FastQCFastQC Report
Thu 9 Feb 2017
SRR2727336.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727336.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4862112
Sequences flagged as poor quality0
Sequence length43
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT137810.2834364983776597No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC22000.017.91136437
TCTTATA41000.015.88292637
TAAGACT7700.013.6948054
AACGCAG81750.013.4195725
CTCTTAT64050.012.99765737
GAGTACT40300.012.9454111
TAGGACT7250.012.7586214
GTATAGA6100.012.7377051
GTCTTAG7700.012.4935061
AGTACTT42800.012.27570112
ACGCAGA90700.012.1157676
ATACCGT3250.011.9538476
TAGTACG2654.2564352E-1011.8679242
GTACTTT43700.011.85354713
AGAGTAC92400.011.85281510
TACTTTT45050.011.78579414
TTAGACA7950.011.635224
CGCAGAG95400.011.59643657
CAGAGTA96800.011.5816129
CTTAGAC8000.011.5625013
Produced by FastQC (version 0.11.5)