##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727336.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4862112
Sequences flagged as poor quality	0
Sequence length	43
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.632507848441172	31.0	31.0	34.0	30.0	34.0
2	31.801702428903326	31.0	31.0	34.0	30.0	34.0
3	31.877469708636905	31.0	31.0	34.0	30.0	34.0
4	35.586878294864455	37.0	35.0	37.0	33.0	37.0
5	35.3158431973595	37.0	35.0	37.0	33.0	37.0
6	35.317536905772634	37.0	35.0	37.0	32.0	37.0
7	35.268685295608165	37.0	35.0	37.0	32.0	37.0
8	35.21053093799567	37.0	35.0	37.0	32.0	37.0
9	36.86971484819765	39.0	37.0	39.0	33.0	39.0
10	36.60656829789195	38.0	35.0	39.0	32.0	39.0
11	36.75173134637787	38.0	37.0	39.0	33.0	39.0
12	36.62689938035158	38.0	35.0	39.0	32.0	39.0
13	36.708337652444044	38.0	35.0	39.0	32.0	39.0
14	37.73462088080242	39.0	37.0	40.0	33.0	41.0
15	37.66939367089857	39.0	37.0	40.0	33.0	41.0
16	37.5796941740544	39.0	37.0	40.0	32.0	41.0
17	37.50602701048433	39.0	37.0	40.0	32.0	41.0
18	37.43445584963901	39.0	36.0	40.0	32.0	41.0
19	37.42500954317794	39.0	36.0	40.0	32.0	41.0
20	37.290481996301196	39.0	36.0	40.0	32.0	41.0
21	37.18755902784633	39.0	36.0	40.0	31.0	41.0
22	37.09810880539157	39.0	36.0	40.0	31.0	41.0
23	36.96389881598778	39.0	36.0	40.0	31.0	41.0
24	36.836116486004435	39.0	36.0	40.0	31.0	41.0
25	36.73522761302085	39.0	36.0	40.0	30.0	41.0
26	36.32921536978169	38.0	35.0	40.0	30.0	41.0
27	36.0643786074858	38.0	35.0	40.0	29.0	41.0
28	35.894993574808645	38.0	35.0	40.0	28.0	41.0
29	35.83660516253019	38.0	35.0	40.0	28.0	41.0
30	35.74961930124193	38.0	35.0	40.0	27.0	41.0
31	35.5142623205718	38.0	34.0	40.0	27.0	41.0
32	35.40249072830901	38.0	34.0	40.0	27.0	41.0
33	35.20562175449681	38.0	34.0	40.0	26.0	41.0
34	35.05133653852482	38.0	34.0	40.0	25.0	41.0
35	34.87703862025391	38.0	33.0	40.0	25.0	41.0
36	34.70684858760966	38.0	33.0	40.0	24.0	41.0
37	34.4237389019422	38.0	33.0	40.0	24.0	41.0
38	34.30509210812092	38.0	33.0	40.0	24.0	41.0
39	34.11810711065479	38.0	33.0	40.0	23.0	40.0
40	33.973310363891244	37.0	32.0	40.0	23.0	40.0
41	33.828841663869525	37.0	32.0	40.0	22.0	40.0
42	33.61381843939424	37.0	32.0	39.0	21.0	40.0
43	30.327153508598734	34.0	26.0	38.0	12.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	4.0
10	17.0
11	20.0
12	19.0
13	41.0
14	70.0
15	146.0
16	306.0
17	617.0
18	1107.0
19	2160.0
20	4447.0
21	7841.0
22	13186.0
23	20305.0
24	29267.0
25	40860.0
26	54354.0
27	70386.0
28	89454.0
29	111368.0
30	137657.0
31	169861.0
32	207502.0
33	257618.0
34	327179.0
35	430866.0
36	602997.0
37	898209.0
38	1099158.0
39	285089.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.95894705839767	18.854522479120185	12.562873911584102	26.623656550898044
2	19.557776538261564	21.065722056587756	33.48254832467866	25.893953080472027
3	19.270720213767188	21.866588017717405	30.015762697362792	28.846929071152616
4	15.704080860333946	15.696841208100512	32.64552523676954	35.953552694796
5	15.011089830921213	36.5170321045669	33.71006262299182	14.761815441520062
6	37.28283100019086	34.66989242534931	13.637448088402735	14.409828486057089
7	31.02993102585872	30.391566463298254	19.500250097077153	19.07825241376587
8	28.605593618575632	34.57197201545337	18.20910336907089	18.613330996900114
9	27.35825501345917	13.328405433688076	17.293061122409355	42.0202784304434
10	15.737913894208935	27.369587537267755	33.380164833718354	23.512333734804958
11	38.519351261344866	20.355351748376012	20.39329822102	20.731998769259118
12	22.303188408658624	24.41007117894446	29.12709538570893	24.15964502668799
13	32.44567381417787	19.08296230115637	22.826808596757953	25.644555287907806
14	23.140376034118507	19.419894070724823	24.44108650726269	32.998643387893985
15	26.404163458184428	27.367571952270946	20.66272846038923	25.565536129155397
16	25.968077247089333	25.54445475546429	22.86806638761098	25.619401609835396
17	25.37531015328318	25.63258518109003	24.05261334991872	24.93949131570807
18	25.34602246924793	24.925341086342726	24.554781954837736	25.17385448957161
19	26.276358915631725	24.901771082196376	24.424180273922115	24.39768972824978
20	25.998372723622985	24.666934862874403	24.192490835258422	25.14220157824419
21	25.763063458842577	24.855659433595935	24.134614751778653	25.246662355782835
22	26.282631909754446	24.41821578770707	23.779481015657396	25.519671286881092
23	25.76723859919311	24.41506900704879	23.929251321236535	25.888441072521573
24	25.667940187309547	24.42771783126345	23.900621787404322	26.003720194022677
25	25.917255711098385	24.13695941187698	24.067956476527073	25.87782840049756
26	25.616912979380153	24.362766633101007	24.2663270611619	25.753993326356937
27	25.960117743071326	24.61226726163445	23.950209291764565	25.47740570352966
28	25.684167703253237	24.78293383616009	24.03829858300261	25.49459987758406
29	25.625345528856595	24.884782580080426	24.107815698198642	25.382056192864333
30	25.846648534628574	24.79615854180241	23.85605267834225	25.501140245226768
31	25.72626874905391	24.27120148610316	24.07100042121613	25.931529343626803
32	25.40363117920772	24.630284123442653	24.04568220559296	25.92040249175667
33	25.535590294917103	24.25546758281175	24.158904607709573	26.050037514561573
34	25.688815889062198	24.168221546521348	24.510891563172546	25.632071001243904
35	25.559509941358815	24.160056370564888	24.518871634384396	25.761562053691893
36	25.314698633022026	23.90115653444429	24.81230378897072	25.97184104356296
37	25.160938291836963	23.680779052395337	25.05670375343061	26.101578902337092
38	24.891528619661578	23.616342034079018	25.44414855108233	26.04798079517707
39	24.69042259824537	23.451969020869942	25.829310390217252	26.028297990667433
40	24.851648830796165	23.262709703108445	26.181688122363283	25.703953343732106
41	24.457684232695588	23.19276067684167	26.39591601345259	25.953639077010155
42	24.129863730000462	23.220300149400096	26.475696158377264	26.174139962222178
43	24.411202374605935	22.440721233900003	26.344641999197055	26.80343439229701
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	239.0
1	251.5
2	264.0
3	574.0
4	884.0
5	884.0
6	1125.5
7	1367.0
8	1494.5
9	1622.0
10	2240.0
11	2858.0
12	2858.0
13	5886.5
14	8915.0
15	15455.5
16	21996.0
17	16814.5
18	11633.0
19	11633.0
20	14428.5
21	17224.0
22	16870.5
23	16517.0
24	21138.5
25	25760.0
26	25760.0
27	32602.0
28	39444.0
29	47408.5
30	55373.0
31	66880.0
32	78387.0
33	78387.0
34	92606.5
35	106826.0
36	126376.5
37	145927.0
38	170945.0
39	195963.0
40	195963.0
41	222546.5
42	249130.0
43	277950.5
44	306771.0
45	336020.5
46	365270.0
47	365270.0
48	394892.5
49	424515.0
50	440503.0
51	456491.0
52	456081.0
53	455671.0
54	455671.0
55	438727.0
56	421783.0
57	398378.5
58	374974.0
59	346828.0
60	318682.0
61	318682.0
62	281639.0
63	244596.0
64	208639.0
65	172682.0
66	145111.0
67	117540.0
68	117540.0
69	97709.5
70	77879.0
71	65705.5
72	53532.0
73	43797.5
74	34063.0
75	34063.0
76	27996.5
77	21930.0
78	18209.5
79	14489.0
80	11625.5
81	8762.0
82	8762.0
83	7055.5
84	5349.0
85	4282.0
86	3215.0
87	2569.5
88	1924.0
89	1924.0
90	1444.5
91	965.0
92	708.0
93	451.0
94	312.0
95	173.0
96	173.0
97	117.0
98	61.0
99	38.0
100	15.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	4862112.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	61.1832919929179
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.57672557352734	50.523159125841744
2	9.329826543705714	11.416590033336453
3	2.967552456050317	5.4469388526848155
4	1.4593102041611394	3.5714160931774255
5	0.8438393834434886	2.5814435696173392
6	0.5538776648051309	2.03328353364767
7	0.37522279424263055	1.6070156047781794
8	0.30396115270075064	1.4877875168155146
9	0.21847965781510167	1.2030574228752737
>10	1.2779265500527726	14.316584995918324
>50	0.07117844055419838	2.924616580560472
>100	0.021427260834033648	2.1129461615444414
>500	4.7062308283138464E-4	0.19296262863262914
>1k	1.6807918705421369E-4	0.2987602505795597
>5k	0.0	0.0
>10k+	3.3615837410840855E-5	0.2834376299901424
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	13781	0.2834364983776597	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.0567193844979302E-5
2	0.0	0.0	0.0	0.0	2.0567193844979302E-5
3	0.0	0.0	0.0	0.0	2.0567193844979302E-5
4	0.0	0.0	0.0	0.0	2.0567193844979302E-5
5	0.0	0.0	0.0	0.0	6.17015815349379E-5
6	0.0	0.0	0.0	0.0	6.17015815349379E-5
7	0.0	0.0	0.0	0.0	6.17015815349379E-5
8	0.0	0.0	0.0	0.0	6.17015815349379E-5
9	0.0	0.0	0.0	2.0567193844979302E-5	1.0283596922489651E-4
10	0.0	2.0567193844979302E-5	0.0	2.0567193844979302E-5	1.0283596922489651E-4
11	2.0567193844979302E-5	2.0567193844979302E-5	0.0	2.0567193844979302E-5	1.0283596922489651E-4
12	2.0567193844979302E-5	2.0567193844979302E-5	0.0	2.0567193844979302E-5	2.2623913229477232E-4
13	2.0567193844979302E-5	2.0567193844979302E-5	0.0	2.0567193844979302E-5	2.673735199847309E-4
14	2.0567193844979302E-5	2.0567193844979302E-5	0.0	4.1134387689958603E-5	2.879407138297102E-4
15	2.0567193844979302E-5	2.0567193844979302E-5	0.0	4.1134387689958603E-5	3.2907510151966883E-4
16	2.0567193844979302E-5	2.0567193844979302E-5	0.0	8.226877537991721E-5	3.907766830546067E-4
17	2.0567193844979302E-5	2.0567193844979302E-5	0.0	8.226877537991721E-5	4.319110707445653E-4
18	2.0567193844979302E-5	2.0567193844979302E-5	0.0	1.0283596922489651E-4	5.141798461244826E-4
19	4.1134387689958603E-5	2.0567193844979302E-5	0.0	1.439703569148551E-4	5.347470399694618E-4
20	4.1134387689958603E-5	2.0567193844979302E-5	0.0	4.1134387689958603E-4	6.581502030393377E-4
21	4.1134387689958603E-5	2.0567193844979302E-5	0.0	5.758814276594204E-4	6.581502030393377E-4
22	4.1134387689958603E-5	2.0567193844979302E-5	0.0	9.872253045590065E-4	6.992845907292962E-4
23	4.1134387689958603E-5	2.0567193844979302E-5	0.0	0.0012134644368537787	9.049565291790893E-4
24	4.1134387689958603E-5	4.1134387689958603E-5	0.0	0.0018099130583581785	9.255237230240686E-4
25	4.1134387689958603E-5	6.17015815349379E-5	0.0	0.002097853772187889	9.666581107140272E-4
26	4.1134387689958603E-5	6.17015815349379E-5	0.0	0.0026531680060023297	9.872253045590065E-4
27	4.1134387689958603E-5	6.17015815349379E-5	0.0	0.0060878893781138735	9.872253045590065E-4
28	4.1134387689958603E-5	6.17015815349379E-5	0.0	0.02651111286617832	9.872253045590065E-4
29	4.1134387689958603E-5	6.17015815349379E-5	0.0	0.06873556182992083	0.0010077924984039858
30	4.1134387689958603E-5	6.17015815349379E-5	0.0	0.13025203862025392	0.0010077924984039858
31	4.1134387689958603E-5	6.17015815349379E-5	0.0	0.3518224179122159	0.0010077924984039858
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTATAC	2200	0.0	17.911364	37
TCTTATA	4100	0.0	15.882926	37
TAAGACT	770	0.0	13.694805	4
AACGCAG	8175	0.0	13.419572	5
CTCTTAT	6405	0.0	12.997657	37
GAGTACT	4030	0.0	12.94541	11
TAGGACT	725	0.0	12.758621	4
GTATAGA	610	0.0	12.737705	1
GTCTTAG	770	0.0	12.493506	1
AGTACTT	4280	0.0	12.275701	12
ACGCAGA	9070	0.0	12.115767	6
ATACCGT	325	0.0	11.953847	6
TAGTACG	265	4.2564352E-10	11.867924	2
GTACTTT	4370	0.0	11.853547	13
AGAGTAC	9240	0.0	11.852815	10
TACTTTT	4505	0.0	11.785794	14
TTAGACA	795	0.0	11.63522	4
CGCAGAG	9540	0.0	11.5964365	7
CAGAGTA	9680	0.0	11.581612	9
CTTAGAC	800	0.0	11.562501	3
>>END_MODULE