	2.5184685595644257E-5	0.0	0.004382135293642101	2.0147748476515405E-4
25	3.2740091274337534E-4	2.5184685595644257E-5	0.0	0.004608797464002899	2.0147748476515405E-4
26	3.2740091274337534E-4	2.5184685595644257E-5	0.0	0.005515446145446093	2.0147748476515405E-4
27	3.2740091274337534E-4	2.5184685595644257E-5	0.0	0.00982202738230126	2.0147748476515405E-4
28	3.2740091274337534E-4	5.036937119128851E-5	0.0	0.034326726466863126	2.0147748476515405E-4
29	3.2740091274337534E-4	7.555405678693277E-5	0.0	0.08676124187699447	2.0147748476515405E-4
30	3.2740091274337534E-4	7.555405678693277E-5	0.0	0.15584283446584668	2.0147748476515405E-4
31	3.525855983390196E-4	7.555405678693277E-5	0.0	0.4721876702327342	2.2666217036079834E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	5030	0.0	21.515903	1
CTTATAC	2430	0.0	20.25103	37
TACTTAC	810	0.0	15.759259	31
ACCGTGA	975	0.0	15.558974	8
GTATCAA	6900	0.0	15.523912	2
AACGCAG	11360	0.0	15.454666	5
TAGGACG	145	5.3560143E-8	15.310345	4
TTAGCGC	110	1.453575E-5	15.136364	4
ATCAACG	11580	0.0	15.113127	2
TAGATCG	135	3.9789848E-7	15.074075	5
CAACGCA	11625	0.0	15.022796	4
TCAACGC	11625	0.0	15.022796	3
GACCGTG	1100	0.0	14.968183	7
TCGTTAC	695	0.0	14.906476	23
CAGACCG	910	0.0	14.84066	5
CTAATCG	765	0.0	14.751634	19
TATCAAC	11915	0.0	14.672682	1
TAATCGA	760	0.0	14.605264	20
CGCAGAG	12045	0.0	14.591117	7
GTACTTA	995	0.0	14.502513	30
>>END_MODULE