FastQCFastQC Report
Thu 9 Feb 2017
SRR2727338.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727338.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7224626
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT541690.7497827569205658No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT192630.26662971896399895No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT189120.2617713359833436No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT142980.1979064383401992No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC42250.019.52899437
AACGCAG225700.015.0573775
TCTTATA70700.014.57496537
TATACCG3150.013.5079375
TAAGACT11000.013.4545464
GAGTACT159850.013.3672211
ACGCAGA260250.013.0939486
AGTACTT163300.013.02816912
CGCAGAG265900.012.8574667
CTCTTAT110950.012.72239837
GTACTTT168200.012.68162813
CGTTACG5400.012.67592624
AGAGTAC271050.012.54491710
CAGAGTA276650.012.3846029
TACTTTT174750.012.2486414
GCAGAGT284600.012.1101558
ATACCGT4150.012.0361446
CTCGTTA9100.011.99450622
ACTTTTT182550.011.79622115
TACTTAC12850.011.66147931
Produced by FastQC (version 0.11.5)