FastQCFastQC Report
Thu 9 Feb 2017
SRR2727337.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727337.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5255284
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT421460.8019737848611036No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT138960.264419582271862No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT137330.2613179420940904No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT101290.19273934577084703No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC30100.019.05315437
TATACTG6500.015.369235
TCTTATA50800.015.25885937
CGTATAC1353.9797123E-715.0740733
AACGCAG174850.014.8021155
CTATACT6150.014.7398394
GAGTACT119350.014.12107311
AGTACTT121950.013.82000712
TATACTA4450.013.7191025
CTCTTAT81750.013.48746137
GTATAAG6550.013.2748091
CTATACG1409.573541E-613.2142864
GTACTTT127250.013.142632513
TAGTACT3950.013.1139244
ACGCAGA200500.012.9730666
TAGATCG2305.7661964E-1012.8695655
CGCAGAG203950.012.798977
GTATTAC4650.012.7311821
TACTTTT131450.012.72270814
TATACCG2204.094545E-912.6136365
Produced by FastQC (version 0.11.5)