##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727338.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7224626
Sequences flagged as poor quality	0
Sequence length	43
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.13128485820581	33.0	31.0	34.0	30.0	34.0
2	32.27418858775527	34.0	31.0	34.0	30.0	34.0
3	32.35862673029718	34.0	31.0	34.0	30.0	34.0
4	35.930003850718364	37.0	35.0	37.0	35.0	37.0
5	35.8125400539765	37.0	35.0	37.0	35.0	37.0
6	35.83348743588942	37.0	35.0	37.0	35.0	37.0
7	35.77790241321834	37.0	35.0	37.0	35.0	37.0
8	35.75366143520786	37.0	35.0	37.0	35.0	37.0
9	37.4777549730602	39.0	37.0	39.0	35.0	39.0
10	37.32413691726049	39.0	37.0	39.0	34.0	39.0
11	37.4288183222218	39.0	37.0	39.0	35.0	39.0
12	37.33831481380489	39.0	37.0	39.0	34.0	39.0
13	37.39456146242034	39.0	37.0	39.0	34.0	39.0
14	38.558716395838346	40.0	38.0	41.0	34.0	41.0
15	38.52127985587074	40.0	38.0	41.0	34.0	41.0
16	38.4532450537924	40.0	38.0	41.0	34.0	41.0
17	38.42594384816598	40.0	38.0	41.0	34.0	41.0
18	38.38667690202925	40.0	38.0	41.0	34.0	41.0
19	38.39844415475625	40.0	38.0	41.0	34.0	41.0
20	38.28768326554205	40.0	38.0	41.0	34.0	41.0
21	38.21327553841542	40.0	38.0	41.0	34.0	41.0
22	38.141612866880585	40.0	38.0	41.0	33.0	41.0
23	38.0282654077872	40.0	38.0	41.0	33.0	41.0
24	37.920314214189084	40.0	37.0	41.0	33.0	41.0
25	37.83851399366556	40.0	37.0	41.0	33.0	41.0
26	37.48270830905295	39.0	37.0	41.0	32.0	41.0
27	37.26886955809201	39.0	37.0	41.0	31.0	41.0
28	37.11158238502588	39.0	36.0	41.0	31.0	41.0
29	37.05759813725998	39.0	36.0	40.0	31.0	41.0
30	36.97442829566541	39.0	36.0	40.0	31.0	41.0
31	36.76039728561727	39.0	36.0	40.0	30.0	41.0
32	36.64413119793329	39.0	36.0	40.0	30.0	41.0
33	36.4587811189119	39.0	35.0	40.0	30.0	41.0
34	36.31723109265449	39.0	35.0	40.0	30.0	41.0
35	36.16244744018583	39.0	35.0	40.0	29.0	41.0
36	36.00165821732502	38.0	35.0	40.0	28.0	41.0
37	35.761654651742525	38.0	35.0	40.0	27.0	41.0
38	35.643547361482796	38.0	35.0	40.0	27.0	41.0
39	35.475113175408666	38.0	35.0	40.0	26.0	41.0
40	35.328499634444746	38.0	34.0	40.0	25.0	41.0
41	35.176707140272725	38.0	34.0	40.0	25.0	41.0
42	34.96732329119874	38.0	34.0	40.0	24.0	41.0
43	31.66242072600021	35.0	29.0	38.0	15.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	4.0
10	33.0
11	46.0
12	47.0
13	46.0
14	56.0
15	133.0
16	180.0
17	403.0
18	856.0
19	1628.0
20	3284.0
21	6308.0
22	10964.0
23	17547.0
24	26713.0
25	38107.0
26	52241.0
27	69467.0
28	90306.0
29	115551.0
30	145422.0
31	181681.0
32	227544.0
33	287419.0
34	370513.0
35	487495.0
36	688827.0
37	1094166.0
38	1934134.0
39	1373505.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.57502049241027	18.6665856474785	12.876209785807596	25.882184074303638
2	18.777207290730345	21.76143097234376	34.12076971181622	25.340592025109675
3	19.06026692592807	22.31787500141876	30.633987143417528	27.987870929235648
4	14.994437082279417	15.63099598512089	32.99416468063537	36.38040225196432
5	14.766854367271055	37.24023914871164	33.54983358308098	14.443072900936324
6	36.48759119157172	34.91351663048025	13.86826113905412	14.730631038893915
7	30.058843184408442	30.866455924500453	20.00023253798882	19.07446835310229
8	28.48831205933705	34.80433450811156	18.49265276846165	18.21470066408974
9	27.137972263200894	13.683158131645845	18.11019698459131	41.068672620561955
10	15.687732486083018	28.04096710334902	33.23174099254411	23.039559418023853
11	37.27120268924647	21.24505822169895	20.703438489411077	20.780300599643496
12	21.692486226968704	25.334543269091025	29.614501844109302	23.358468659830972
13	32.168613295691706	19.53334331770254	23.48244739589288	24.815595990712875
14	22.596353084574897	20.2986424487579	25.693122384466683	31.41188208220052
15	25.894779882031266	28.146522740415907	21.566583515880268	24.392113861672563
16	24.79967821171643	25.99344242871534	23.923785120503123	25.283094239065107
17	24.523553191542373	26.17844854529494	25.21783134517967	24.08016691798302
18	24.2848557143304	25.366641816476037	25.929134601569686	24.419367867623873
19	25.37608175149828	25.472543492216758	25.795425257999515	23.355949498285447
20	24.77501257504541	25.32335929915265	25.70535831197352	24.196269813828426
21	24.55929206577614	25.651985306921077	25.631776648369065	24.15694597893372
22	25.110628564025323	25.154409377039034	25.080094111446044	24.6548679474896
23	24.537685410981826	25.208958913582517	25.37547272343233	24.877882952003326
24	24.407976274481197	25.14437425549779	25.42917792561165	25.01847154440936
25	24.70168005928611	24.952475048535383	25.47601772050207	24.86982717167643
26	24.31458735718638	25.30377351021354	25.75421897271914	24.62742015988094
27	24.882741334983987	25.594141482202676	25.403792528499054	24.119324654314287
28	24.526224610104386	25.80648465401531	25.339913789308955	24.327376946571352
29	24.314019853761287	25.923431884224872	25.667833878182762	24.094714383831082
30	24.636237225290277	25.736045021569282	25.351734470407187	24.27598328273325
31	24.423990944306322	25.171559053714336	25.620952558651478	24.78349744332786
32	24.04251791026968	25.620897192463666	25.531771471630506	24.80481342563615
33	24.32463632027457	25.123667301255452	25.52922462699107	25.022471751478896
34	24.47747468173439	25.153426627205338	25.970022531270132	24.39907615979014
35	24.363046613070352	25.141979667874846	25.829046375549407	24.665927343505395
36	24.080900519971554	24.76423000996868	26.334968758244372	24.819900711815393
37	23.794698853615397	24.589189807195556	26.541664578900004	25.074446760289042
38	23.60513886808812	24.498638407026192	26.969423745948923	24.926798978936766
39	23.310424650355603	24.440490068274816	27.402262760729762	24.846822520639822
40	23.65271226496707	24.206429509292246	27.649043701362537	24.491814524378146
41	23.09960404870785	24.24578102728086	27.8988005745903	24.755814349420994
42	22.84046260664566	24.391075745651055	27.871615776373755	24.896845871329536
43	22.86143255028011	23.693627877761422	27.718472900880958	25.726466671077507
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1670.0
1	1628.5
2	1587.0
3	3452.5
4	5318.0
5	5318.0
6	5938.0
7	6558.0
8	6688.5
9	6819.0
10	10174.0
11	13529.0
12	13529.0
13	26907.0
14	40285.0
15	63054.5
16	85824.0
17	64647.0
18	43470.0
19	43470.0
20	51636.5
21	59803.0
22	51609.0
23	43415.0
24	53221.5
25	63028.0
26	63028.0
27	76432.0
28	89836.0
29	103272.0
30	116708.0
31	133264.0
32	149820.0
33	149820.0
34	169981.5
35	190143.0
36	217951.5
37	245760.0
38	278874.0
39	311988.0
40	311988.0
41	347597.5
42	383207.0
43	419786.5
44	456366.0
45	495453.0
46	534540.0
47	534540.0
48	571705.0
49	608870.0
50	628126.5
51	647383.0
52	642467.5
53	637552.0
54	637552.0
55	600348.0
56	563144.0
57	526966.5
58	490789.0
59	453215.5
60	415642.0
61	415642.0
62	367237.5
63	318833.0
64	272621.0
65	226409.0
66	190520.5
67	154632.0
68	154632.0
69	129155.5
70	103679.0
71	88512.5
72	73346.0
73	60503.5
74	47661.0
75	47661.0
76	39811.0
77	31961.0
78	26808.0
79	21655.0
80	17610.5
81	13566.0
82	13566.0
83	10884.5
84	8203.0
85	6656.5
86	5110.0
87	4273.0
88	3436.0
89	3436.0
90	2606.5
91	1777.0
92	1279.5
93	782.0
94	575.5
95	369.0
96	369.0
97	238.0
98	107.0
99	76.5
100	46.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	7224626.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.0145427011539
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.46682050741535	44.81859875532442
2	9.64870965674117	10.616386988436464
3	3.196544646586217	5.275693258672871
4	1.557859671829612	3.428197497531032
5	0.921272279602837	2.5341686582799836
6	0.576139700675992	1.9017637298801633
7	0.42695623992600185	1.6442161605053198
8	0.3074766705965024	1.3532550739311933
9	0.22536338736656647	1.1158437327799193
>10	1.5037519383221714	15.552655693555609
>50	0.11989773454426127	4.490284617424244
>100	0.046514761934091285	4.334030977767425
>500	0.001988194602259323	0.7443077823029302
>1k	5.787866697974242E-4	0.6233632579230983
>5k	2.5164637421695652E-5	0.09085955154226731
>10k+	1.0065854968678261E-4	1.4763742641429942
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	54169	0.7497827569205658	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	19263	0.26662971896399895	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	18912	0.2617713359833436	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	14298	0.1979064383401992	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	4.152464086030197E-5
2	0.0	0.0	0.0	0.0	4.152464086030197E-5
3	0.0	0.0	0.0	0.0	4.152464086030197E-5
4	0.0	0.0	0.0	0.0	4.152464086030197E-5
5	1.384154695343399E-5	0.0	0.0	0.0	4.152464086030197E-5
6	2.768309390686798E-5	0.0	0.0	0.0	9.689082867403793E-5
7	2.768309390686798E-5	0.0	0.0	0.0	9.689082867403793E-5
8	4.152464086030197E-5	0.0	0.0	0.0	1.245739225809059E-4
9	4.152464086030197E-5	0.0	0.0	0.0	1.6609856344120788E-4
10	4.152464086030197E-5	0.0	0.0	0.0	1.9378165734807587E-4
11	5.536618781373596E-5	0.0	0.0	0.0	1.9378165734807587E-4
12	5.536618781373596E-5	0.0	0.0	5.536618781373596E-5	2.491478451618118E-4
13	5.536618781373596E-5	0.0	0.0	5.536618781373596E-5	2.768309390686798E-4
14	6.920773476716995E-5	0.0	0.0	6.920773476716995E-5	3.3219712688241577E-4
15	8.304928172060394E-5	1.384154695343399E-5	0.0	9.689082867403793E-5	4.290879555564537E-4
16	8.304928172060394E-5	1.384154695343399E-5	0.0	2.2146475125494385E-4	4.982956903236236E-4
17	8.304928172060394E-5	1.384154695343399E-5	0.0	5.675034250907936E-4	4.982956903236236E-4
18	8.304928172060394E-5	1.384154695343399E-5	0.0	8.028097232991715E-4	5.121372372770577E-4
19	8.304928172060394E-5	1.384154695343399E-5	0.0	0.0011626899440884552	5.121372372770577E-4
20	8.304928172060394E-5	1.384154695343399E-5	0.0	0.0017025102752723808	5.536618781373596E-4
21	9.689082867403793E-5	1.384154695343399E-5	0.0	0.00253300309247842	5.675034250907936E-4
22	9.689082867403793E-5	1.384154695343399E-5	0.0	0.004083256351263028	5.951865189976616E-4
23	9.689082867403793E-5	1.384154695343399E-5	0.0	0.0049967984501896705	7.197604415785675E-4
24	9.689082867403793E-5	1.384154695343399E-5	0.0	0.00679619955413609	7.197604415785675E-4
25	9.689082867403793E-5	1.384154695343399E-5	0.0	0.007778949387829903	7.197604415785675E-4
26	9.689082867403793E-5	1.384154695343399E-5	0.0	0.009232311817940472	7.751266293923035E-4
27	9.689082867403793E-5	1.384154695343399E-5	0.0	0.014782772146267503	7.751266293923035E-4
28	9.689082867403793E-5	1.384154695343399E-5	0.0	0.043517823621596466	7.751266293923035E-4
29	9.689082867403793E-5	1.384154695343399E-5	0.0	0.10173537010773984	7.751266293923035E-4
30	9.689082867403793E-5	1.384154695343399E-5	0.0	0.18672246840182455	7.751266293923035E-4
31	9.689082867403793E-5	1.384154695343399E-5	0.0	0.48992155441679613	7.889681763457375E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTATAC	4225	0.0	19.528994	37
AACGCAG	22570	0.0	15.057377	5
TCTTATA	7070	0.0	14.574965	37
TATACCG	315	0.0	13.507937	5
TAAGACT	1100	0.0	13.454546	4
GAGTACT	15985	0.0	13.36722	11
ACGCAGA	26025	0.0	13.093948	6
AGTACTT	16330	0.0	13.028169	12
CGCAGAG	26590	0.0	12.857466	7
CTCTTAT	11095	0.0	12.722398	37
GTACTTT	16820	0.0	12.681628	13
CGTTACG	540	0.0	12.675926	24
AGAGTAC	27105	0.0	12.544917	10
CAGAGTA	27665	0.0	12.384602	9
TACTTTT	17475	0.0	12.24864	14
GCAGAGT	28460	0.0	12.110155	8
ATACCGT	415	0.0	12.036144	6
CTCGTTA	910	0.0	11.994506	22
ACTTTTT	18255	0.0	11.796221	15
TACTTAC	1285	0.0	11.661479	31
>>END_MODULE