##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727337.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5255284
Sequences flagged as poor quality	0
Sequence length	43
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.1301813565166	33.0	31.0	34.0	30.0	34.0
2	32.27492500881018	34.0	31.0	34.0	30.0	34.0
3	32.35577563458036	34.0	31.0	34.0	30.0	34.0
4	35.92685304923578	37.0	35.0	37.0	35.0	37.0
5	35.80871024287175	37.0	35.0	37.0	35.0	37.0
6	35.82959417607117	37.0	35.0	37.0	35.0	37.0
7	35.769435486264875	37.0	35.0	37.0	35.0	37.0
8	35.746437490343055	37.0	35.0	37.0	35.0	37.0
9	37.46849627917349	39.0	37.0	39.0	35.0	39.0
10	37.313209333691574	39.0	37.0	39.0	34.0	39.0
11	37.418718379444385	39.0	37.0	39.0	35.0	39.0
12	37.32636162003804	39.0	37.0	39.0	34.0	39.0
13	37.38345596546257	39.0	37.0	39.0	34.0	39.0
14	38.544910988635436	40.0	38.0	41.0	34.0	41.0
15	38.50608435243462	40.0	38.0	41.0	34.0	41.0
16	38.441611718795784	40.0	38.0	41.0	34.0	41.0
17	38.409383774502004	40.0	38.0	41.0	34.0	41.0
18	38.37140885249969	40.0	38.0	41.0	34.0	41.0
19	38.38008259877107	40.0	38.0	41.0	34.0	41.0
20	38.268055732097444	40.0	38.0	41.0	34.0	41.0
21	38.19467758545495	40.0	38.0	41.0	34.0	41.0
22	38.121588671516136	40.0	38.0	41.0	33.0	41.0
23	38.009586922419416	40.0	37.0	41.0	33.0	41.0
24	37.902053438025426	40.0	37.0	41.0	33.0	41.0
25	37.81724964816364	40.0	37.0	41.0	33.0	41.0
26	37.461884077054634	39.0	37.0	41.0	32.0	41.0
27	37.24613569885091	39.0	37.0	41.0	31.0	41.0
28	37.09777397377573	39.0	36.0	41.0	31.0	41.0
29	37.045094232776	39.0	36.0	40.0	31.0	41.0
30	36.95766908125232	39.0	36.0	40.0	31.0	41.0
31	36.74383439600981	39.0	36.0	40.0	30.0	41.0
32	36.62936065871987	39.0	36.0	40.0	30.0	41.0
33	36.44458986422047	39.0	35.0	40.0	30.0	41.0
34	36.304590960260185	39.0	35.0	40.0	30.0	41.0
35	36.15119430272465	39.0	35.0	40.0	29.0	41.0
36	35.99614502279991	38.0	35.0	40.0	28.0	41.0
37	35.75349191404308	38.0	35.0	40.0	27.0	41.0
38	35.630922325035144	38.0	35.0	40.0	27.0	41.0
39	35.460284734374014	38.0	35.0	40.0	26.0	41.0
40	35.314672051976636	38.0	34.0	40.0	25.0	41.0
41	35.160847824779786	38.0	34.0	40.0	25.0	41.0
42	34.94803002083236	38.0	34.0	40.0	24.0	41.0
43	31.60656512569064	35.0	29.0	38.0	15.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	5.0
10	22.0
11	25.0
12	20.0
13	21.0
14	26.0
15	80.0
16	132.0
17	279.0
18	530.0
19	1146.0
20	2341.0
21	4589.0
22	7677.0
23	12822.0
24	19558.0
25	27823.0
26	38527.0
27	50923.0
28	66604.0
29	84911.0
30	106730.0
31	133554.0
32	166762.0
33	210469.0
34	271281.0
35	356506.0
36	502006.0
37	794979.0
38	1400590.0
39	994346.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.64886540860589	18.471085482725577	12.86297752890234	26.017071579766192
2	18.76444736383419	21.56313531295359	33.97955277012622	25.692864553085997
3	19.143932088161172	22.100099633055038	30.4364140929396	28.319554185844193
4	15.06082259303208	15.499276537671419	32.66660755156144	36.77329331773506
5	14.92459779528566	36.942989189547134	33.49221469286912	14.64019832229809
6	36.82164465326707	34.60492334952783	13.776572303228521	14.796859693976577
7	30.251666703455037	30.489389346037242	19.905013696690798	19.35393025381692
8	28.65749976594985	34.51767782673591	18.481646282103878	18.34317612521036
9	26.926670375949236	13.62466804838711	17.987705326676924	41.46095624898673
10	15.81202842700794	27.864488389209797	33.15145289959591	23.172030284186356
11	37.4924932696311	21.024839000137767	20.542067755044258	20.94059997518688
12	21.80510130375447	25.10615981933612	29.531172815779318	23.557566061130093
13	32.39255956481134	19.271917559545784	23.36081170874876	24.97471116689412
14	22.752338408352433	19.985180629629152	25.55648752760079	31.70599343441763
15	25.968568016495396	27.881385668215074	21.488600806350334	24.66144550893919
16	25.08794957608381	25.67815554782577	23.760028953715917	25.47386592237451
17	24.54908240924753	26.044187145737506	25.214603054754036	24.192127390260925
18	24.48706863415945	25.04009298070285	25.827471931107816	24.645366454029887
19	25.43050004528775	25.281868686830244	25.682760436924056	23.604870830957946
20	24.970962558826507	25.159553698715424	25.57212892776109	24.297354814696977
21	24.715695669349174	25.379028041110622	25.411909232688473	24.493367056851735
22	25.20731515175964	24.96805120332222	25.009209778196574	24.81542386672157
23	24.760431596085006	24.921412429851557	25.21932211465641	25.098833859407026
24	24.736018072477147	24.85686786860615	25.240462741880364	25.166651317036337
25	24.998915377361147	24.73835857396099	25.284114045977347	24.97861200270052
26	24.664356864443484	24.977698636267803	25.614257954470204	24.74368654481851
27	24.937700797901694	25.30700529219734	25.37583125859611	24.37946265130486
28	24.792513592034226	25.375526803118536	25.355356627729346	24.476602977117885
29	24.52135793232107	25.54027527342005	25.58160510450054	24.356761689758347
30	24.6591240359227	25.511161718377164	25.367477761430212	24.46223648426993
31	24.62072458881385	24.913173103489743	25.430176561342833	25.035925746353577
32	24.22841467749412	25.387019997396905	25.41584812542957	24.968717199679407
33	24.50897040007733	25.00041862628166	25.382015510484308	25.108595463156703
34	24.64782112631782	24.870149738815257	25.756286434757854	24.72574270010907
35	24.487239890365583	24.976366643553423	25.753070623775994	24.783322842305004
36	24.22481829716529	24.594484332340556	26.12859742689453	25.05209994359962
37	24.02117563960387	24.28131381672237	26.416802593351758	25.280707950322
38	23.73150528116083	24.32576431644798	26.837712291095972	25.105018111295223
39	23.566699725457273	24.11968601506598	27.283853736544017	25.029760522932726
40	23.74318876011268	24.030727930212713	27.53333597194747	24.692747337727134
41	23.379745033760308	23.928240605074816	27.705315259841335	24.986699101323545
42	23.00688221607053	24.06986948754815	27.76074518522691	25.16250311115441
43	23.16540837754915	23.398126533218758	27.641398638018423	25.79506645121367
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	827.0
1	881.5
2	936.0
3	2324.0
4	3712.0
5	3712.0
6	4102.5
7	4493.0
8	4515.5
9	4538.0
10	6771.0
11	9004.0
12	9004.0
13	18343.5
14	27683.0
15	45817.0
16	63951.0
17	46561.0
18	29171.0
19	29171.0
20	34281.0
21	39391.0
22	32958.5
23	26526.0
24	32915.0
25	39304.0
26	39304.0
27	48326.0
28	57348.0
29	66566.5
30	75785.0
31	87938.0
32	100091.0
33	100091.0
34	114850.5
35	129610.0
36	150384.5
37	171159.0
38	196550.5
39	221942.0
40	221942.0
41	249475.5
42	277009.0
43	304375.5
44	331742.0
45	359853.0
46	387964.0
47	387964.0
48	417015.0
49	446066.0
50	460558.5
51	475051.0
52	473205.5
53	471360.0
54	471360.0
55	447693.0
56	424026.0
57	397231.0
58	370436.0
59	342281.5
60	314127.0
61	314127.0
62	277645.5
63	241164.0
64	205193.5
65	169223.0
66	142921.5
67	116620.0
68	116620.0
69	96997.0
70	77374.0
71	65323.0
72	53272.0
73	43880.5
74	34489.0
75	34489.0
76	28480.0
77	22471.0
78	18836.0
79	15201.0
80	12182.0
81	9163.0
82	9163.0
83	7382.0
84	5601.0
85	4508.5
86	3416.0
87	2756.0
88	2096.0
89	2096.0
90	1594.5
91	1093.0
92	798.5
93	504.0
94	362.5
95	221.0
96	221.0
97	158.5
98	96.0
99	62.0
100	28.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	5255284.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.08610158017042
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.18363671955265	46.34457332420037
2	9.811274409157033	11.201748151041297
3	3.3513456197460245	5.7394576943724225
4	1.5518182155408355	3.543490091452918
5	0.9410067714628527	2.6859204071678318
6	0.6191592617537285	2.1207233106466004
7	0.42887396873691147	1.7137920061084315
8	0.3181422539482027	1.4529200820665182
9	0.23852614738489528	1.2254875091226751
>10	1.4342874899084739	15.070387581986164
>50	0.09117608746780635	3.5110346382459063
>100	0.028855810258751672	2.7803262978497134
>500	0.0011649770608986065	0.4208750558715858
>1k	5.991283808233482E-4	0.6710033466537257
>5k	0.0	0.0
>10k+	1.3313964018061056E-4	1.5182605032138474
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	42146	0.8019737848611036	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	13896	0.264419582271862	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	13733	0.2613179420940904	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	10129	0.19273934577084703	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	1.9028467348291737E-5
7	0.0	0.0	0.0	0.0	1.9028467348291737E-5
8	0.0	0.0	0.0	0.0	1.9028467348291737E-5
9	0.0	0.0	0.0	1.9028467348291737E-5	7.611386939316695E-5
10	1.9028467348291737E-5	0.0	0.0	3.8056934696583475E-5	9.51423367414587E-5
11	1.9028467348291737E-5	0.0	0.0	3.8056934696583475E-5	1.1417080408975043E-4
12	1.9028467348291737E-5	0.0	0.0	7.611386939316695E-5	1.7125620613462565E-4
13	1.9028467348291737E-5	0.0	0.0	7.611386939316695E-5	1.902846734829174E-4
14	3.8056934696583475E-5	0.0	0.0	1.1417080408975043E-4	3.2348394492095957E-4
15	5.7085402044875215E-5	0.0	0.0	2.0931314083120911E-4	3.425124122692513E-4
16	5.7085402044875215E-5	0.0	0.0	3.805693469658348E-4	3.995978143141265E-4
17	7.611386939316695E-5	0.0	0.0	7.611386939316696E-4	3.995978143141265E-4
18	7.611386939316695E-5	0.0	0.0	8.372525633248365E-4	4.3765474901071E-4
19	7.611386939316695E-5	0.0	0.0	0.0010275372368077538	4.3765474901071E-4
20	9.51423367414587E-5	1.9028467348291737E-5	0.0	0.0015222773878633392	4.947401510555852E-4
21	9.51423367414587E-5	1.9028467348291737E-5	0.0	0.0023595299511881755	5.137686184038769E-4
22	9.51423367414587E-5	1.9028467348291737E-5	0.0	0.0034631810573890966	5.327970857521687E-4
23	9.51423367414587E-5	1.9028467348291737E-5	0.0	0.004129177414579307	5.898824877970439E-4
24	1.1417080408975043E-4	1.9028467348291737E-5	0.0	0.0055753409330494795	5.898824877970439E-4
25	1.1417080408975043E-4	1.9028467348291737E-5	1.9028467348291737E-5	0.006469678898419191	6.089109551453356E-4
26	1.1417080408975043E-4	1.9028467348291737E-5	1.9028467348291737E-5	0.007934870884237655	6.279394224936273E-4
27	1.1417080408975043E-4	1.9028467348291737E-5	1.9028467348291737E-5	0.013300898676455924	6.279394224936273E-4
28	1.1417080408975043E-4	1.9028467348291737E-5	1.9028467348291737E-5	0.04003589530080582	6.279394224936273E-4
29	1.1417080408975043E-4	1.9028467348291737E-5	1.9028467348291737E-5	0.09940471342747605	6.279394224936273E-4
30	1.1417080408975043E-4	1.9028467348291737E-5	1.9028467348291737E-5	0.1846903040825196	6.279394224936273E-4
31	1.1417080408975043E-4	1.9028467348291737E-5	1.9028467348291737E-5	0.4567593302283949	6.279394224936273E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTATAC	3010	0.0	19.053154	37
TATACTG	650	0.0	15.36923	5
TCTTATA	5080	0.0	15.258859	37
CGTATAC	135	3.9797123E-7	15.074073	3
AACGCAG	17485	0.0	14.802115	5
CTATACT	615	0.0	14.739839	4
GAGTACT	11935	0.0	14.121073	11
AGTACTT	12195	0.0	13.820007	12
TATACTA	445	0.0	13.719102	5
CTCTTAT	8175	0.0	13.487461	37
GTATAAG	655	0.0	13.274809	1
CTATACG	140	9.573541E-6	13.214286	4
GTACTTT	12725	0.0	13.1426325	13
TAGTACT	395	0.0	13.113924	4
ACGCAGA	20050	0.0	12.973066	6
TAGATCG	230	5.7661964E-10	12.869565	5
CGCAGAG	20395	0.0	12.79897	7
GTATTAC	465	0.0	12.731182	1
TACTTTT	13145	0.0	12.722708	14
TATACCG	220	4.094545E-9	12.613636	5
>>END_MODULE